Common NMR experiment sets - Revision history

Alex at 11:07, 8 January 2010

2010-01-08T11:07:13Z

← Older revision		Revision as of 11:07, 8 January 2010
Line 3:		Line 3:
	The following experiments are recorded for NC samples		The following experiments are recorded for NC samples

	Common experiments		{\| cellspacing="0" cellpadding="0"
			\|-
			! Common experiments
			! GFT protocol
			! Conventional protocol
			! Fractionally 13C-labeled samples
			\|- valign="top"
			\|
	*1D		*1D
	*1D 15N T1		*1D 15N T1
Line 20:		Line 26:
	*3D 15N/13Cali/13Caro-resolved [1H,1H]-NOESY		*3D 15N/13Cali/13Caro-resolved [1H,1H]-NOESY

	~~<br>~~		\|

	~~GFT protocol~~

	*(3,2)D GFT HN<u>NCO</u> or 3D HNCO<br>		*(3,2)D GFT HN<u>NCO</u> or 3D HNCO<br>
	*(4,3)D GFT <u>CABCA</u>(CO)NH<br>		*(4,3)D GFT <u>CABCA</u>(CO)NH<br>
Line 31:		Line 34:
	*(4,3)D <u>GFT HC</u>CH-COSY aromatic		*(4,3)D <u>GFT HC</u>CH-COSY aromatic

	~~<br>~~		\|

	~~Conventional protocol~~

	*3D HNCO		*3D HNCO
	*3D HN(CA)CO		*3D HN(CA)CO
Line 43:		Line 43:
	*(H)CCH-COSY aromatic		*(H)CCH-COSY aromatic

	~~<br>~~		\|

	~~Fractionally 13C-labeled samples~~

	*2D [13C,1H]-CT-HSQC, 28ms delay - optimized for methyl groups<br>		*2D [13C,1H]-CT-HSQC, 28ms delay - optimized for methyl groups<br>
	*2D [13C,1H]-CT-HSQC, 56ms delay (optional)<br>		*2D [13C,1H]-CT-HSQC, 56ms delay (optional)<br>
	*2D [13C,1H]-CT-HSQC, 42ms delay (optional)		*2D [13C,1H]-CT-HSQC, 42ms delay (optional)
	*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional - can also be run for the NC sample)<br>		*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional - can also be run for the NC sample)<br>
			\|}
	~~<br>~~

	== Arrowsmith group ==		== Arrowsmith group ==

Agutmana at 22:02, 23 December 2009

2009-12-23T22:02:41Z

← Older revision		Revision as of 22:02, 23 December 2009
Line 60:		Line 60:
	<br>		<br>

	{\| cellspacing="0"		{\| cellspacing="0" cellpadding="0"
	\|-		\|-
	! NH-rooted		! NH-rooted

Agutmana at 22:01, 23 December 2009

2009-12-23T22:01:13Z

← Older revision		Revision as of 22:01, 23 December 2009
Line 60:		Line 60:
	<br>		<br>

	{\|		{\| cellspacing="0"
	\|-		\|-
	! NH-rooted		! NH-rooted

Agutmana at 21:52, 23 December 2009

2009-12-23T21:52:53Z

← Older revision		Revision as of 21:52, 23 December 2009
Line 58:		Line 58:
	Data acquisition is taylored for [[Abacus\|ABACUS]] approach for resonance assignment. Most 3D spectra are collected with [[Setting up non-uniformly sampled spectra\|Non-uniform sampling]] and processed with [[Processing non-uniformly sampled spectra with Multidimensional Decomposition\|Multidimensional decomposition]],		Data acquisition is taylored for [[Abacus\|ABACUS]] approach for resonance assignment. Most 3D spectra are collected with [[Setting up non-uniformly sampled spectra\|Non-uniform sampling]] and processed with [[Processing non-uniformly sampled spectra with Multidimensional Decomposition\|Multidimensional decomposition]],

			<br>

	{\|		{\|
	\|-		\|-
	! NH-rooted		! NH-rooted
	! CH-rooted~~ ~~		! CH-rooted (aliphatic)
	! ~~Aromatic~~		! CH-rooted (aromatic)
	! Other		! Other
	\|- valign="top"		\|- valign="top"
Line 77:		Line 78:

	\|		\|
	*2D [13C,1H]-HSQC ~~aliphatic~~		*2D [13C,1H]-HSQC
	*2D [13C,1H]-CT-HSQC ~~aliphatic~~		*2D [13C,1H]-CT-HSQC
	*3D 13C-NOESY-HSQC		*3D 13C-NOESY-HSQC
	*3D (H)CCH-TOCSY		*3D (H)CCH-TOCSY
	*3D H(C)CH-TOCSY		*3D H(C)CH-TOCSY

