Inserting NMR Sample - Revision history

Aringh at 16:08, 9 December 2009

2009-12-09T16:08:00Z

Aringh at 22:35, 8 December 2009

2009-12-08T22:35:04Z

← Older revision		Revision as of 22:35, 8 December 2009
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	== Adjusting sample depth with a sample gauge ==		== Adjusting sample depth with a sample gauge ==

	[[Image:Gauge sch.jpg\|right\|100px]]First, the NMR tube ~~has~~ to be inserted into a spinner turbine. For best results, NMR tubes should be positioned in the spinner turbine so that the sample volume will be centered in the NMR probe. This can be done with the help of a sample gauge, which usually has a center line and rectangular markings to indicate the probe geometry.<br>		[[Image:Gauge sch.jpg\|right\|100px]]First, the NMR tube is needed to be inserted into a spinner turbine. For best results, NMR tubes should be positioned in the spinner turbine so that the sample volume will be centered in the NMR probe. This can be done with the help of a sample gauge, which usually has a center line and rectangular markings to indicate the probe geometry.<br>

	A sample gauge also has an adjustable bottom, which can be adjusted to allow quick posistioning of a series of identical samples. Setting a sample tube too low may cause it to break, as well as contaminate and damage the probe!		A sample gauge also has an adjustable bottom, which can be adjusted to allow quick posistioning of a series of identical samples. Setting a sample tube too low may cause it to break, as well as contaminate and damage the probe!
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	http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199931300b.pdf		http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199931300b.pdf

			<br>

			== Working with Bruker Instruments ==

	~~== Working with Bruker Instruments ==~~		As with Varians, the sample tube is loaded into a spinner and the depth is adjusted using a depth gauge.  The base of the depth gauge should be set to the minimum sample tube depth allowed by the probe in use.  DO NOT adjust this level.  The minimum volume depth for most Bruker probes is normally smaller than that for Varians, as a result it is important to use Shigemi tubes suitable for use with Bruker probes (they will have a shorter glass base).

	As with Varians, the sample tube is loaded into a spinner and the depth is adjusted using a depth gauge.  The base of the depth gauge should be set to the minimum sample tube depth allowed by the probe in use.  DO NOT adjust this level.  The minimum volume depth for most Bruker probes is normally smaller than that for Varians, as a result it is important to use Shigemi tubes suitable for use with Bruker probes (they will have a shorter glass base).


			<br>

	Samples are manually ejected and inserted into the magent using the sample eject/insert button on the BSMS panel; the button is located in the top left corner of the BSMS.  Samples can also be ejected using the command line in Topspin (command, ej).		Samples are manually ejected and inserted into the magent using the sample eject/insert button on the BSMS panel; the button is located in the top left corner of the BSMS.  Samples can also be ejected using the command line in Topspin (command, ej).
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	The procedure for inserting the NMR sample into the magnet is analogous to Varians:		The procedure for inserting the NMR sample into the magnet is analogous to Varians:

	#Eject the current sample.  Pressing the eject button on the BSMS (green light comes on) will initiate an airflow and the current sample will float to the top of the bore.		#Eject the current sample.  Pressing the eject button on the BSMS (green light comes on) will initiate an airflow and the current sample will float to the top of the bore.
	#Replace the current sample with the new sample.		#Replace the current sample with the new sample.
	#Pressing the eject button again on the BSMS (green light turns off) stops the airflow and the sample is slowly lowered into the magnet.		#Pressing the eject button again on the BSMS (green light turns off) stops the airflow and the sample is slowly lowered into the magnet.
	#Look for the sample lock to ensure the sample is properly sitting in the probe.		#Look for the sample lock to ensure the sample is properly sitting in the probe.

Jma at 16:27, 4 December 2009

2009-12-04T16:27:38Z

← Older revision		Revision as of 16:27, 4 December 2009
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	http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199916100c.pdf		http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199916100c.pdf

	http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199931300b.pdf		http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199931300b.pdf



			== Working with Bruker Instruments ==

			As with Varians, the sample tube is loaded into a spinner and the depth is adjusted using a depth gauge.  The base of the depth gauge should be set to the minimum sample tube depth allowed by the probe in use.  DO NOT adjust this level.  The minimum volume depth for most Bruker probes is normally smaller than that for Varians, as a result it is important to use Shigemi tubes suitable for use with Bruker probes (they will have a shorter glass base).



