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...onto) equilibrated with the corresponding NMR buffer at a flow rate of 2.5 ml/min. The column is calibrated with LMW gel filtration calibration kit (17-0 ...echnology) assuming a specific refractive index increment (dn/dc) of 0.185 ml/g. To determine the detector delay volumes and the normalization coefficien

...mg/mL.  Prepare a sample of 10 mg/mL.  Prepare a sample of 10 mg/mL of phage as follows: ...mple is not aligned strongly enough, add phage to a concentration of 15 mg/mL and repeat.

...mb and pipette out any air bubbles. Then, fill outer part of case with 600 mL of RB.<br>

...-><font face="Verdana, Helvetica, Arial"><span style="font-size: 12px;">30 ml Lysis buffer</span></font> <!--EndFragment--> to the a frozen cell pellet a ...0.5g of NaCl, and 0.6g of ¹⁵N-labelled NH₄Cl, in 500 mL water and autoclave the media.&nbsp; <br>

:'''ASLA Biotech P-50-P''', Pf1 phage 50 mg/mL ...ock 0.5-1mM and a pf1 phage stock of 50 mg/mL. Prepare a sample of 12.5 mg/mL of phage.

**Requires large sample volumes - 0.4 to 0.6 ml. **Ideal for small sample volumes: 0.2 to 0.3 ml

...ont face="Verdana, Helvetica, Arial"><span style="font-size: 12px;">(10 mg/ml SA in 50% ACN/50% 0.1% TFA).</span></font>