CYANA - Revision history

TheresaRamelot at 22:55, 1 February 2013

2013-02-01T22:55:30Z

← Older revision		Revision as of 22:55, 1 February 2013
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	*[[Media:CYANA3ex_PfR193A_n15.peaks\|filename1.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_ali.peaks\|filename2.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_aro.peaks\|filename3.peaks]]:  NOESY peak lists in XEASY format.  The peak lists are unassigned.		*[[Media:CYANA3ex_PfR193A_n15.peaks\|filename1.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_ali.peaks\|filename2.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_aro.peaks\|filename3.peaks]]:  NOESY peak lists in XEASY format.  The peak lists are unassigned.
	*[[Media:CYANA3ex_ssa.cya\|ssa.cya]]:  file with stereospecific assignments defined. Leu and Val methyl group stereospecific assignments come from the U-15N, 5% biosynthetically directed 13C sample data.A description of how to obtain the LEU and VAL stereospecific assignments is found here: [[SSAFromFractional13CLabeledSample\|'''SSA from <sup>13</sup>C fractionally labeled sample''']].   Stereospecific assignments for ASN HD21 / HD22 and GLN HD21 / HE22 come from NOEs to either the ASN HBs or GLN HGs.  The NH2 proton with the strongest NOEs to the HGs or HBs is assigned to the HD21 or HE22, respectively.		*[[Media:CYANA3ex_ssa.cya\|ssa.cya]]:  file with stereospecific assignments defined. Leu and Val methyl group stereospecific assignments come from the U-15N, 5% biosynthetically directed 13C sample data.A description of how to obtain the LEU and VAL stereospecific assignments is found here: [[SSAFromFractional13CLabeledSample\|'''SSA from <sup>13</sup>C fractionally labeled sample''']].   <br>Stereospecific assignments for ASN HD21 / HD22 and GLN HD21 / HE22 come from NOEs to either the ASN HBs or GLN HGs.  The NH2 proton with the strongest NOEs to the HGs or HBs is assigned to the HD21 or HE22, respectively.

	*other:  other constraint files such as, manual upper and lower distance limits, [[HydrogenBonds\|hydrogen bond constraints]].		*other:  other constraint files such as, manual upper and lower distance limits, [[HydrogenBonds\|hydrogen bond constraints]].

TheresaRamelot at 22:38, 1 February 2013

2013-02-01T22:38:41Z

← Older revision		Revision as of 22:38, 1 February 2013
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	*[[Media:CYANA3ex_PfR193A_h2o.prot\|filename.prot]]:  chemical shift assignment list.  You should make all degenerate geminal proton assignments Q's, as well as degenerate side chain aromatics (HD1/HD2 and HE1/HE2).		*[[Media:CYANA3ex_PfR193A_h2o.prot\|filename.prot]]:  chemical shift assignment list.  You should make all degenerate geminal proton assignments Q's, as well as degenerate side chain aromatics (HD1/HD2 and HE1/HE2).

	~~   if~~ your assignments are in a bmrb file (2.1), start cyana, read in the bmrb file, and then write the shifts out to a prot file as follows:		If your assignments are in a bmrb file (2.1), start cyana, read in the bmrb file, and then write the shifts out to a prot file as follows:
	<pre> Open project in cyana 3.0:		<pre> Open project in cyana 3.0:
	read bmrb [finename.bmrb]		read bmrb [finename.bmrb]

TheresaRamelot at 22:35, 1 February 2013

2013-02-01T22:35:14Z

← Older revision		Revision as of 22:35, 1 February 2013
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	Distance modification does not affect Phe and Tyr ring atoms HD1/2 and HE1/2. Therefore, if you have degenerate ring chemical shift (as is almost always the case) make sure you have them labeled QD and QE		Distance modification does not affect Phe and Tyr ring atoms HD1/2 and HE1/2. Therefore, if you have degenerate ring chemical shift (as is almost always the case) make sure you have them labeled QD and QE