			Samples are manually ejected and inserted into the magent using the sample eject/insert button on the BSMS panel; the button is located in the top left corner of the BSMS.  Samples can also be ejected using the command line in Topspin (command, ej).

			The procedure for inserting the NMR sample into the magnet is analogous to Varians:

			#Eject the current sample.  Pressing the eject button on the BSMS (green light comes on) will initiate an airflow and the current sample will float to the top of the bore.
			#Replace the current sample with the new sample.
			#Pressing the eject button again on the BSMS (green light turns off) stops the airflow and the sample is slowly lowered into the magnet.
			#Look for the sample lock to ensure the sample is properly sitting in the probe.

Alex at 22:43, 3 November 2009

2009-11-03T22:43:58Z

← Older revision		Revision as of 22:43, 3 November 2009
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	== External links ==		== External links ==

	http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199916100c.pdf		http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199916100c.pdf

	[http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199931300b.pdf ~~www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199931300b.pdf]~~		http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199931300b.pdf

Alex at 22:43, 3 November 2009

2009-11-03T22:43:30Z

← Older revision		Revision as of 22:43, 3 November 2009
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	== External links ==		== External links ==

			http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199916100c.pdf

	[http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199931300b.pdf www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199931300b.pdf]		[http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199931300b.pdf www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199931300b.pdf]

Alex at 22:35, 3 November 2009

2009-11-03T22:35:58Z

← Older revision		Revision as of 22:35, 3 November 2009
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	== Adjusting sample depth with a sample gauge ==		== Adjusting sample depth with a sample gauge ==

	[[Image:Gauge sch.jpg\|right\|100px~~\|Shigemi tube~~]]First, the NMR tube has to be inserted into a spinner turbine. For best results, NMR tubes should be positioned in the spinner turbine so that the sample volume will be centered in the NMR probe. This can be done with the help of a sample gauge, which usually has a center line and rectangular markings to indicate the probe geometry.<br>		[[Image:Gauge sch.jpg\|right\|100px]]First, the NMR tube has to be inserted into a spinner turbine. For best results, NMR tubes should be positioned in the spinner turbine so that the sample volume will be centered in the NMR probe. This can be done with the help of a sample gauge, which usually has a center line and rectangular markings to indicate the probe geometry.<br>

	A sample gauge also has an adjustable bottom, which can be adjusted to allow quick posistioning of a series of identical samples. Setting a sample tube too low may cause it to break, as well as contaminate and damage the probe!		A sample gauge also has an adjustable bottom, which can be adjusted to allow quick posistioning of a series of identical samples. Setting a sample tube too low may cause it to break, as well as contaminate and damage the probe!

	It is also important to make sure that the tube sits firmly in the spinner. If your sample slides too easily inside a spinner turbine, it may indicate that its rubber o-rings need replacement.		It is also important to make sure that the tube sits firmly in the spinner. If your sample slides too easily inside a spinner turbine, it may indicate that its rubber o-rings need replacement.

	== Inserting and ejecting ==		== Inserting and ejecting ==

	~~In VNMR~~:		To access the '''Insert''' and '''Eject''' buttons do the following:<br>

	~~#Click~~ on the '''Acqi''' button or type <tt>acqi</tt>		*VNMR - click on the '''Acqi''' button or type <tt>acqi</tt><br>
	~~#Use~~ the '''~~Insert''' and '''Eject''' buttons in the '''Acqi~~''' ~~window~~		*VNMRJ - click on the '''Setup''' tab

	In VNMRJ:		You can also type <tt>insert</tt> or <tt>i</tt> and <tt>eject</tt> or <tt>e</tt> at the command prompt in VNMR or VNMRJ.

