Common NMR experiment sets: Difference between revisions
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*1D presaturation | *1D presaturation | ||
*1D 15N T1 | *1D 15N T1 | ||
*1D 15N T2 | *1D 15N T2 | ||
*2D [15N,1H]-HSQC | *2D [15N,1H]-HSQC | ||
*2D [13C,1H]-HSQC aliphatic | *2D [13C,1H]-HSQC aliphatic | ||
| Line 66: | Line 66: | ||
*2D [13C,1H]-CT-HSQC aliphatic | *2D [13C,1H]-CT-HSQC aliphatic | ||
*2D [13C,1H]-CT-HSQC aromatic (optional) | *2D [13C,1H]-CT-HSQC aromatic (optional) | ||
*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional) | *2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional) | ||
*2D HE(CECDCG)CB (optional) | *2D HE(CECDCG)CB (optional) | ||
{!NH-rooted | {!NH-rooted !!CH-rooted !!Aromatic | ||
!!CH-rooted | |- | ||
!!Aromatic | | | ||
|- | *3D 15N-NOESY-HSQC | ||
| | |||
*3D 15N-NOESY-HSQC | |||
*3D HNCO | *3D HNCO | ||
*3D HNCA | *3D HNCA | ||
*3D CBCA(CO)NH | *3D CBCA(CO)NH | ||
*3D HBHA(CO)NH | *3D HBHA(CO)NH | ||
|| | || | ||
*3D (H)CCH-TOCSY | *3D (H)CCH-TOCSY | ||
*3D H(C)CH-TOCSY | *3D H(C)CH-TOCSY | ||
*3D 13C-NOESY-HSQC | *3D 13C-NOESY-HSQC | ||
|| | || | ||
*3D (H)CCH-TOCSY (optional) | *3D (H)CCH-TOCSY (optional) | ||
*3D H(C)CH-TOCSY (optional) | *3D H(C)CH-TOCSY (optional) | ||
*3D 13C-NOESY-HSQC | *3D 13C-NOESY-HSQC | ||
|} | |||
Revision as of 21:39, 23 December 2009
Szyperski group
The following experiments are recorded for NC samples
Common experiments
- 1D
- 1D 15N T1
- 1D 15N T2
- 2D [15N,1H]-HSQC
- 2D [15N,1H]-HSQC with wide spectral width in 15N (optional)
- 2D [13C,1H]-HSQC aliphatic
- 2D [13C,1H]-HSQC aromatic (optional)
- 2D [13C,1H]-CT-HSQC aliphatic
- 2D [13C,1H]-CT-HSQC aromatic
- 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
- 3D HN(CA)CO (optional)
- 3D (H)CCH-TOCSY
- 2D HE(CECDCG)CB (optional)
- 3D 15N/13Cali/13Caro-resolved [1H,1H]-NOESY
GFT protocol
- (3,2)D GFT HNNCO or 3D HNCO
- (4,3)D GFT CABCA(CO)NH
- (4,3)D GFT HNCABCA
- (4,3)D GFT HABCAB(CO)NH
- (4,3)D GFT HCCH aliphatic
- (4,3)D GFT HCCH-COSY aromatic
Conventional protocol
- 3D HNCO
- 3D HN(CA)CO
- 3D CBCA(CO)NH
- 3D HNCACB
- 3D HBHA(CO)NH
- (H)CCH-COSY aliphatic
- (H)CCH-COSY aromatic
Fractionally 13C-labeled samples
- 2D [13C,1H]-CT-HSQC, 28ms delay - optimized for methyl groups
- 2D [13C,1H]-CT-HSQC, 56ms delay (optional)
- 2D [13C,1H]-CT-HSQC, 42ms delay (optional)
- 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional - can also be run for the NC sample)
Arrowsmith group
Data acquisition is taylored for ABACUS approach for resonance assignment. Most 3D spectra collected with Non-uniform sampling and processed with Multidimensional decomposition,
- 1D presaturation
- 1D 15N T1
- 1D 15N T2
- 2D [15N,1H]-HSQC
- 2D [13C,1H]-HSQC aliphatic
- 2D [13C,1H]-HSQC aromatic
- 2D [13C,1H]-CT-HSQC aliphatic
- 2D [13C,1H]-CT-HSQC aromatic (optional)
- 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
- 2D HE(CECDCG)CB (optional)
{!NH-rooted !!CH-rooted !!Aromatic |- |
- 3D 15N-NOESY-HSQC
- 3D HNCO
- 3D HNCA
- 3D CBCA(CO)NH
- 3D HBHA(CO)NH
||
- 3D (H)CCH-TOCSY
- 3D H(C)CH-TOCSY
- 3D 13C-NOESY-HSQC
||
- 3D (H)CCH-TOCSY (optional)
- 3D H(C)CH-TOCSY (optional)
- 3D 13C-NOESY-HSQC
|}