Resonance Assignment/Principles and concepts

Introduction

This section is intended to introduce a few definitions and concepts in resonance assignment protocols. For in depth description of the process see e.g. Kurt Wütrich's book NMR of Proteins and Nucleic Acids (Wiley, 1986) and John Cavangh's et al. textbook Protein NMR Spectroscopy. Principles and Practice (2^nd edition, Elsevier, 2007). 

Stable isotope labeling schemes

Through the NESG consortium, the most prevalent isotope labeling schemes are as follows:

• 100% ¹⁵N, 100%¹³C-labeled (or doubly-labeled) samples are the main category, to which the majority of the information on this site applies. They are used for complete resonance assignments and structure calculation.
  
• 100% ¹⁵N-labeled samples are used for screening with 15N-HSQC. They can also find limited use in collecting RDC-type data.
  
• 100% ¹⁵N, 5-7% ¹³C-labeled samples are used to obtain stereospecific assignment of Val and Leu side chain methyl groups, usually important for proper packing of hydrophobic core.
• 100% 14N, 100% 12C (or unlabeled) or alternatively natural abundance samples can be used in 50%-50% mixtures for homodimer structure determination.
  

In addition to these labeling schemes, one can find it useful, especially larger proteins to have selectively labeled samples, such as SAIL NMR (http://www.sailnmr.org/). To reduce signal broadening due to spin-spin relaxation, it may be advantageous to deuterate the protein to a certain level.

NMR experiments

This section describes the types of connectivities that can be established between nuclei by given experiments. For specifics and experimental setup, please refer to the NMR Data Collection section of this site.

Through bond

Through-bond experiments correlate nuclei connected by a limited number of chemical bonds.

• ¹⁵N-HSQC - correlates a ¹⁵N nucleus and a 1H directly attached to it. Mainly used to identify backbone amide groups.
  
• ¹³C-HSQC - correlates a ¹³C nucleus and a 1H directly attached to it. Can cover aliphatic and/or aromatic range of ¹³C chemical shifts
  
  • Constant time version enhances resolution. Peak sign gives information on the number of ¹³C neighbors a given ¹³C nucleus has
• HNCO, 3D - correlates a backbone amide with the C' of preceding residue or a Cγ/Cδ of Asn/Gln with NH₂ of the side chain
• HNCA, 3D - correlates a backbone amide with the Cα of the same and preceding residues
• HNCACB, 3D - correlates a backbone amide with the Cα and Cβ of the same and preceding residues. Usually has Cβ and Cα peaks of opposite signs
• CBCA(CO)NH, 3D - correlates a backbone amide with the Cα and Cβ of the preceding residue. Together with HNCACB, HNCA and HNCO it is used to assign the backbone chemical shifts.
  
• HBHA(CBCACO)NH, 3D - correlates a backbone amide with the Hα and Hβ resonances of the preceding residue. Used together with HCCH-type experiments and ¹³C-HSQC to assign the side chain resonances.
  
• H(C)CH-COSY, 3D - correlates an aliphatic C-H pair with an adjacent aliphatic ¹H of the same side chain. Closely related experiment is H(C)CH-TOCSY, 3D, which correlates an aliphatic C-H pair with all aliphatic ¹H nuclei of the same side chain.
• (H)CCH-COSY, 3D, and (H)CCH-TOCSY, 3D, are very similar to above, except the third correlated nucleus is ¹³C
• All HCCH-type spectra can also be taylored for the aromatic region of 13C range. Correlations would be detected only within an individual aromatic ring.
• (HB)CB(CGCD)HD and (HB)CB(CGCDCE)HE, 2D - correlate Hδ and Hε aromatic protons with Cβ of the same residue. Useful for assigning chemical shifts of nuclei in aromatic rings.

• Long range 15N-HSQC, 2D - correlates Hδ2 and Hε1 protons (attached to ¹²C or ¹³C nuclei!) on His rings with Nδ1 and Nε2 nuclei. This experiment s useful for assigning Hε1 and Cε1 nuclei of His rings as well as to determine the protonation state of the ring.

Through space

Through-space experiments, as the name implies, correlate pairs of nuclei close in space. Many such nuclei would belong to the same or adjacent residues and these experiments can thus supplement the information obtained from through-bond spectra. The usual distance limit to observe such a correlation is ~5Â.

• ¹⁵N-edited NOESY-HSQC, 3D - correlates all protons close to the ¹H of an amide group with both nuclei of the amide
• ¹³C-edited NOESY-HSQC, 3D - correlates all protons close to the ¹H of a C-H moiety with both nuclei of the moiety. Can replace an H(C)CH-TOCSY experiment in certain cases. 
  • The above spectrum can be separated into aliphatic and aromatic regions of the ¹³C range.
• Simultaneous ¹³C,¹⁵N-edited NOESY-HSQC, 3D - as the name implies, this spectrum combines the above two experiments (¹⁵N and ¹³C).

These NOESY-type spectra are very useful in confirming the sequence specific backbone assignments and in connecting aromatic rings to their proper side chains. For certain automatic methods (e.g. ABACUS) they contain all necessary sequencial information and can thus completely replace HNCACB or similar spectra.

Spin systems

Definition

A spin system in the broadest sense of the word is a set of nuclei connected by chemical bonds. While for small molecules this is a very useful definition, for proteins it becomes unwieldy as the whole protein would thus be just one spin system. In practice, in protein NMR, a spin system is usually defined as an amino acid residue (AA-fragment) - N, HN, Cα, Hα, C', and all nuclei of the side chain. 

Sometimes it may be more convenient to define apeptide-bond fragment (PB-fragment) spin system, which would contain N and HN of the following amino acid, instead of the same. See figure.

Occasionaly, an aromatic ring may be treated as a separate spin system from the rest of the same side chain.