View source for Spin System Identification with CARA

== '''Spin System Identification with CARA''' ==

Spin system identification in 2D [15N,1H]-HSQC and 3D HNCO

# Open a repositiry or create one if it does not exist (see the CARA page).
# Open 2D [15N,1H]-HSQC in SynchroScope to determine the region with true peak region and noise level.
# Run Lua script '''Pick_2D_Peaks'''. This script should be in the repository, otherwise get it from http://www.cara.ethz.ch/Wiki/AllCaluaScripts . '''Pick_2D_Peaks''' is equivalent to the <tt>in</tt> command in XEASY, but is not interactive. The script will create spin systems and populate them with H and N spins. Note that in CARA spin systems are distinct from residues and can have numbers starting with 1.
# To delete unwanted spin systems in SynchroScope use '''right click -> Delete Systems'''. To pick additional spin systems use '''right click -> Pick System'''
# Open 3D HNCO in the strip panels of SynchroScope by using '''Strips -> Select Spectrum''' or '''right click -> Select Spectrum''' 
# Select a spin system by left-clicking in HSQC plane or using '''View -> Goto System''' - this updates the strip display.
# In the strip panel set the cursor on a peak and use '''right click -> Pick Label...''' or '''right click -> Pick Spin''' and '''right click -> Label Spin''' to pick C-1. Repeat last two steps for all spin systems.

Also see instructions on how to use SynchroScope here:
http://www.cara.ethz.ch/Wiki/SynchroScope

%COMMENT%




-- Main.AlexEletski - 06 Jul 2007