	External stereospecific assignments determined with [[GLOMSA\|'''GLOMSA''']] or with the help of a fractionally (i.e., 5%) <sup>13</sup>C-labeled sample [3] can be defined ~~with a custom macro~~ like this:<br>		External stereospecific assignments determined with [[GLOMSA\|'''GLOMSA''']] or with the help of a fractionally (i.e., 5%) <sup>13</sup>C-labeled sample [3] can be defined in the file ssa.cya like this:<br>
	<pre> # VAL		<pre> # VAL
	atom stereo "QG1 25 36 38 87"		atom stereo "QG1 25 36 38 87"

TheresaRamelot at 19:50, 1 February 2013

2013-02-01T19:50:47Z

← Older revision		Revision as of 19:50, 1 February 2013
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	read bmrb [finename.bmrb]		read bmrb [finename.bmrb]
	write prot [filename.prot]</pre>		write prot [filename.prot]</pre>
	You will then need to edit the filename.prot file to fix all degenerate geminal proton assignments: <br>         if degenerate HB2=HB3 then change HB2 to QB and delete HB3 line<br>         if degenerate HG2=HG3 then change HG2 to QG and delete HG3 line<br>                 etc. for QD and QE and fix degenerate aromatic protons (typically all F and Y HD1/2 and HE1/2: <br>		You will then need to edit the filename.prot file to fix all degenerate geminal proton assignments: <br>         if degenerate HB2=HB3 then change HB2 to QB and delete HB3 line<br>         if degenerate HG2=HG3 then change HG2 to QG and delete HG3 line<br>                 etc. for QD and QE and fix degenerate aromatic protons (typically all F and Y HD1/2 and HE1/2: <br>         if degenerate PHE/TYR HD1=HD2 then change HD1 to QD and delete HD2 line<br>         if degenerate PHE/TYR HE1=HE2 then change HE1 to QE and delete HE2 line <br> Degenerate aromatic side chain carbons don't need to be corrected. <br> Also fix degenerate VAL and LEU isopropyl methyl groups:<br>         if degenerate VAL QG1=QG2 then change QG1 to QQG and delete QG2 line<br>         if degenerate LEU QD1=QD2 then change QD1 to QQD and delete QD2 line<br>
	if degenerate PHE/TYR HD1=HD2 then change HD1 to QD and delete HD2 line<br>         if degenerate PHE/TYR HE1=HE2 then change HE1 to QE and delete HE2 line <br>
	Degenerate aromatic side chain carbons don't need to be corrected. <br>
	Also fix degenerate VAL and LEU isopropyl methyl groups:<br>
	if degenerate VAL QG1=QG2 then change QG1 to QQG and delete QG2 line<br>         if degenerate LEU QD1=QD2 then change QD1 to QQD and delete QD2 line<br>

	*[[Media:CYANA3ex_PfR193A_n15.peaks\|filename1.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_ali.peaks\|filename2.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_aro.peaks\|filename3.peaks]]:  NOESY peak lists in XEASY format.  The peak lists are unassigned.		*[[Media:CYANA3ex_PfR193A_n15.peaks\|filename1.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_ali.peaks\|filename2.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_aro.peaks\|filename3.peaks]]:  NOESY peak lists in XEASY format.  The peak lists are unassigned.

TheresaRamelot at 19:49, 1 February 2013

2013-02-01T19:49:31Z

← Older revision		Revision as of 19:49, 1 February 2013
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	*[[Media:CYANA3ex_PfR193A_h2o.prot\|filename.prot]]:  chemical shift assignment list.  You should make all degenerate geminal proton assignments Q's, as well as degenerate side chain aromatics (HD1/HD2 and HE1/HE2).		*[[Media:CYANA3ex_PfR193A_h2o.prot\|filename.prot]]:  chemical shift assignment list.  You should make all degenerate geminal proton assignments Q's, as well as degenerate side chain aromatics (HD1/HD2 and HE1/HE2).

