Common NMR experiment sets

Szyperski group

The following experiments are recorded for NC samples

Common experiments

• 1D
• 1D 15N T1
• 1D 15N T2
• 2D [15N,1H]-HSQC
• 2D [15N,1H]-HSQC with wide spectral width in 15N (optional)
  
• 2D [13C,1H]-HSQC aliphatic
  
• 2D [13C,1H]-HSQC aromatic (optional)
  
• 2D [13C,1H]-CT-HSQC aliphatic
• 2D [13C,1H]-CT-HSQC aromatic
• 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
  
• 3D HN(CA)CO (optional)
• 3D (H)CCH-TOCSY
• 2D HE(CECDCG)CB (optional)
• 3D 15N/13Cali/13Caro-resolved [1H,1H]-NOESY

GFT protocol

• (3,2)D GFT HNNCO or 3D HNCO
  
• (4,3)D GFT CABCA(CO)NH
  
• (4,3)D GFT HNCABCA
  
• (4,3)D GFT HABCAB(CO)NH
  
• (4,3)D GFT HCCH aliphatic
  
• (4,3)D GFT HCCH-COSY aromatic

Conventional protocol

• 3D HNCO
• 3D HN(CA)CO
• 3D CBCA(CO)NH
• 3D HNCACB
• 3D HBHA(CO)NH
• (H)CCH-COSY aliphatic
• (H)CCH-COSY aromatic

Fractionally 13C-labeled samples

• 2D [13C,1H]-CT-HSQC, 28ms delay - optimized for methyl groups
  
• 2D [13C,1H]-CT-HSQC, 56ms delay (optional)
  
• 2D [13C,1H]-CT-HSQC, 42ms delay (optional)
• 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional - can also be run for the NC sample)
  

Arrowsmith group

Data acquisition is taylored for ABACUS approach for resonance assignment. Most 3D spectra collected with Non-uniform sampling and processed with Multidimensional decomposition,

• 1D presaturation
• 1D 15N T1
• 1D 15N T2
• 2D [15N,1H]-HSQC
• 2D [13C,1H]-HSQC aliphatic
• 2D [13C,1H]-HSQC aromatic
• 2D [13C,1H]-CT-HSQC aliphatic
• 2D [13C,1H]-CT-HSQC aromatic (optional)
• 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
• 2D HE(CECDCG)CB (optional)

{!NH-rooted !!CH-rooted !!Aromatic |- |

• 3D 15N-NOESY-HSQC
• 3D HNCO
• 3D HNCA
• 3D CBCA(CO)NH
• 3D HBHA(CO)NH

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• 3D (H)CCH-TOCSY
• 3D H(C)CH-TOCSY
• 3D 13C-NOESY-HSQC

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• 3D (H)CCH-TOCSY (optional)
• 3D H(C)CH-TOCSY (optional)
• 3D 13C-NOESY-HSQC