	~~#Click on the '''Setup''' tab~~		Sample change procedure
	~~#Uset the '''Insert sample''' and '''Eject sample''' buttons in the middle of the screen~~

	~~You can also type <tt>insert</tt> or <tt>i and eject</tt> or e at the VNMR or VNMRJ prompt.~~		#Eject the current sample first. Even if there is no sample in the magnet you need to use eject to create airflow.
			#Remove the ejected sample.<br>
	~~Procedure~~

	#Eject the current sample first. Even if there is no sample in the magnet you need to use eject to create ~~the~~ airflow.
	#Remove the ejected sample.
	#Verify that you have the airflow to support your sample. Carefully insert the sample in the bore.		#Verify that you have the airflow to support your sample. Carefully insert the sample in the bore.
	#~~Select insert~~ to lower your sample		#Click on Insert to lower your sample.<br>

	~~<br> '''Figure2~~. ~~Acquisition window.'''~~ <br>

	~~[[Image:Acq window.jpg]]~~		Avoid leaving the spectrometer with an open bore for long periods of time, as this increases the risk contaminating the bore with forieign particles. If you need to remove a sample and have nothing else to measure, either insert a standard NMR sample or cover the bore with a dedicated plug.


	~~<br> Do not leave~~ the spectrometer with an open bore for long periods of time. If you ~~have~~ to remove a sample and have nothing else to measure, either insert a standard NMR sample or cover the bore with a dedicated plug.

	<br>		<br>
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	== External links ==		== External links ==

	[http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199931300b.pdf www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199931300b.pdf]		[http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199931300b.pdf www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199931300b.pdf]

	~~<br>~~

	~~-- Main.GaohuaLiu - 24 Jan 2007~~

Alex at 22:23, 3 November 2009

2009-11-03T22:23:52Z

← Older revision		Revision as of 22:23, 3 November 2009
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	== Adjusting sample depth with a sample gauge ==		== Adjusting sample depth with a sample gauge ==

	[[Image:Gauge sch.jpg\|right\|100px\|Shigemi tube]] ~~The optimal volume for NMR samples is 500-600 uL for 5 mm regular~~ NMR ~~tubes and ~300 uL for 5 mm Shigemi tubes. Before the~~ tube ~~is put~~ into ~~the magnet~~, the ~~optimal position of~~ the ~~tube~~ in the ~~sample holder should~~ be ~~measured in~~ the gauge ~~as shown in~~ the ~~figure below~~.<br>		[[Image:Gauge sch.jpg\|right\|100px\|Shigemi tube]]First, the NMR tube has to be inserted into a spinner turbine. For best results, NMR tubes should be positioned in the spinner turbine so that the sample volume will be centered in the NMR probe. This can be done with the help of a sample gauge, which usually has a center line and rectangular markings to indicate the probe geometry.<br>

	~~First~~, ~~the NMR tube has~~ to ~~be inserted into~~ a ~~spinner turbine~~. ~~For best results, NMR tubes should be positioned in the spinner turbine so that the sample volume will be centered in the NMR probe. This should be done with the help of~~ a sample ~~gauge~~, ~~which usually has a center line~~ and ~~rectangular markings to indicate~~ the probe ~~geometry.<br>~~		A sample gauge also has an adjustable bottom, which can be adjusted to allow quick posistioning of a series of identical samples. Setting a sample tube too low may cause it to break, as well as contaminate and damage the probe!

	A sample gauge also has an adjustable bottom, which is typically fixed at a proper level by your NMR facility manager. Never position a tube lower than the bottom of the gauge! Setting a sample tube too low may cause it to break, as well as contaminate and damage the probe!		It is also important to make sure that the tube sits firmly in the spinner. If your sample slides too easily inside a spinner turbine, it may indicate that its rubber o-rings need replacement.

	It is also important to make sure that the tube sits firmly in the spinner. If your sample slides too easily inside a ~~spinned~~, ~~that~~ may indicate that its rubber o-rings need replacement.

	== Inserting and ejecting ==		== Inserting and ejecting ==

Alex at 22:07, 29 October 2009

2009-10-29T22:07:15Z

← Older revision		Revision as of 22:07, 29 October 2009
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	<br> Do not leave the spectrometer with an open bore for long periods of time. If you have to remove a sample and have nothing else to measure, either insert a standard NMR sample or cover the bore with a dedicated plug.		<br> Do not leave the spectrometer with an open bore for long periods of time. If you have to remove a sample and have nothing else to measure, either insert a standard NMR sample or cover the bore with a dedicated plug.

