Common NMR experiment sets: Difference between revisions
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Data acquisition is taylored for [[Abacus|ABACUS]] approach for resonance assignment. Most 3D spectra are collected with [[Setting up non-uniformly sampled spectra|Non-uniform sampling]] and processed with [[Processing non-uniformly sampled spectra with Multidimensional Decomposition|Multidimensional decomposition]], | Data acquisition is taylored for [[Abacus|ABACUS]] approach for resonance assignment. Most 3D spectra are collected with [[Setting up non-uniformly sampled spectra|Non-uniform sampling]] and processed with [[Processing non-uniformly sampled spectra with Multidimensional Decomposition|Multidimensional decomposition]], | ||
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{| | {| | ||
|- | |- | ||
! NH-rooted | ! NH-rooted | ||
! CH-rooted | ! CH-rooted (aliphatic) | ||
! | ! CH-rooted (aromatic) | ||
! Other | ! Other | ||
|- valign="top" | |- valign="top" | ||
| Line 77: | Line 78: | ||
| | | | ||
*2D [13C,1H]-HSQC | *2D [13C,1H]-HSQC | ||
*2D [13C,1H]-CT-HSQC | *2D [13C,1H]-CT-HSQC | ||
*3D 13C-NOESY-HSQC | *3D 13C-NOESY-HSQC | ||
*3D (H)CCH-TOCSY | *3D (H)CCH-TOCSY | ||
*3D H(C)CH-TOCSY | *3D H(C)CH-TOCSY | ||
| | | | ||
*2D [13C,1H]-HSQC | *2D [13C,1H]-HSQC | ||
*2D [13C,1H]-CT-HSQC | *2D [13C,1H]-CT-HSQC (optional) | ||
*3D 13C-NOESY-HSQC | *3D 13C-NOESY-HSQC | ||
*3D (H)CCH-TOCSY (optional) | *3D (H)CCH-TOCSY (optional) | ||
*3D H(C)CH-TOCSY (optional) | *3D H(C)CH-TOCSY (optional) | ||
| | |||
| | |||
*1D presaturation | *1D presaturation | ||
*1D 15N T1 | *1D 15N T1 | ||
*1D 15N T2 | *1D 15N T2 | ||
*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional) | *2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional) | ||
*2D HE(CECDCG)CB (optional) | *2D HE(CECDCG)CB (optional) | ||
*2D HD(CDCG)CB (optional) | |||
|} | |} | ||
Revision as of 21:52, 23 December 2009
Szyperski group
The following experiments are recorded for NC samples
Common experiments
- 1D
- 1D 15N T1
- 1D 15N T2
- 2D [15N,1H]-HSQC
- 2D [15N,1H]-HSQC with wide spectral width in 15N (optional)
- 2D [13C,1H]-HSQC aliphatic
- 2D [13C,1H]-HSQC aromatic (optional)
- 2D [13C,1H]-CT-HSQC aliphatic
- 2D [13C,1H]-CT-HSQC aromatic
- 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
- 3D HN(CA)CO (optional)
- 3D (H)CCH-TOCSY
- 2D HE(CECDCG)CB (optional)
- 3D 15N/13Cali/13Caro-resolved [1H,1H]-NOESY
GFT protocol
- (3,2)D GFT HNNCO or 3D HNCO
- (4,3)D GFT CABCA(CO)NH
- (4,3)D GFT HNCABCA
- (4,3)D GFT HABCAB(CO)NH
- (4,3)D GFT HCCH aliphatic
- (4,3)D GFT HCCH-COSY aromatic
Conventional protocol
- 3D HNCO
- 3D HN(CA)CO
- 3D CBCA(CO)NH
- 3D HNCACB
- 3D HBHA(CO)NH
- (H)CCH-COSY aliphatic
- (H)CCH-COSY aromatic
Fractionally 13C-labeled samples
- 2D [13C,1H]-CT-HSQC, 28ms delay - optimized for methyl groups
- 2D [13C,1H]-CT-HSQC, 56ms delay (optional)
- 2D [13C,1H]-CT-HSQC, 42ms delay (optional)
- 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional - can also be run for the NC sample)
Arrowsmith group
Data acquisition is taylored for ABACUS approach for resonance assignment. Most 3D spectra are collected with Non-uniform sampling and processed with Multidimensional decomposition,
| NH-rooted | CH-rooted (aliphatic) | CH-rooted (aromatic) | Other |
|---|---|---|---|
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