Protein purification: Difference between revisions

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For each protein, we usually make the following samples:  
For each protein, we usually make the following samples:  


*   100% 15N, 100% 13C uniform labeled sample, for resonance assignment and NOE interpretion.  
*[''U''-<sup>15</sup>N, <sup>13</sup>C]-labeled sample, for resonance assignment and NOE interpretation.  
*&nbsp;&nbsp; 100% 15N, 5% 13C labeled sample, for stereospecific assignment of VAL and LEU isopropyl moieties.
*


For RDC measurement:
A U-<sup>15</sup>N and fractional 5%-<sup>13</sup>C-labeled sample for stereospecific assignment of Val and Leu isopropyl methyl groups based on the method of Neri et. al. (Biochemistry 28, 7510-7516,1989) that we call the NC5 sample.&nbsp; This sample is prepared using 5% ''U''[<sup>1</sup>H,<sup>13</sup>C]-D-glucose and 95% unlabelled glucose in the ''E. coli'' minimal&nbsp; growth media.


*&nbsp;&nbsp; A secondary 100% 15N, 5% 13C labeled sample, for RDC measurement.
<br> The NC5 sample is not [''U''-5%-<sup>13</sup>C,''U''-<sup>15</sup>N] since it is not uniformly 5% -<sup>13</sup>C labeled.&nbsp; The incorporation of <sup>13</sup>C in the methyl groups is biosynthetically directed by the ''E. coli''.&nbsp;  


For each protein that exist as dimer in solution an extra sample may be required in addition to the samples above:  
This sample can be called:<br>


*&nbsp;&nbsp; 1:1 unlabeled and 100% 15N, 100% 13C uniformed labeled mixed sample, for intermolecular NOE interpretation
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; ''<span>U</span>''<span>-<sup>15</sup>N, 5%
biosynthetically directed <sup>13</sup>C (NC5) sample or</span>


&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp; ''<span>U</span>''<span style="font-size: 11pt; font-family: Helvetica;">-<sup>15</sup>N, fractional</span> 5%-<sup>13</sup>C-labeled<span> (NC5) sample </span><span style="font-size: 11pt; font-family: Helvetica;">&nbsp;</span>


<span style="font-size: 11pt; font-family: Helvetica;">and give the Neri reference.</span>
<br> For RDC measurement:
*A secondary NC5 sample for RDC measurement.<br>
(Alternatively, for the H-N RDC measurement, a [U-<sup>15</sup>N]-labeled sample can be used. Rutger's uses a second NC5 sample, since this is all ready being prepared for the stereospecific assignments, and a <sup>13</sup>C CHSQC can be compared with the [U-<sup>15</sup>N, <sup>13</sup>C]-labeled sample, to check if the samples are the same.)
<br> For each protein that is a dimer in solution an extra sample may be required in addition to the samples above:
*1:1 mixture of natural abundance and [U-<sup>15</sup>N, <sup>13</sup>C]-labeled samples, for intermolecular NOE interpretation.
<br>


== Typical Rutgers University NMR Buffers<br>  ==
== Typical Rutgers University NMR Buffers<br>  ==


The protein production facility at Rutgers University uses three typical NMR buffers for the initial protein screening.  
The protein production facility at Rutgers University uses these three NMR buffers for the initial protein screening:&nbsp;<ref>Snyder, D, et.al. (2005). “Comparisons of NMR spectral quality and success in crystallization demonstrate that NMR and X-ray crystallography are complementary methods for small protein structure determination.” ''JACS'', '''127:''' 16505-16511. [http://www.ncbi.nlm.nih.gov/pubmed/16305237 pmid = 16305237] </ref>:<br>
 
<br>
 
*pH 4.5 NMR buffer:<span style="">&nbsp; </span>20 mM NH<sub>4</sub>OAc, 100 mM NaCl, 10 mM DTT, 5 mM CaCl<sub>2</sub>, 0.02% NaN<sub>3</sub>, 5% D<sub>2</sub>O
*pH 5.5 NMR buffer:<span style="">&nbsp; </span>20 mM NH<sub>4</sub>OAc, 100 mM NaCl, 10 mM DTT, 5 mM CaCl<sub>2</sub>, 0.02% NaN<sub>3</sub>, 5% D<sub>2</sub>O
*pH 6.5 NMR buffer:<span style="">&nbsp; </span>20 mM MES, 100 mM NaCl, 5 mM CaCl<sub>2</sub>, 10 mM DTT, 0.02% NaN<sub>3</sub>, 5% D<sub>2</sub>O


<br>  
<br>  


They are <ref>Snyder, D, et.al. (2005). “Comparisons of NMR spectral quality and success in crystallization demonstrate that NMR and X-ray crystallography are complementary methods for small protein structure determination.''JACS'', '''127:''' 16505-16511.pmid = </ref>:  
== Typical Rutgers University Protein Purification Protocol<br>  ==
 
''E.coli'' BL21(DE3) are fermented in MJ9 medium <ref>Jansson M, Li YC, Jendeberg L, Anderson S, Montelione GT, Nilsson B (1996) High-level production of uniformly 15N- and 13C-enriched fusion proteins in ''Escherichia coli''. J Biomol NMR 7: 131-141</ref>. Cell pellets are stored at -20º C.
 
