Resonance Assignment/CARA: Difference between revisions

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== Loading a New Template  ==
The topics below provide an overview of resonance assignment of 15N,13C-labeled proteins using CARA. For more information and tutorials please consult [http://www.nmr.ch CARA web site].


To start a new structure determination project in CARA you need to load a template. A template is a CARA repository without project that contains definitions for residue types, spectrum type and LUA scripts. You can either load the default template from the [http://www.cara.ethz.ch/Wiki/TemplatesPage templates page] of the official CARA web-site, or extract a template from an existing CARA repository.
*[[CARA Introduction|Introduction]]
*[[CARA vs Xeasy|Differences from XEASY]] 
*[[CARA Scopes|Scopes - Displaying NMR Spectra]]


For detailed instructions see: http://www.cara.ethz.ch/Wiki/ImportingTemplate


<br>[[Image:CARA New Template Dialogue.png]]  
*[[Resonance Assignment/CARA/Starting a new project|Starting a new project]]
*[[Resonance Assignment/CARA/Backbone assignment|Backbone resonance assignment]]
*Side Chain Assignment
**[[Aliphatic Side Chain Assignment with CARA|Aliphatic side-chain assignment]]
**[[Aromatic Side Chain Assignment with CARA|Aromatic side-chain assignment]]
**[[Amide Side Chain Assignment with CARA|Amide side-chain assignment]]
*[[SSAFromFractional13CLabeledSample|Stereospecific Assignment of Leu and Val Isopropyl Groups]]
*[[Creating NOESY peaklists with CARA|Creating NOESY peaklists]]


CARA Template for GFT spectra [[Media:BoR54_template.cara|BoR54_template.cara]]: Latest GFT template for BoR54 project


Key modifications are:
Also see [[Resonance_Assignment/CARA/Backbone_assignment_GFT|backbone assignment with GFT spectra]]
*Added linker residue definitions to facilitate treatment of homodimers
*Added definitions of (4,3)D HNNCABCA, (4,3)D CABCA(CO)NHN and (4,3)D HABCAB(CO)NHN
*Added attributes for GFT spectra - used by Lua scripts
*LUA scripts
**CreateConsensusPeaklist - consensus peaklist from two loaded peak lists
**CreateSequentialSpinLinks
**ExportSeqToAutostructure - prepare sequence in Autostructure format
**ExportToAutoAssign - generate all files for AutoAssign input
**ExportToCSI - generate CSI input file
**GFT_aroHCCH_calc - small applet too calculate aromatic side-chain spin in (4,3)D HCCH-COSY
**GFT_HCCH_calc - small applet too calculate aliphatic side-chain spin in (4,3)D HCCH-COSY
**GFT_CABCA2CACB - calculate CA and CB from sum and difference shifts
**ImportCyana2xPeakList - import peaklist in CYANA 2.x format (with multiple assignments)
**ImportSparkyPeakList - import peaklist in Sparky format (from AutoStructure)
**ImportFromAutoAssign - read assignments produced by AutoAssign
**etc...
 
 
 
#[[CARA Introduction|Introduction]]
#[[Spin System Identification with CARA|Spin System Identification in 2D 15N-HSQC and 3D HNNCO]]
#[[Backbone Assignment with CARA|Backbone Resonance Assignment]]
#[[HA and HB Assignment with CARA|Assignment of HA and HB Resonances with (4,3)D GFT HABCAB(CO)NHN]]
#Side Chain Assignment
##[[Aliphatic Side Chain Assignment with CARA|Aliphatic side-chain assignment]]
##[[Aromatic Side Chain Assignment with CARA|Aromatic side-chain assignment]]
##[[Amide Side Chain Assignment with CARA|Amide side-chain assignment]]

Latest revision as of 20:13, 8 August 2012

The topics below provide an overview of resonance assignment of 15N,13C-labeled proteins using CARA. For more information and tutorials please consult CARA web site.



Also see backbone assignment with GFT spectra

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