Conditioning procedure for cryogenic probes - Revision history

Alex: /* Cryogenic Probe Conditioning */

2014-02-12T18:18:34Z

Cryogenic Probe Conditioning

← Older revision		Revision as of 18:18, 12 February 2014
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	##Enter <tt>pwxcal</tt>, at the prompts select decoupler channel and nucleus.		##Enter <tt>pwxcal</tt>, at the prompts select decoupler channel and nucleus.
	##For channel 2 (13C) enter <tt>pw=0 pwx1=200 pwx2=0 temp=25 at=0.1 d1=0.1 nt=3000 dp='y' dm='nnn' dm2='nnn'</tt> <br>For channel 3 (15N) enter <tt>pw=0 pwx1=0 pwx2=200 temp=25 at=0.1 d1=0.1 nt=3000 dp='y' dm='nnn' dm2='nnn'</tt>		##For channel 2 (13C) enter <tt>pw=0 pwx1=200 pwx2=0 temp=25 at=0.1 d1=0.1 nt=3000 dp='y' dm='nnn' dm2='nnn'</tt> <br>For channel 3 (15N) enter <tt>pw=0 pwx1=0 pwx2=200 temp=25 at=0.1 d1=0.1 nt=3000 dp='y' dm='nnn' dm2='nnn'</tt>
	##Set <tt>dpwr</tt> or <tt>dpwr2</tt> to the corresponding high-power level.		##Set <tt>pwxlvl1</tt> or <tt>pwxlvl2</tt> (<tt>dpwr</tt> or <tt>dpwr2</tt>, in older VNMRJ versions) to the corresponding high-power level.
	##Run experiment with <tt>go</tt>		##Run experiment with <tt>go</tt>
	#When completed, replace the sample in the magnet. Either monitor the real data as it comes in or set up the experiment but run the first increment a number of times using <tt>array('nt',200,1,0)</tt> to see if the conditioning has been successful.		#When completed, replace the sample in the magnet. Either monitor the real data as it comes in or set up the experiment but run the first increment a number of times using <tt>array('nt',200,1,0)</tt> to see if the conditioning has been successful.

Alex: /* Cryogenic Probe Conditioning */

2012-05-09T17:55:47Z

Cryogenic Probe Conditioning

← Older revision		Revision as of 17:55, 9 May 2012
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	#X Nucleus Channels - Do this procedure for both X Nucleus Channels.		#X Nucleus Channels - Do this procedure for both X Nucleus Channels.
	##Enter <tt>pwxcal</tt>, at the prompts select decoupler channel and nucleus.		##Enter <tt>pwxcal</tt>, at the prompts select decoupler channel and nucleus.
	##For channel 2 (13C) enter <tt>pw=0 pwx1=200 pwx2=0 temp=25 at=0.1 d1=0.1 nt=3000 dp='y'</tt> <br>For channel 3 (15N) enter <tt>pw=0 pwx1=0 pwx2=200 temp=25 at=0.1 d1=0.1 nt=3000 dp='y'</tt>		##For channel 2 (13C) enter <tt>pw=0 pwx1=200 pwx2=0 temp=25 at=0.1 d1=0.1 nt=3000 dp='y' dm='nnn' dm2='nnn'</tt> <br>For channel 3 (15N) enter <tt>pw=0 pwx1=0 pwx2=200 temp=25 at=0.1 d1=0.1 nt=3000 dp='y' dm='nnn' dm2='nnn'</tt>
	##Set <tt>dpwr</tt> or <tt>dpwr2</tt> to the corresponding high-power level.		##Set <tt>dpwr</tt> or <tt>dpwr2</tt> to the corresponding high-power level.
	##Run experiment with <tt>go</tt>		##Run experiment with <tt>go</tt>

Aringh at 15:56, 15 December 2009

2009-12-15T15:56:38Z

Alex: moved NESG:CryoProbeConditioning to Conditioning procedure for cryogenic probes

2009-11-30T19:35:36Z

moved NESG:CryoProbeConditioning to Conditioning procedure for cryogenic probes

← Older revision	Revision as of 19:35, 30 November 2009
(No difference)

Admin: Created page with 'The information below is taken from '''Decoupling Noise''' in [http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199919600c.pdf HCN Cold Probe manual] …'

2009-10-08T15:02:47Z

Created page with 'The information below is taken from '''Decoupling Noise''' in [http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199919600c.pdf HCN Cold Probe manual] …'

New page

The information below is taken from '''Decoupling Noise''' in [http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199919600c.pdf HCN Cold Probe manual]

== '''Cryogenic Probe Conditioning''' ==

NMR experiments that require X-nucleus decoupling perform best when the rf coils are conditioned. Conditioning imparts just enough energy into the rf coils to disperse any extraneous condensed material into the vacuum space so that the material is carried away by the pump.

Use the following procedures to condition the rf coils if the Cold Probe has been left idle for a number of days or thermal cycled. Both procedures (run in the order presented) are required during the initial installation of the probe.