	if your assignments are in a bmrb file (2.1), start cyana, read in the bmrb file, and then write the shifts out to a prot file as follows:		if your assignments are in a bmrb file (2.1), start cyana, read in the bmrb file, and then write the shifts out to a prot file as follows:
	<pre> Open project in cyana 3.0:		<pre> Open project in cyana 3.0:
	read bmrb [finename.bmrb]		read bmrb [finename.bmrb]
	write prot [filename.prot]</pre>		write prot [filename.prot]</pre>
	You will then need to edit the filename.prot file to fix all degenerate geminal proton assignments: ~~ ~~		You will then need to edit the filename.prot file to fix all degenerate geminal proton assignments: <br>         if degenerate HB2=HB3 then change HB2 to QB and delete HB3 line<br>         if degenerate HG2=HG3 then change HG2 to QG and delete HG3 line<br>                 etc. for QD and QE and fix degenerate aromatic protons (typically all F and Y HD1/2 and HE1/2: <br>
	if degenerate HB2=HB3 then change HB2 to QB and delete HB3 line<br>		if degenerate PHE/TYR HD1=HD2 then change HD1 to QD and delete HD2 line<br>         if degenerate PHE/TYR HE1=HE2 then change HE1 to QE and delete HE2 line <br>
	if degenerate HG2=HG3 then change HG2 to QG and delete HG3 line<br>		Degenerate aromatic side chain carbons don't need to be corrected. <br>
	etc. for QD and QE		Also fix degenerate VAL and LEU isopropyl methyl groups:<br>
	and fix degenerate aromatic protons (typically all F and Y HD1/2 and HE1/2:		if degenerate VAL QG1=QG2 then change QG1 to QQG and delete QG2 line<br>         if degenerate LEU QD1=QD2 then change QD1 to QQD and delete QD2 line<br>
	if degenerate PHE/TYR HD1=HD2 then change HD1 to QD and delete HD2 line
	if degenerate PHE/TYR HE1=HE2 then change HE1 to QE and delete HE2 line
	Degenerate aromatic side chain carbons don't need to be corrected.
	Also fix degenerate VAL and LEU isopropyl methyl groups:
	if degenerate VAL QG1=QG2 then change QG1 to QQG and delete QG2 line<br>
	if degenerate LEU QD1=QD2 then change QD1 to QQD and delete QD2 line

	*[[Media:CYANA3ex_PfR193A_n15.peaks\|filename1.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_ali.peaks\|filename2.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_aro.peaks\|filename3.peaks]]:  NOESY peak lists in XEASY format.  The peak lists are unassigned.		*[[Media:CYANA3ex_PfR193A_n15.peaks\|filename1.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_ali.peaks\|filename2.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_aro.peaks\|filename3.peaks]]:  NOESY peak lists in XEASY format.  The peak lists are unassigned.

TheresaRamelot at 19:48, 1 February 2013

2013-02-01T19:48:14Z

TheresaRamelot at 19:46, 1 February 2013

2013-02-01T19:46:01Z

@@ Line 305: / Line 305: @@
 		read bmrb [finename.bmrb]
 		write prot [filename.prot]</pre>
-*[[Media:CYANA3ex_PfR193A_n15.peaks|filename1.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_ali.peaks|filename2.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_aro.peaks|filename3.peaks]]:&nbsp; NOESY peak lists in XEASY format.&nbsp; The peak lists are unassigned.
+You will then need to edit the filename.prot file to fix all degenerate geminal proton assignments:
 *[[Media:CYANA3ex_ssa.cya|ssa.cya]]:&nbsp; file with stereospecific assignments defined. Leu and Val methyl group stereospecific assignments come from the U-15N, 5% biosynthetically directed 13C sample data.A description of how to obtain the LEU and VAL stereospecific assignments is found here: [[SSAFromFractional13CLabeledSample|'''SSA from <sup>13</sup>C fractionally labeled sample''']]. &nbsp; Stereospecific assignments for ASN HD21 / HD22 and GLN HD21 / HE22 come from NOEs to either the ASN HBs or GLN HGs.&nbsp; The NH2 proton with the strongest NOEs to the HGs or HBs is assigned to the HD21 or HE22, respectively.