#add <!--StartFragment--><font face="Verdana, Helvetica, Arial"><span style="font-size: 12px;">30 ml Lysis buffer</span></font> <!--EndFragment--> to the a frozen cell pellet and thaw.
#sonicate in ice bath
#centrifuge to remove insoluble part
#supernatant is added to an AkTAxpress<sup>TM</sup> system with a His TrapHP column followed by HiLoad16/60 Superdex 75 gel filtration chromatography.
#exchange buffer to screening buffer by concentrating, diluting with new buffer, reconcentrating to 0.3 - 1.0 mM with Amicon ultrafiltration concentrator (Millipore). <br><br>
 
== Typical University of Toronto (Arrowsmith proteomics NMR lab) NMR Buffers  ==
 
'''15N-labelled Glucose-based autoinduction media'''&nbsp;: <br>
 
This is for 15N-labelling of samples for screening so we can take advantage of autoinduction during the screening phase when we are dealing with a large number of samples, but be able to switch to IPTG-induction with <sup>13</sup>C-glucose as the sole carbon source for the smaller number of targets prepared for full NMR data collection, with high reproducibility. <br>
 
For each sample, dissolve 6.8 g of Na<sub>2</sub>HPO<sub>4</sub>, 3g of KH<sub>2</sub>PO<sub>4</sub>, 0.5g of NaCl, and 0.6g of <sup>15</sup>N-labelled NH<sub>4</sub>Cl, in 500 mL water and autoclave the media.&nbsp; <br>  
 
In a separate small beaker or falcon tube, mix the following: 1 mL of 1M MgSO<sub>4</sub> stock solution; 5 mg of biotin; 5 mg of thiamine<sup>.</sup>HCl; 10 uL of 1M ZnSO<sub>4</sub> stock solution; 100 uL of 1M CaCl<sub>2</sub> stock solution; 2.5 g of glucose; 1 g of lactose and enough water to make 10 mL. Stir or shake until the sugar dissolves. Filter sterilize through a 0.2 micron syringe filter into the autoclaved <sup>15</sup>N-labelled solution.
 
 
 
Follow the same fermentation protocol as with IPTG-induction method, except there is no need to monitor the cell density and omit the addition of IPTG. Grow the cells at 37C throughout.&nbsp;


<ref>{{cite journal | author = R.D. Johnson, L. Johnson, Y. Itoh, M. Kodama, H. Otani, and K. Kohmoto | year = 2000 | journal = Molecular Plant-Microbe Interactions | volume = 13 | pages = 742-753 | url = http://apsjournals.apsnet.org/doi/abs/10.1094/MPMI.2000.13.7.742?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub%3dncbi.nlm.nih.gov | pmid = 10875335 | doi = 10.1094/MPMI.2000.13.7.742 | title = Cloning and Characterization of a Cyclic Peptide Synthetase Gene from Alternaria alternata Apple Pathotype Whose Product Is Involved in AM-Toxin Synthesis and Pathogenicity}}</ref>  
<br>  


*pH 4.5 NMR buffer:<span style="">&nbsp; </span>20mM NH<sub>4</sub>OAc, 100mM NaCl, 10mM DTT, 5mM CaCl2, 0.02% NaN<sub>3</sub>, 5% D<sub>2</sub>O
Standard screening NMR buffers are:  
*pH 5.5 NMR buffer:<span style="">&nbsp; </span>20mM NH<sub>4</sub>OAc, 100mM NaCl, 10mM DTT, 5mM CaCl2, 0.02% NaN<sub>3</sub>, 5% D<sub>2</sub>O
*pH 6.5 NMR buffer:<span style="">&nbsp; </span>20mM MES, 100mM NaCl, 5 mM CaCl<sub>2</sub>, 10mM DTT, 0.02% NaN<sub>3</sub>, 5% D<sub>2</sub>O


<br>
<br>  


== Typical Rutgers University Protein Purification Protocol<br> ==
{| cellspacing="1" cellpadding="1" border="1" style="width: 548px; height: 238px;"
|-
| name
| buffer
| NaCl concentration
| pH
|-
| A5.0n300zd
| Sodium acetate
| 300 mM
| 5.0
|-
| Bt6.0n450zd
| bis-tris
| 450 mM
| 6.0
|-
| Bt6.5n450zd
| bis-tris
| 450 mM
| 6.5
|-
| M6.5n450zd
| MOPS
| 450 mM
| 6.5
|-
| P6.5n450zd
| Sodium phosphate
| 450 mM
| 6.5
|-
| P7.0n450zd
| Sodium phosphate
| 450 mM
| 7.0
|-
| H7.0n450zd
| HEPES
| 450 mM
| 7.0
|-
| H7.5n450zd
| HEPES
| 450 mM
| 7.5
|-
| T7.7n500zd
| tris
| 500 mM
| 7.7
|}