# Install the probe in the magnet.
# Start cryogenic operations, refer to the Cryogenic Systems Operation and Installation manual.
# Determine the approximate 90º pulse width and powers for all channels. Refer to the contract for pulse widths and use the procedures in Testing Probe NMR Performance, page 19.
# Take the sample out of the probe.
# Proton Channel
## Enter <tt>s2pul temp=25 tn='H1' pw=200 d1=0.1 at=0.1 nt=3000 dp='y'</tt>
## Set <tt>tpwr</tt> to the normal high-power level
## Run experiment with <tt>go</tt>
# X Nucleus Channels - Do this procedure for both X Nucleus Channels.
## Enter <tt>pwxcal</tt>, at the prompts select decoupler channel and nucleus.
## For channel 2 (13C) enter <tt>pw=0 pwx1=200 pwx2=0 temp=25 at=0.1 d1=0.1 nt=3000 dp='y'</tt> <br/>For channel 3 (15N) enter <tt>pw=0 pwx1=0 pwx2=200 temp=25 at=0.1 d1=0.1 nt=3000 dp='y'</tt>
## Set <tt>dpwr</tt> or <tt>dpwr2</tt> to the corresponding high-power level.
## Run experiment with <tt>go</tt>
# When completed, replace the sample in the magnet. Either monitor the real data as it comes in or set up the experiment but run the first increment a number of times using <tt>array('nt',200,1,0)</tt> to see if the conditioning has been successful.

== '''cryo_noisetest Macro Procedure (Lower Power, Longer Time)''' ==

This is an rf decoupling coil conditioning procedure that also quantifies the noise profile of a Cold Probe. The macro runs forever by cycling between periods of prolonged pulsing and testing.

# Remove the sample from the magnet.
# Ensure that the probe parameter is set to a valid probefile name.
# Enter <tt>cryo_noisetest</tt>. Enter the number of minutes that the decoupler coil conditioning will run before another quantitative test is done. Add five minutes (for the quantitative testing) to the number you entered to get a total recycle time. For example, 40 will run the conditioning for forty minutes followed by five minutes of tests and then start over. Results are printed out at the end of the tests. Reduce the number for more testing (per unit time), increase it for more.
# Enter desired number (use 40 during the initial installation of the probe). The procedure starts and a quantitative test is done straight away as a baseline measurement. Four mini-tests are done consecutively; two carbon and two nitrogen, if more than 2 channels are present. For each nucleus CW and WALTZ decoupling modes are selected. An array of <tt>dpwr</tt> and <tt>dpwr2</tt> is done for each nucleus and for each decoupling mode. The noise is estimated from each spectrum and saved in two date-stamped text files in the local users <tt>vnmrsys/data/testlib</tt> directory and also plotted out in graphical form.
# Enter <tt>aa</tt> or <tt>halt</tt> to stop the acquisition(s) and procedure.

-- Main.AlexEletski - 07 Mar 2008

← Older revision		Revision as of 15:56, 15 December 2009
Line 1:		Line 1:
	The information below is taken from '''Decoupling Noise''' in [http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199919600c.pdf HCN Cold Probe manual]		The information below is taken from '''Decoupling Noise''' in [http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199919600c.pdf HCN Cold Probe manual]

	== '''Cryogenic Probe Conditioning''' ==		== '''Cryogenic Probe Conditioning''' ==

	NMR experiments that require X-nucleus decoupling perform best when the rf coils are conditioned. Conditioning imparts just enough energy into the rf coils to disperse any extraneous condensed material into the vacuum space so that the material is carried away by the pump.		NMR experiments that require X-nucleus decoupling perform best when the rf coils are conditioned. Conditioning imparts just enough energy into the rf coils to disperse any extraneous condensed material into the vacuum space so that the material is carried away by the pump.

	Use the following procedures to condition the rf coils if the Cold Probe has been left idle for a number of days or thermal cycled. Both procedures (run in the order presented) are required during the initial installation of the probe.		Use the following procedures to condition the rf coils if the Cold Probe has been left idle for a number of days or thermal cycled. Both procedures (run in the order presented) are required during the initial installation of the probe.