TheresaRamelot at 17:12, 1 February 2013

2013-02-01T17:12:16Z

← Older revision		Revision as of 17:12, 1 February 2013
Line 308:		Line 308:
	*[[Media:CYANA3ex_ssa.cya\|ssa.cya]]:  file with stereospecific assignments defined. Leu and Val methyl group stereospecific assignments come from the U-15N, 5% biosynthetically directed 13C sample data.A description of how to obtain the LEU and VAL stereospecific assignments is found here: [[SSAFromFractional13CLabeledSample\|'''SSA from <sup>13</sup>C fractionally labeled sample''']].   Stereospecific assignments for ASN HD21 / HD22 and GLN HD21 / HE22 come from NOEs to either the ASN HBs or GLN HGs.  The NH2 proton with the strongest NOEs to the HGs or HBs is assigned to the HD21 or HE22, respectively.		*[[Media:CYANA3ex_ssa.cya\|ssa.cya]]:  file with stereospecific assignments defined. Leu and Val methyl group stereospecific assignments come from the U-15N, 5% biosynthetically directed 13C sample data.A description of how to obtain the LEU and VAL stereospecific assignments is found here: [[SSAFromFractional13CLabeledSample\|'''SSA from <sup>13</sup>C fractionally labeled sample''']].   Stereospecific assignments for ASN HD21 / HD22 and GLN HD21 / HE22 come from NOEs to either the ASN HBs or GLN HGs.  The NH2 proton with the strongest NOEs to the HGs or HBs is assigned to the HD21 or HE22, respectively.

	*other:  other constraint files such as, manual upper and lower distance limits, [HydrogenBonds \| hydrogen bond constraints].		*other:  other constraint files such as, manual upper and lower distance limits, [[HydrogenBonds\|hydrogen bond constraints]].

	==== Running the Program ====		==== Running the Program ====

TheresaRamelot at 17:09, 1 February 2013

2013-02-01T17:09:52Z

← Older revision		Revision as of 17:09, 1 February 2013
Line 308:		Line 308:
	*[[Media:CYANA3ex_ssa.cya\|ssa.cya]]:  file with stereospecific assignments defined. Leu and Val methyl group stereospecific assignments come from the U-15N, 5% biosynthetically directed 13C sample data.A description of how to obtain the LEU and VAL stereospecific assignments is found here: [[SSAFromFractional13CLabeledSample\|'''SSA from <sup>13</sup>C fractionally labeled sample''']].   Stereospecific assignments for ASN HD21 / HD22 and GLN HD21 / HE22 come from NOEs to either the ASN HBs or GLN HGs.  The NH2 proton with the strongest NOEs to the HGs or HBs is assigned to the HD21 or HE22, respectively.		*[[Media:CYANA3ex_ssa.cya\|ssa.cya]]:  file with stereospecific assignments defined. Leu and Val methyl group stereospecific assignments come from the U-15N, 5% biosynthetically directed 13C sample data.A description of how to obtain the LEU and VAL stereospecific assignments is found here: [[SSAFromFractional13CLabeledSample\|'''SSA from <sup>13</sup>C fractionally labeled sample''']].   Stereospecific assignments for ASN HD21 / HD22 and GLN HD21 / HE22 come from NOEs to either the ASN HBs or GLN HGs.  The NH2 proton with the strongest NOEs to the HGs or HBs is assigned to the HD21 or HE22, respectively.

	*other:  other constraint files such as, manual upper and lower distance limits, hydrogen bond constraints.		*other:  other constraint files such as, manual upper and lower distance limits, [HydrogenBonds \| hydrogen bond constraints].

	==== Running the Program ====		==== Running the Program ====

TheresaRamelot at 17:07, 1 February 2013

2013-02-01T17:07:12Z

← Older revision		Revision as of 17:07, 1 February 2013
Line 306:		Line 306:
	write prot [filename.prot]</pre>		write prot [filename.prot]</pre>
	*[[Media:CYANA3ex_PfR193A_n15.peaks\|filename1.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_ali.peaks\|filename2.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_aro.peaks\|filename3.peaks]]:  NOESY peak lists in XEASY format.  The peak lists are unassigned.		*[[Media:CYANA3ex_PfR193A_n15.peaks\|filename1.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_ali.peaks\|filename2.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_aro.peaks\|filename3.peaks]]:  NOESY peak lists in XEASY format.  The peak lists are unassigned.
	*[[Media:CYANA3ex_ssa.cya\|ssa.cya]]:  file with stereospecific assignments defined. Leu and Val methyl group stereospecific assignments come from the U-15N, 5% biosynthetically directed 13C sample data.  Stereospecific assignments for ASN HD21 / HD22 and GLN HD21 / HE22 come from NOEs to either the ASN HBs or GLN HGs.  The NH2 proton with the strongest NOEs to the HGs or HBs is assigned to the HD21 or HE22, respectively.		*[[Media:CYANA3ex_ssa.cya\|ssa.cya]]:  file with stereospecific assignments defined. Leu and Val methyl group stereospecific assignments come from the U-15N, 5% biosynthetically directed 13C sample data.A description of how to obtain the LEU and VAL stereospecific assignments is found here: [[SSAFromFractional13CLabeledSample\|'''SSA from <sup>13</sup>C fractionally labeled sample''']].   Stereospecific assignments for ASN HD21 / HD22 and GLN HD21 / HE22 come from NOEs to either the ASN HBs or GLN HGs.  The NH2 proton with the strongest NOEs to the HGs or HBs is assigned to the HD21 or HE22, respectively.