			<br>

			== External links ==

			[http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199931300b.pdf www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199931300b.pdf]

	<br>		<br>

	-- Main.GaohuaLiu - 24 Jan 2007		-- Main.GaohuaLiu - 24 Jan 2007

Alex at 22:05, 29 October 2009

2009-10-29T22:05:37Z

← Older revision		Revision as of 22:05, 29 October 2009
Line 3:		Line 3:
	== Adjusting sample depth with a sample gauge ==		== Adjusting sample depth with a sample gauge ==

	[[Image:Gauge ~~reg~~.jpg~~\|200px~~\|right~~]] [[Image:Gauge sch.jpg~~\|~~200px~~\|~~right~~]] The optimal volume for NMR samples is 500-600 uL for 5 mm regular NMR tubes and ~300 uL for 5 mm Shigemi tubes. Before the tube is put into the magnet, the optimal position of the tube in the sample holder should be measured in the gauge as shown in the figure below.<br>		[[Image:Gauge sch.jpg\|right\|100px\|Shigemi tube]] The optimal volume for NMR samples is 500-600 uL for 5 mm regular NMR tubes and ~300 uL for 5 mm Shigemi tubes. Before the tube is put into the magnet, the optimal position of the tube in the sample holder should be measured in the gauge as shown in the figure below.<br>

	First, the NMR tube has to be inserted into a spinner turbine. For best results, NMR tubes should be positioned in the spinner turbine so that the sample volume will be centered in the NMR probe. This should be done with the help of a sample gauge, which usually has a center line and rectangular markings to indicate the probe geometry.<br>		First, the NMR tube has to be inserted into a spinner turbine. For best results, NMR tubes should be positioned in the spinner turbine so that the sample volume will be centered in the NMR probe. This should be done with the help of a sample gauge, which usually has a center line and rectangular markings to indicate the probe geometry.<br>
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	A sample gauge also has an adjustable bottom, which is typically fixed at a proper level by your NMR facility manager. Never position a tube lower than the bottom of the gauge! Setting a sample tube too low may cause it to break, as well as contaminate and damage the probe!		A sample gauge also has an adjustable bottom, which is typically fixed at a proper level by your NMR facility manager. Never position a tube lower than the bottom of the gauge! Setting a sample tube too low may cause it to break, as well as contaminate and damage the probe!

	It is also important to make sure that the tube sits firmly in the spinner. If your sample slides too easily inside a spinned, that may indicate that its rubber o-rings need replacement.		It is also important to make sure that the tube sits firmly in the spinner. If your sample slides too easily inside a spinned, that may indicate that its rubber o-rings need replacement.

	== Inserting and ejecting ==		== Inserting and ejecting ==

Alex at 22:02, 29 October 2009

2009-10-29T22:02:41Z

← Older revision		Revision as of 22:02, 29 October 2009
Line 3:		Line 3:
	== Adjusting sample depth with a sample gauge ==		== Adjusting sample depth with a sample gauge ==

	[[Image:Gauge reg.jpg\|~~frame~~\|right]] [[Image:Gauge sch.jpg\|~~frame~~\|right]] The optimal volume for NMR samples is 500-600 uL for 5 mm regular NMR tubes and ~300 uL for 5 mm Shigemi tubes. Before the tube is put into the magnet, the optimal position of the tube in the sample holder should be measured in the gauge as shown in the figure below.<br>		[[Image:Gauge reg.jpg\|200px\|right]] [[Image:Gauge sch.jpg\|200px\|right]] The optimal volume for NMR samples is 500-600 uL for 5 mm regular NMR tubes and ~300 uL for 5 mm Shigemi tubes. Before the tube is put into the magnet, the optimal position of the tube in the sample holder should be measured in the gauge as shown in the figure below.<br>

	First, the NMR tube has to be inserted into a spinner turbine. For best results, NMR tubes should be positioned in the spinner turbine so that the sample volume will be centered in the NMR probe. This should be done with the help of a sample gauge, which usually has a center line and rectangular markings to indicate the probe geometry.<br>		First, the NMR tube has to be inserted into a spinner turbine. For best results, NMR tubes should be positioned in the spinner turbine so that the sample volume will be centered in the NMR probe. This should be done with the help of a sample gauge, which usually has a center line and rectangular markings to indicate the probe geometry.<br>

