https://nesgwiki.chem.buffalo.edu/index.php?action=history&feed=atom&title=HNCACB%2FCBCA%28CO%29NH 2026-04-19T17:31:00Z Revision history for this page on the wiki MediaWiki 1.38.2 https://nesgwiki.chem.buffalo.edu/index.php?title=HNCACB/CBCA(CO)NH&diff=2498&oldid=prev 2009-11-30T21:41:40Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 21:41, 30 November 2009</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== '''Analysis of the HNNCACB/CACB(CO)NHN'''  ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== '''Analysis of the HNNCACB/CACB(CO)NHN'''  ===</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>In case of NMR data were collected with non-GFT NMR experiments such as HNNCACB/CACß(CO)NHN for backbone assignment, one can treat HNNCACB/CACB(CO)NHN the same as previously described (4,3)GFT HNNCABCA/CABCA(CO)NHN analysis. Simply do the same things as described in analysis of the (4,3)D GFT HNNCABCA and CABCA(CO)NHN spectra: &lt;br&gt;  </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>In case of NMR data were collected with non-GFT NMR experiments such as HNNCACB/CACß(CO)NHN for backbone assignment, one can treat HNNCACB/CACB(CO)NHN the same as previously described <ins style="font-weight: bold; text-decoration: none;">[[HNCACAB/CABCA(CO)NH|</ins>(4,3)GFT HNNCABCA/CABCA(CO)NHN<ins style="font-weight: bold; text-decoration: none;">]] </ins>analysis. Simply do the same things as described in analysis of the <ins style="font-weight: bold; text-decoration: none;">[[HNCACAB/CABCA(CO)NH|</ins>(4,3)D GFT HNNCABCA and CABCA(CO)NHN<ins style="font-weight: bold; text-decoration: none;">]] </ins>spectra: &lt;br&gt;  </div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>#<del style="font-weight: bold; text-decoration: none;">Simulating </del>the intial peak lists<del style="font-weight: bold; text-decoration: none;">; </del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>#<ins style="font-weight: bold; text-decoration: none;">Simulate </ins>the intial peak lists  </div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>#<del style="font-weight: bold; text-decoration: none;">Adjusting </del>peak <del style="font-weight: bold; text-decoration: none;">position </del>in strips <del style="font-weight: bold; text-decoration: none;">of all residues with </del>XEASY<del style="font-weight: bold; text-decoration: none;">; </del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>#<ins style="font-weight: bold; text-decoration: none;">Adjust the </ins>peak <ins style="font-weight: bold; text-decoration: none;">positions </ins>in strips <ins style="font-weight: bold; text-decoration: none;">using [[</ins>XEASY<ins style="font-weight: bold; text-decoration: none;">|XEASY]] </ins></div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>#<del style="font-weight: bold; text-decoration: none;">Simulating </del>AutoAssign input files and do the <del style="font-weight: bold; text-decoration: none;">initially </del>backbone assignment <del style="font-weight: bold; text-decoration: none;">by </del>AutoAssign<del style="font-weight: bold; text-decoration: none;">; </del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>#<ins style="font-weight: bold; text-decoration: none;">Simulate [[</ins>AutoAssign<ins style="font-weight: bold; text-decoration: none;">|AutoAssign]] </ins>input files and do the <ins style="font-weight: bold; text-decoration: none;">initial </ins>backbone assignment <ins style="font-weight: bold; text-decoration: none;">using [[</ins>AutoAssign<ins style="font-weight: bold; text-decoration: none;">|AutoAssign]] </ins></div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>#Maually <del style="font-weight: bold; text-decoration: none;">checking </del>results from AutoAssign and <del style="font-weight: bold; text-decoration: none;">completing </del>the assignment<del style="font-weight: bold; text-decoration: none;">.</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>#Maually <ins style="font-weight: bold; text-decoration: none;">check </ins>results from <ins style="font-weight: bold; text-decoration: none;">[[AutoAssign|</ins>AutoAssign<ins style="font-weight: bold; text-decoration: none;">]] </ins>and <ins style="font-weight: bold; text-decoration: none;">complete </ins>the assignment</div></td></tr> </table>

Jlmills https://nesgwiki.chem.buffalo.edu/index.php?title=HNCACB/CBCA(CO)NH&diff=1774&oldid=prev 2009-11-11T22:24:36Z

<p>Created page with &#039;=== &#039;&#039;&#039;Analysis of the HNNCACB/CACB(CO)NHN&#039;&#039;&#039; === In case of NMR data were collected with non-GFT NMR experiments such as HNNCACB/CACß(CO)NHN for backbone assignment, one can …&#039;</p> <p><b>New page</b></p><div>=== '''Analysis of the HNNCACB/CACB(CO)NHN''' ===<br /> <br /> In case of NMR data were collected with non-GFT NMR experiments such as HNNCACB/CACß(CO)NHN for backbone assignment, one can treat HNNCACB/CACB(CO)NHN the same as previously described (4,3)GFT HNNCABCA/CABCA(CO)NHN analysis. Simply do the same things as described in analysis of the (4,3)D GFT HNNCABCA and CABCA(CO)NHN spectra: &lt;br&gt; <br /> <br /> #Simulating the intial peak lists; <br /> #Adjusting peak position in strips of all residues with XEASY; <br /> #Simulating AutoAssign input files and do the initially backbone assignment by AutoAssign; <br /> #Maually checking results from AutoAssign and completing the assignment.</div>

Agutmana