	*other:  other constraint files such as, manual upper and lower distance limits, hydrogen bond constraints.		*other:  other constraint files such as, manual upper and lower distance limits, hydrogen bond constraints.

← Older revision		Revision as of 22:55, 1 February 2013
Line 309:		Line 309:

	*[[Media:CYANA3ex_PfR193A_n15.peaks\|filename1.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_ali.peaks\|filename2.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_aro.peaks\|filename3.peaks]]:  NOESY peak lists in XEASY format.  The peak lists are unassigned.		*[[Media:CYANA3ex_PfR193A_n15.peaks\|filename1.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_ali.peaks\|filename2.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_aro.peaks\|filename3.peaks]]:  NOESY peak lists in XEASY format.  The peak lists are unassigned.
	*[[Media:CYANA3ex_ssa.cya\|ssa.cya]]:  file with stereospecific assignments defined. Leu and Val methyl group stereospecific assignments come from the U-15N, 5% biosynthetically directed 13C sample data.A description of how to obtain the LEU and VAL stereospecific assignments is found here: [[SSAFromFractional13CLabeledSample\|'''SSA from <sup>13</sup>C fractionally labeled sample''']].   Stereospecific assignments for ASN HD21 / HD22 and GLN HD21 / HE22 come from NOEs to either the ASN HBs or GLN HGs.  The NH2 proton with the strongest NOEs to the HGs or HBs is assigned to the HD21 or HE22, respectively.		*[[Media:CYANA3ex_ssa.cya\|ssa.cya]]:  file with stereospecific assignments defined. Leu and Val methyl group stereospecific assignments come from the U-15N, 5% biosynthetically directed 13C sample data.A description of how to obtain the LEU and VAL stereospecific assignments is found here: [[SSAFromFractional13CLabeledSample\|'''SSA from <sup>13</sup>C fractionally labeled sample''']].   <br>Stereospecific assignments for ASN HD21 / HD22 and GLN HD21 / HE22 come from NOEs to either the ASN HBs or GLN HGs.  The NH2 proton with the strongest NOEs to the HGs or HBs is assigned to the HD21 or HE22, respectively.

	*other:  other constraint files such as, manual upper and lower distance limits, [[HydrogenBonds\|hydrogen bond constraints]].		*other:  other constraint files such as, manual upper and lower distance limits, [[HydrogenBonds\|hydrogen bond constraints]].

← Older revision		Revision as of 19:48, 1 February 2013
Line 300:		Line 300:
	*[[Media:CYANA3ex_PfR193A.seq\|PROT.seq]]:  protein sequence file.		*[[Media:CYANA3ex_PfR193A.seq\|PROT.seq]]:  protein sequence file.
	*[[Media:CYANA3ex_PfR193A.aco\|PROT.aco]]:  dihedral angle constraint file.		*[[Media:CYANA3ex_PfR193A.aco\|PROT.aco]]:  dihedral angle constraint file.
	*[[Media:CYANA3ex_PfR193A_h2o.prot\|filename.prot]]:  chemical shift assignment list.  You should make all degenerate geminal proton assignments Q's, as well as degenerate side chain aromatics (HD1/HD2 and HE1/HE2).		*[[Media:CYANA3ex_PfR193A_h2o.prot\|filename.prot]]:  chemical shift assignment list.  You should make all degenerate geminal proton assignments Q's, as well as degenerate side chain aromatics (HD1/HD2 and HE1/HE2).
	**if your assignments are in a bmrb file (2.1), start cyana, read in the bmrb file, and then write the shifts out to a prot file as follows:
			if your assignments are in a bmrb file (2.1), start cyana, read in the bmrb file, and then write the shifts out to a prot file as follows:
	<pre> Open project in cyana 3.0:		<pre> Open project in cyana 3.0:
	read bmrb [finename.bmrb]		read bmrb [finename.bmrb]
	write prot [filename.prot]</pre>		write prot [filename.prot]</pre>
	You will then need to edit the filename.prot file to fix all degenerate geminal proton assignments:		You will then need to edit the filename.prot file to fix all degenerate geminal proton assignments:
			if degenerate HB2=HB3 then change HB2 to QB and delete HB3 line<br>
	if degenerate HB2=HB3 then change HB2 to QB and delete HB3 line		if degenerate HG2=HG3 then change HG2 to QG and delete HG3 line<br>