	\|		\|
	*2D [13C,1H]-HSQC ~~aromatic~~		*2D [13C,1H]-HSQC
	*2D [13C,1H]-CT-HSQC ~~aromatic~~ (optional)		*2D [13C,1H]-CT-HSQC (optional)
	*3D 13C-NOESY-HSQC		*3D 13C-NOESY-HSQC
	*3D (H)CCH-TOCSY (optional)		*3D (H)CCH-TOCSY (optional)
	*3D H(C)CH-TOCSY (optional)		*3D H(C)CH-TOCSY (optional)
	\|
			\|
	*1D presaturation		*1D presaturation
	*1D 15N T1		*1D 15N T1
	*1D 15N T2		*1D 15N T2
	*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)		*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
	*2D HE(CECDCG)CB (optional)		*2D HE(CECDCG)CB (optional)
			*2D HD(CDCG)CB (optional)

	\|}		\|}

Agutmana at 21:49, 23 December 2009

2009-12-23T21:49:44Z

← Older revision		Revision as of 21:49, 23 December 2009
Line 58:		Line 58:
	Data acquisition is taylored for [[Abacus\|ABACUS]] approach for resonance assignment. Most 3D spectra are collected with [[Setting up non-uniformly sampled spectra\|Non-uniform sampling]] and processed with [[Processing non-uniformly sampled spectra with Multidimensional Decomposition\|Multidimensional decomposition]],		Data acquisition is taylored for [[Abacus\|ABACUS]] approach for resonance assignment. Most 3D spectra are collected with [[Setting up non-uniformly sampled spectra\|Non-uniform sampling]] and processed with [[Processing non-uniformly sampled spectra with Multidimensional Decomposition\|Multidimensional decomposition]],

	*1D presaturation
	*1D 15N T1
	*1D 15N T2
	*2D [15N,1H]-HSQC
	*2D [13C,1H]-HSQC aliphatic
	*2D [13C,1H]-HSQC aromatic
	*2D [13C,1H]-CT-HSQC aliphatic
	*2D [13C,1H]-CT-HSQC aromatic (optional)
	*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
	*2D HE(CECDCG)CB (optional)

	{\|		{\|
	\|-		\|-
	! NH-rooted		! NH-rooted
	! CH-rooted		! CH-rooted
	! Aromatic		! Aromatic
			! Other
	\|- valign="top"		\|- valign="top"
	\|		\|
			*2D [15N,1H]-HSQC
	*3D 15N-NOESY-HSQC		*3D 15N-NOESY-HSQC
	*3D HNCO		*3D HNCO
Line 85:		Line 77:

	\|		\|
			*2D [13C,1H]-HSQC aliphatic
			*2D [13C,1H]-CT-HSQC aliphatic
			*3D 13C-NOESY-HSQC
	*3D (H)CCH-TOCSY		*3D (H)CCH-TOCSY
	*3D H(C)CH-TOCSY		*3D H(C)CH-TOCSY
	*3D 13C-NOESY-HSQC

	\|		\|
			*2D [13C,1H]-HSQC aromatic
			*2D [13C,1H]-CT-HSQC aromatic (optional)
			*3D 13C-NOESY-HSQC
	*3D (H)CCH-TOCSY (optional)		*3D (H)CCH-TOCSY (optional)
	*3D H(C)CH-TOCSY (optional)		*3D H(C)CH-TOCSY (optional)
	*~~3D 13C~~-~~NOESY~~-HSQC		\|
			*1D presaturation
			*1D 15N T1
			*1D 15N T2
			*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
			*2D HE(CECDCG)CB (optional)

	\|}		\|}

Agutmana at 21:47, 23 December 2009

2009-12-23T21:47:28Z

← Older revision		Revision as of 21:47, 23 December 2009
Line 69:		Line 69:
	*2D HE(CECDCG)CB (optional)		*2D HE(CECDCG)CB (optional)

	{\|		{\|
	\|- ~~valign="top"~~		\|-
	! NH-rooted		! NH-rooted
	! CH-rooted		! CH-rooted
	! Aromatic		! Aromatic
	\|-		\|- valign="top"
	\|		\|
	*3D 15N-NOESY-HSQC		*3D 15N-NOESY-HSQC

Agutmana at 21:46, 23 December 2009

2009-12-23T21:46:58Z

← Older revision		Revision as of 21:46, 23 December 2009
Line 69:		Line 69:
	*2D HE(CECDCG)CB (optional)		*2D HE(CECDCG)CB (optional)

	{\|~~align~~="top"		{\|
	\|-		\|- valign="top"
	! NH-rooted		! NH-rooted
	! CH-rooted		! CH-rooted
	! Aromatic		! Aromatic
	\|-		\|-

Agutmana at 21:43, 23 December 2009

2009-12-23T21:43:52Z

← Older revision		Revision as of 21:43, 23 December 2009
Line 56:		Line 56:
	== Arrowsmith group ==		== Arrowsmith group ==

	Data acquisition is taylored for [[Abacus\|ABACUS]] approach for resonance assignment. Most 3D spectra collected with [[Setting up non-uniformly sampled spectra\|Non-uniform sampling]] and processed with [[Processing non-uniformly sampled spectra with Multidimensional Decomposition\|Multidimensional decomposition]],		Data acquisition is taylored for [[Abacus\|ABACUS]] approach for resonance assignment. Most 3D spectra are collected with [[Setting up non-uniformly sampled spectra\|Non-uniform sampling]] and processed with [[Processing non-uniformly sampled spectra with Multidimensional Decomposition\|Multidimensional decomposition]],

