Spin System Identification with CARA: Difference between revisions
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== '''Spin System Identification with CARA''' == | == '''Spin System Identification with CARA''' == | ||
Spin system identification in 2D [15N,1H]-HSQC and 3D HNCO | Spin system identification in 2D [15N,1H]-HSQC and 3D HNCO | ||
# Open a repositiry or create one if it does not exist (see the CARA page). | #Open a repositiry or create one if it does not exist (see the CARA page). | ||
# Open 2D [15N,1H]-HSQC in SynchroScope to determine the region with true peak region and noise level. | #Open 2D [15N,1H]-HSQC in SynchroScope to determine the region with true peak region and noise level. | ||
# Run Lua script '''Pick_2D_Peaks'''. This script should be in the repository, otherwise get it from http://www.cara.ethz.ch/Wiki/AllCaluaScripts . '''Pick_2D_Peaks''' is equivalent to the <tt>in</tt> command in XEASY, but is not interactive. The script will create spin systems and populate them with H and N spins. Note that in CARA spin systems are distinct from residues and can have numbers starting with 1. | #Run Lua script '''Pick_2D_Peaks'''. This script should be in the repository, otherwise get it from http://www.cara.ethz.ch/Wiki/AllCaluaScripts . '''Pick_2D_Peaks''' is equivalent to the <tt>in</tt> command in XEASY, but is not interactive. The script will create spin systems and populate them with H and N spins. Note that in CARA spin systems are distinct from residues and can have numbers starting with 1. | ||
# To delete unwanted spin systems in SynchroScope use '''right click - | #To delete unwanted spin systems in SynchroScope use '''right click -> Delete Systems'''. To pick additional spin systems use '''right click -> Pick System''' | ||
# Open 3D HNCO in the strip panels of SynchroScope by using '''Strips - | #Open 3D HNCO in the strip panels of SynchroScope by using '''Strips -> Select Spectrum''' or '''right click -> Select Spectrum''' | ||
# Select a spin system by left-clicking in HSQC plane or using '''View - | #Select a spin system by left-clicking in HSQC plane or using '''View -> Goto System''' - this updates the strip display. | ||
# In the strip panel set the cursor on a peak and use '''right click - | #In the strip panel set the cursor on a peak and use '''right click -> Pick Label...''' or '''right click -> Pick Spin''' and '''right click -> Label Spin''' to pick C-1. Repeat last two steps for all spin systems. | ||
Also see instructions on how to use SynchroScope here: | Also see instructions on how to use SynchroScope here: http://www.cara.ethz.ch/Wiki/SynchroScope | ||
http://www.cara.ethz.ch/Wiki/SynchroScope | |||
<br> | |||
<br>-- Main.AlexEletski - 06 Jul 2007 | |||
-- Main.AlexEletski - 06 Jul 2007 | |||
Latest revision as of 17:15, 6 November 2009
Spin System Identification with CARA
Spin system identification in 2D [15N,1H]-HSQC and 3D HNCO
- Open a repositiry or create one if it does not exist (see the CARA page).
- Open 2D [15N,1H]-HSQC in SynchroScope to determine the region with true peak region and noise level.
- Run Lua script Pick_2D_Peaks. This script should be in the repository, otherwise get it from http://www.cara.ethz.ch/Wiki/AllCaluaScripts . Pick_2D_Peaks is equivalent to the in command in XEASY, but is not interactive. The script will create spin systems and populate them with H and N spins. Note that in CARA spin systems are distinct from residues and can have numbers starting with 1.
- To delete unwanted spin systems in SynchroScope use right click -> Delete Systems. To pick additional spin systems use right click -> Pick System
- Open 3D HNCO in the strip panels of SynchroScope by using Strips -> Select Spectrum or right click -> Select Spectrum
- Select a spin system by left-clicking in HSQC plane or using View -> Goto System - this updates the strip display.
- In the strip panel set the cursor on a peak and use right click -> Pick Label... or right click -> Pick Spin and right click -> Label Spin to pick C-1. Repeat last two steps for all spin systems.
Also see instructions on how to use SynchroScope here: http://www.cara.ethz.ch/Wiki/SynchroScope
-- Main.AlexEletski - 06 Jul 2007