;hsqct1etf3gpsi3d ;avance-version (07/04/04) ;3D H-1/X correlation via double inept transfer ; using sensitivity improvement ;for measuring N-15 T1 relaxation times ;phase sensitive using Echo/Antiecho-TPPI gradient selection ;with decoupling during acquisition ;using f3 - channel ;using flip-back pulse ;as pseudo3D ;(use parameterset HSQCT1ETF3GPSI3D) ; ;$CLASS=HighRes ;$DIM=3D ;$TYPE= ;$SUBTYPE= ;$COMMENT= prosol relations= #include #include #include "p2=p1*2" "p22=p21*2" "d11=30m" "d24=1s/(cnst4*cnst11)" "d25=1s/(cnst4*cnst12)" "d26=1s/(cnst4*4)" "d10=3u" "in10=inf2/2" "DELTA2=p16+d16+8u" "DELTA3=d25-p16-d16-4u" # ifdef LABEL_CN "DELTA1=d25-p16-d16-larger(p2,p8)-d10*2" # else "DELTA1=d25-p16-d16-p2-d10*2" # endif /*LABEL_CN*/ "spoff1=0" "spoff13=bf2*((cnst21+cnst22)/2000000)-o2" aqseq 312 1 ze d11 pl16:f3 st0 2 6m do:f3 3 3m 4 d1 "TAU=vd-p26*2-p16-d16-8u" (p1 ph1) d26 pl3:f3 (center (p2 ph1) (p22 ph1):f3 ) d26 UNBLKGRAD (p1 ph2) 4u pl0:f1 (p11:sp1 ph1:r):f1 4u p16:gp1 d16 pl1:f1 (p21 ph3):f3 d25 (center (p2 ph1) (p22 ph1):f3 ) d25 pl19:f1 (p21 ph2):f3 (p26 ph2):f1 TAU cpds1:f1 ph1 4u do:f1 (p26 ph7):f1 4u p16:gp4 d16 pl1:f1 (p21 ph8):f3 4u p16:gp2*-1*EA d16 DELTA3 (p22 ph6):f3 d10 # ifdef LABEL_CN (center (p2 ph9) (p8:sp13 ph1):f2 ) # else (p2 ph9) # endif /*LABEL_CN*/ d10 p16:gp2*EA d16 DELTA1 (center (p1 ph1) (p21 ph4):f3 ) d24 (center (p2 ph1) (p22 ph1):f3 ) d24 (center (p1 ph2) (p21 ph5):f3 ) d26 (center (p2 ph1) (p22 ph1):f3 ) d26 (p1 ph1) DELTA2 (p2 ph1) 4u p16:gp3 d16 pl16:f3 4u BLKGRAD goscnp ph31 cpd3:f3 3m do:f3 3m st ivd lo to 3 times nbl 3m ipp3 ipp4 ipp5 ipp8 ipp9 ipp31 lo to 4 times ns d1 mc #0 to 4 F1QF() F2EA(igrad EA & ip5*2 & rpp3 rpp4 rpp5 rpp8 rpp9 rpp31, id10 & ip6*2 & ip8*2 & ip31*2) exit ph0=0 ph1=0 ph2=1 ph3=0 0 0 0 2 2 2 2 ph4=0 0 2 2 ph5=3 3 1 1 ph6=0 ph7=3 ph8=1 3 ph9=0 0 2 2 ph31=0 2 2 0 2 0 0 2 ;pl0 : 120dB ;pl1 : f1 channel - power level for pulse (default) ;pl3 : f3 channel - power level for pulse (default) ;pl16: f3 channel - power level for CPD/BB decoupling ;pl19: f1 channel - power level for CPD/BB decoupling ;sp1 : f1 channel - shaped pulse 90 degree ;sp13: f2 channel - shaped pulse 180 degree (Ca and C=O, adiabatic) ;p1 : f1 channel - 90 degree high power pulse ;p2 : f1 channel - 180 degree high power pulse ;p8 : f2 channel - 180 degree shaped pulse for inversion (adiabatic) ;p11: f1 channel - 90 degree shaped pulse ;p16: homospoil/gradient pulse ;p21: f3 channel - 90 degree high power pulse ;p22: f3 channel - 180 degree high power pulse ;p26: f1 channel - 90 degree pulse at pl19 ;d1 : relaxation delay; 1-5 * T1 ;d10: incremented delay [3 usec] ;d11: delay for disk I/O [30 msec] ;d16: delay for homospoil/gradient recovery ;d24: 1/(4J)YH for YH ; 1/(8J)YH for all multiplicities ;d25: 1/(4J)YH for YH ; 1/(8J)YH for all multiplicities ;d26: 1/(4J(YH)) ;vd : variable delay, taken from vd-list ;cnst4: = J(YH) ;cnst11: for multiplicity selection = 4 for NH, 8 for all multiplicities ;cnst12: for multiplicity selection = 4 for NH, 8 for all multiplicities ;cnst21: CO chemical shift (offset, in ppm) ;cnst22: Calpha chemical shift (offset, in ppm) ;inf2: 1/SW(X) = 2 * DW(X) ;in10: 1/(2 * SW(X)) = DW(X) ;nd10: 2 ;NS: 8 * n ;DS: >= 16 ;td1: number of delays in vd-list ;td2: number of experiments in F2 ;NBL: = td1 ;FnMODE: QF in F1 ;FnMODE: echo-antiecho in F2 ;cpds1: decoupling according to sequence defined by cpdprg1 ;cpd3: decoupling according to sequence defined by cpdprg3 ;pcpd1: f1 channel - 90 degree pulse for decoupling sequence ;pcpd3: f3 channel - 90 degree pulse for decoupling sequence ;use gradient ratio: gp 1 : gp 2 : gp 3 : gp 4 ; 50 : 80 : 16.2 : 30 for N-15 ;for z-only gradients: ;gpz1: 50% ;gpz2: 80% ;gpz3: 16.2% ;gpz4: 30% ;use gradient files: ;gpnam1: SINE.100 ;gpnam2: SINE.100 ;gpnam3: SINE.100 ;gpnam4: SINE.100 ;preprocessor-flags-start ;LABEL_CN: for C-13 and N-15 labeled samples start experiment with ; option -DLABEL_CN (eda: ZGOPTNS) ;preprocessor-flags-end ;$Id: hsqct1etf3gpsi3d,v 1.6.2.1 2007/12/14 16:06:46 ber Exp $