	# Install the probe in the magnet.		#Install the probe in the magnet.
	# Start cryogenic operations, refer to the Cryogenic Systems Operation and Installation manual.		#Start cryogenic operations, refer to the Cryogenic Systems Operation and Installation manual.
	# Determine the approximate 90º pulse width and powers for all channels. Refer to the contract for pulse widths and use the procedures in Testing Probe NMR Performance, page 19.		#Determine the approximate 90º pulse width and powers for all channels. Refer to the contract for pulse widths and use the procedures in Testing Probe NMR Performance, page 19.
	# Take the sample out of the probe.		#Take the sample out of the probe.
	# Proton Channel		#Proton Channel
	## Enter <tt>s2pul temp=25 tn='H1' pw=200 d1=0.1 at=0.1 nt=3000 dp='y'</tt>		##Enter <tt>s2pul temp=25 tn='H1' pw=200 d1=0.1 at=0.1 nt=3000 dp='y'</tt>
	## Set <tt>tpwr</tt> to the normal high-power level		##Set <tt>tpwr</tt> to the normal high-power level
	## Run experiment with <tt>go</tt>		##Run experiment with <tt>go</tt>
	# X Nucleus Channels - Do this procedure for both X Nucleus Channels.		#X Nucleus Channels - Do this procedure for both X Nucleus Channels.
	## Enter <tt>pwxcal</tt>, at the prompts select decoupler channel and nucleus.		##Enter <tt>pwxcal</tt>, at the prompts select decoupler channel and nucleus.
	## For channel 2 (13C) enter <tt>pw=0 pwx1=200 pwx2=0 temp=25 at=0.1 d1=0.1 nt=3000 dp='y'</tt> <br/>For channel 3 (15N) enter <tt>pw=0 pwx1=0 pwx2=200 temp=25 at=0.1 d1=0.1 nt=3000 dp='y'</tt>		##For channel 2 (13C) enter <tt>pw=0 pwx1=200 pwx2=0 temp=25 at=0.1 d1=0.1 nt=3000 dp='y'</tt> <br>For channel 3 (15N) enter <tt>pw=0 pwx1=0 pwx2=200 temp=25 at=0.1 d1=0.1 nt=3000 dp='y'</tt>
	## Set <tt>dpwr</tt> or <tt>dpwr2</tt> to the corresponding high-power level.		##Set <tt>dpwr</tt> or <tt>dpwr2</tt> to the corresponding high-power level.
	## Run experiment with <tt>go</tt>		##Run experiment with <tt>go</tt>
	# When completed, replace the sample in the magnet. Either monitor the real data as it comes in or set up the experiment but run the first increment a number of times using <tt>array('nt',200,1,0)</tt> to see if the conditioning has been successful.		#When completed, replace the sample in the magnet. Either monitor the real data as it comes in or set up the experiment but run the first increment a number of times using <tt>array('nt',200,1,0)</tt> to see if the conditioning has been successful.

	== '''cryo_noisetest Macro Procedure (Lower Power, Longer Time)''' ==		== '''cryo_noisetest Macro Procedure (Lower Power, Longer Time)''' ==

	This is an rf decoupling coil conditioning procedure that also quantifies the noise profile of a Cold Probe. The macro runs forever by cycling between periods of prolonged pulsing and testing.		This is an rf decoupling coil conditioning procedure that also quantifies the noise profile of a Cold Probe. The macro runs forever by cycling between periods of prolonged pulsing and testing.

	# Remove the sample from the magnet.		#Remove the sample from the magnet.
	# Ensure that the probe parameter is set to a valid probefile name.		#Ensure that the probe parameter is set to a valid probefile name.
	# Enter <tt>cryo_noisetest</tt>. Enter the number of minutes that the decoupler coil conditioning will run before another quantitative test is done. Add five minutes (for the quantitative testing) to the number you entered to get a total recycle time. For example, 40 will run the conditioning for forty minutes followed by five minutes of tests and then start over. Results are printed out at the end of the tests. Reduce the number for more testing (per unit time), increase it for more.		#Enter <tt>cryo_noisetest</tt>. Enter the number of minutes that the decoupler coil conditioning will run before another quantitative test is done. Add five minutes (for the quantitative testing) to the number you entered to get a total recycle time. For example, 40 will run the conditioning for forty minutes followed by five minutes of tests and then start over. Results are printed out at the end of the tests. Reduce the number for more testing (per unit time), increase it for more.
	# Enter desired number (use 40 during the initial installation of the probe). The procedure starts and a quantitative test is done straight away as a baseline measurement. Four mini-tests are done consecutively; two carbon and two nitrogen, if more than 2 channels are present. For each nucleus CW and WALTZ decoupling modes are selected. An array of <tt>dpwr</tt> and <tt>dpwr2</tt> is done for each nucleus and for each decoupling mode. The noise is estimated from each spectrum and saved in two date-stamped text files in the local users <tt>vnmrsys/data/testlib</tt> directory and also plotted out in graphical form.		#Enter desired number (use 40 during the initial installation of the probe). The procedure starts and a quantitative test is done straight away as a baseline measurement. Four mini-tests are done consecutively; two carbon and two nitrogen, if more than 2 channels are present. For each nucleus CW and WALTZ decoupling modes are selected. An array of <tt>dpwr</tt> and <tt>dpwr2</tt> is done for each nucleus and for each decoupling mode. The noise is estimated from each spectrum and saved in two date-stamped text files in the local users <tt>vnmrsys/data/testlib</tt> directory and also plotted out in graphical form.
	# Enter <tt>aa</tt> or <tt>halt</tt> to stop the acquisition(s) and procedure.		#Enter <tt>aa</tt> or <tt>halt</tt> to stop the acquisition(s) and procedure.

	-- Main.AlexEletski - 07 Mar 2008		-- Main.AlexEletski - 07 Mar 2008