	if degenerate HG2=HG3 then change HG2 to QG and delete HG3 line

	etc. for QD and QE		etc. for QD and QE

	and fix degenerate aromatic protons (typically all F and Y HD1/2 and HE1/2:		and fix degenerate aromatic protons (typically all F and Y HD1/2 and HE1/2:

	if degenerate PHE/TYR HD1=HD2 then change HD1 to QD and delete HD2 line		if degenerate PHE/TYR HD1=HD2 then change HD1 to QD and delete HD2 line

	if degenerate PHE/TYR HE1=HE2 then change HE1 to QE and delete HE2 line		if degenerate PHE/TYR HE1=HE2 then change HE1 to QE and delete HE2 line

	Degenerate aromatic side chain carbons don't need to be corrected.		Degenerate aromatic side chain carbons don't need to be corrected.

	Also fix degenerate VAL and LEU isopropyl methyl groups:		Also fix degenerate VAL and LEU isopropyl methyl groups:

	if degenerate VAL QG1=QG2 then change QG1 to QQG and delete QG2 line<br>		if degenerate VAL QG1=QG2 then change QG1 to QQG and delete QG2 line<br>

	if degenerate LEU QD1=QD2 then change QD1 to QQD and delete QD2 line		if degenerate LEU QD1=QD2 then change QD1 to QQD and delete QD2 line

	*[[Media:CYANA3ex_PfR193A_n15.peaks\|filename1.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_ali.peaks\|filename2.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_aro.peaks\|filename3.peaks]]:  NOESY peak lists in XEASY format.  The peak lists are unassigned.		*[[Media:CYANA3ex_PfR193A_n15.peaks\|filename1.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_ali.peaks\|filename2.peaks]], [[Media:CYANA3ex_PfR193A_c13_h2o_aro.peaks\|filename3.peaks]]:  NOESY peak lists in XEASY format.  The peak lists are unassigned.
	*[[Media:CYANA3ex_ssa.cya\|ssa.cya]]:  file with stereospecific assignments defined. Leu and Val methyl group stereospecific assignments come from the U-15N, 5% biosynthetically directed 13C sample data.A description of how to obtain the LEU and VAL stereospecific assignments is found here: [[SSAFromFractional13CLabeledSample\|'''SSA from <sup>13</sup>C fractionally labeled sample''']].   Stereospecific assignments for ASN HD21 / HD22 and GLN HD21 / HE22 come from NOEs to either the ASN HBs or GLN HGs.  The NH2 proton with the strongest NOEs to the HGs or HBs is assigned to the HD21 or HE22, respectively.		*[[Media:CYANA3ex_ssa.cya\|ssa.cya]]:  file with stereospecific assignments defined. Leu and Val methyl group stereospecific assignments come from the U-15N, 5% biosynthetically directed 13C sample data.A description of how to obtain the LEU and VAL stereospecific assignments is found here: [[SSAFromFractional13CLabeledSample\|'''SSA from <sup>13</sup>C fractionally labeled sample''']].   Stereospecific assignments for ASN HD21 / HD22 and GLN HD21 / HE22 come from NOEs to either the ASN HBs or GLN HGs.  The NH2 proton with the strongest NOEs to the HGs or HBs is assigned to the HD21 or HE22, respectively.