	*1D presaturation		*1D presaturation
Line 69:		Line 69:
	*2D HE(CECDCG)CB (optional)		*2D HE(CECDCG)CB (optional)

	{\|		{\|align="top"
	!NH-rooted !!CH-rooted !!Aromatic		\|-
	\|-		! NH-rooted
			! CH-rooted
			! Aromatic
			\|-
	\|		\|
	*3D 15N-NOESY-HSQC		*3D 15N-NOESY-HSQC
Line 77:		Line 80:
	*3D HNCA		*3D HNCA
	*3D CBCA(CO)NH		*3D CBCA(CO)NH
	*3D HBHA(CO)NH		*3D HBHA(CO)NH
	*3D C(CCO)NH-TOCSY (optional)		*3D C(CCO)NH-TOCSY (optional)
	*3D H(CCCO)NH-TOCSY (optional)		*3D H(CCCO)NH-TOCSY (optional)

	\|		\|
	*3D (H)CCH-TOCSY		*3D (H)CCH-TOCSY
	*3D H(C)CH-TOCSY		*3D H(C)CH-TOCSY
	*3D 13C-NOESY-HSQC		*3D 13C-NOESY-HSQC

	\|		\|
	*3D (H)CCH-TOCSY (optional)		*3D (H)CCH-TOCSY (optional)
	*3D H(C)CH-TOCSY (optional)		*3D H(C)CH-TOCSY (optional)
	*3D 13C-NOESY-HSQC		*3D 13C-NOESY-HSQC

	\|}		\|}

Agutmana at 21:42, 23 December 2009

2009-12-23T21:42:06Z

← Older revision		Revision as of 21:42, 23 December 2009
Line 69:		Line 69:
	*2D HE(CECDCG)CB (optional)		*2D HE(CECDCG)CB (optional)

	{!NH-rooted !!CH-rooted !!Aromatic		{\|
			!NH-rooted !!CH-rooted !!Aromatic
	\|-		\|-
	\|		\|
Line 77:		Line 78:
	*3D CBCA(CO)NH		*3D CBCA(CO)NH
	*3D HBHA(CO)NH		*3D HBHA(CO)NH
	\|\|		*3D C(CCO)NH-TOCSY (optional)
			*3D H(CCCO)NH-TOCSY (optional)
			\|
	*3D (H)CCH-TOCSY		*3D (H)CCH-TOCSY
	*3D H(C)CH-TOCSY		*3D H(C)CH-TOCSY
	*3D 13C-NOESY-HSQC		*3D 13C-NOESY-HSQC
	\|\|		\|
	*3D (H)CCH-TOCSY (optional)		*3D (H)CCH-TOCSY (optional)
	*3D H(C)CH-TOCSY (optional)		*3D H(C)CH-TOCSY (optional)
	*3D 13C-NOESY-HSQC		*3D 13C-NOESY-HSQC
	\|}		\|}

Agutmana at 21:39, 23 December 2009

2009-12-23T21:39:53Z

← Older revision		Revision as of 21:39, 23 December 2009
Line 60:		Line 60:
	*1D presaturation		*1D presaturation
	*1D 15N T1		*1D 15N T1
	*1D 15N T2		*1D 15N T2
	*2D [15N,1H]-HSQC		*2D [15N,1H]-HSQC
	*2D [13C,1H]-HSQC aliphatic		*2D [13C,1H]-HSQC aliphatic
Line 66:		Line 66:
	*2D [13C,1H]-CT-HSQC aliphatic		*2D [13C,1H]-CT-HSQC aliphatic
	*2D [13C,1H]-CT-HSQC aromatic (optional)		*2D [13C,1H]-CT-HSQC aromatic (optional)
	*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)		*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
	*2D HE(CECDCG)CB (optional)		*2D HE(CECDCG)CB (optional)

	{!NH-rooted		{!NH-rooted !!CH-rooted !!Aromatic
	!!CH-rooted		\|-
	!!Aromatic		\|
	\|-		*3D 15N-NOESY-HSQC
	\|
	*3D 15N-NOESY-HSQC
	*3D HNCO		*3D HNCO
	*3D HNCA		*3D HNCA
	*3D CBCA(CO)NH		*3D CBCA(CO)NH
	*3D HBHA(CO)NH		*3D HBHA(CO)NH
	\|\|		\|\|
	*3D (H)CCH-TOCSY		*3D (H)CCH-TOCSY
	*3D H(C)CH-TOCSY		*3D H(C)CH-TOCSY
	*3D 13C-NOESY-HSQC		*3D 13C-NOESY-HSQC
	\|\|		\|\|
	*3D (H)CCH-TOCSY (optional)		*3D (H)CCH-TOCSY (optional)
	*3D H(C)CH-TOCSY (optional)		*3D H(C)CH-TOCSY (optional)
	*3D 13C-NOESY-HSQC		*3D 13C-NOESY-HSQC
			\|}