← Older revision		Revision as of 16:08, 9 December 2009
Line 3:		Line 3:
	== Adjusting sample depth with a sample gauge ==		== Adjusting sample depth with a sample gauge ==

	[[Image:Gauge sch.jpg\|right\|100px]]First, the NMR tube is needed to be inserted into a spinner turbine. For best results, NMR tubes should be positioned in the spinner turbine so that the sample ~~volume will be~~ centered in the NMR probe. This can be done with the help of a sample gauge, which usually has a center line and rectangular markings to indicate the probe geometry.<br>		[[Image:Gauge sch.jpg\|right\|100px]]First, the NMR tube is needed to be inserted into a spinner turbine. For best results, NMR tubes should be positioned in the spinner turbine in such a way that the sample remains centered in the r.f. coil region inside the NMR probe. This can be done with the help of a sample gauge, which usually has a center line and rectangular markings to indicate the probe geometry.<br>

	A sample gauge also has an adjustable bottom, which can be adjusted to allow quick ~~posistioning~~ of a series of identical samples. Setting a sample tube too low may cause it to break, as well as contaminate and damage the probe!		A sample gauge also has an adjustable bottom, which can be adjusted to allow quick positioning of a series of identical samples. Setting a sample tube too low may cause it to break, as well as contaminate and damage the probe!

	It is also important to make sure that the tube sits firmly in the spinner. If your sample slides too easily inside a spinner turbine, it may indicate that ~~its~~ rubber o-rings need replacement.		It is also important to make sure that the tube sits firmly in the spinner. If your sample slides too easily inside a spinner turbine, it may indicate that the rubber o-rings of the spinner need replacement.

	== Inserting and ejecting ==		== Inserting and ejecting ==
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	Sample change procedure		Sample change procedure

	#Eject the current sample first. Even if there is no sample in the magnet you need to use eject to create airflow.		#Eject the current sample first. Even if there is no sample in the magnet you need to use eject to create the upward thrust using the airflow.
	#Remove the ejected sample.<br>		#Remove the ejected sample.<br>
	#Verify that you have the airflow to support your sample. ~~Carefully insert~~ the sample ~~in the bore~~.		#Place the sample in the spinner in the bore. Verify that you have the airflow to support your sample while still holding the sample. Leave the sample.
	#Click on Insert to lower your sample.<br>		#Click on Insert to lower your sample.<br>

	Avoid leaving the spectrometer with an open bore for long periods of time, as this increases the risk ~~contaminating~~ the bore with ~~forieign~~ particles. If you need to remove a sample and have nothing else to measure, either insert a standard NMR sample or cover the bore with a dedicated plug.		Avoid leaving the spectrometer with an open bore for long periods of time, as this increases the risk of contamination of the bore with foreign particles. If you need to remove a sample and have nothing else to measure, either insert a standard NMR sample or cover the bore with a dedicated plug.

	<br>		<br>
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	<br>		<br>

	Samples are manually ejected and inserted into the ~~magent~~ using the sample eject/insert button on the BSMS panel; the button is located in the top left corner of the BSMS.  Samples can also be ejected using the command line in Topspin (command, ej).		Samples are manually ejected and inserted into the magnet using the sample eject/insert button on the BSMS panel; the button is located in the top left corner of the BSMS.  Samples can also be ejected using the command line in Topspin (command, ej).

	The procedure for inserting the NMR sample into the magnet is analogous to Varians:		The procedure for inserting the NMR sample into the magnet is analogous to Varians:

	#Eject the current sample.  Pressing the eject button on the BSMS (green light comes on) will initiate an airflow and the current sample will float to the top of the bore.		#Eject the current sample.  Pressing the eject button on the BSMS (green light comes on) will initiate an upward airflow and the current sample will float to the top of the bore.
	#Replace the current sample with the new sample.		#Replace the current sample with the new sample.
	#Pressing the eject button again on the BSMS (green light turns off) stops the airflow and the sample is slowly lowered into the magnet.		#Pressing the eject button again on the BSMS (green light turns off) stops the airflow and the sample is slowly lowered into the magnet.
	#Look for the sample lock to ensure the sample is properly sitting in the probe.		#Look for the sample lock to ensure the sample is properly sitting in the probe.