Coming soon...
<br>


== Typical University of Toronto NMR Buffers<br> ==
All standard screening buffers contain 10 mM of the buffer, 10 uM ZnSO4, 10 mM DTT, 0.01% NaN3, 1 mM benzamidine, 1x inhibitor cocktail, 5% D2O.<br>  


Some typical NMR buffers are: <br><br>  
<br>  


'''t7.3n250zd buffer:''' 10 mM tris, 250 mM NaCl, ~10 uM Zn++, 10 mM DTT, 0.01% NaN3,1 mM benzamidine, 1x inhibitor cocktail, 5% D2O, pH 7.3 <br>
The final NMR buffer for [U-<sup>15</sup>N, <sup>13</sup>C]-labeled sample depends on the protein of interest. all NMR buffers always contain&nbsp;: 0.01&nbsp;%&nbsp;NaN<sub>3</sub>, 1 mM&nbsp;benzamidine, 1x inhibitor cocktail.  


<br> '''m6.5n450zd buffer:''' 10 mM MOPS, 450 mM NaCl , ~10 uM Zn++, 10 mM DTT, 0.01% NaN3,1 mM benzamidine, 1x inhibitor cocktail, 5% D2O, pH 6.5
(1) If the protein has no cysteine in the sequence, do not bother to add ZnSO<sub>4</sub> and DTT (Zn ion will just be a nuisance and deuterated DTT is expensive).


== Typical University of Toronto Protein Purification Protocol<br>  ==
== Typical University of Toronto Protein Purification Protocol<br>  ==


Required buffers:
i015t8.5n500z&nbsp;: 15 mM imidazole, 10 mM tris, pH 8.5, 500 mM&nbsp;NaCl, 10 uM&nbsp;ZnSO<sub>4</sub>
i030t8.5n500z&nbsp;: 30 mM imidazole, 10 mM tris, pH 8.5, 500 mM&nbsp;NaCl, 10 uM&nbsp;ZnSO<sub>4</sub>
i500t8.5n500z&nbsp;: 500 mM imidazole, 10 mM tris, pH 8.5, 500 mM&nbsp;NaCl, 10 uM&nbsp;ZnSO<sub>4</sub>
1M DTT
1M&nbsp;benzamidine <br> <br>
#add 25 mL of i015t8.5n500z into a frozen cell pellet and thaw.<br>
#sonicate in ice bath
#spin down cell pellet
#transfer supernatant into new falcon tube and add 3 mL of nickel beads
#rock the tube for at least 30 minutes in cold.
#spin down the beads and discard the supernatant
#wash the beads with i015t8.5n500z twice and with i030t8.5n500z twice<br>
#in the final i030t8.5n500z wash,&nbsp; pour the beads unto gravity filter column <br>
#elute the protein with i500t8.5n500z
#add benzamidine, and add DTT<br>
#exchange buffer by concentrating, diluting with new buffer, reconcentrating in a vivaspin concentrator.<br> <br>
if it is [U-<sup>15</sup>N, <sup>13</sup>C]-labeled, add step


(9a) put sample in dialysis bag with protease to cut his-tag and dialyse&nbsp; against&nbsp; cleavage buffer


coming soon...
(9b) pass the sample through nickel beads again, then follow step (10) above. <br>


== References  ==
== References  ==


<references />
<references />
 
<br> {{reflist}} <br>

Latest revision as of 17:02, 8 March 2013

Samples for NMR

For each protein, we usually make the following samples:

  • [U-15N, 13C]-labeled sample, for resonance assignment and NOE interpretation.

A U-15N and fractional 5%-13C-labeled sample for stereospecific assignment of Val and Leu isopropyl methyl groups based on the method of Neri et. al. (Biochemistry 28, 7510-7516,1989) that we call the NC5 sample.  This sample is prepared using 5% U[1H,13C]-D-glucose and 95% unlabelled glucose in the E. coli minimal  growth media.


The NC5 sample is not [U-5%-13C,U-15N] since it is not uniformly 5% -13C labeled.  The incorporation of 13C in the methyl groups is biosynthetically directed by the E. coli

This sample can be called:

           U-15N, 5% biosynthetically directed 13C (NC5) sample or

            U-15N, fractional 5%-13C-labeled (NC5) sample  

and give the Neri reference.


For RDC measurement:

  • A secondary NC5 sample for RDC measurement.

(Alternatively, for the H-N RDC measurement, a [U-15N]-labeled sample can be used. Rutger's uses a second NC5 sample, since this is all ready being prepared for the stereospecific assignments, and a 13C CHSQC can be compared with the [U-15N, 13C]-labeled sample, to check if the samples are the same.)


For each protein that is a dimer in solution an extra sample may be required in addition to the samples above:

  • 1:1 mixture of natural abundance and [U-15N, 13C]-labeled samples, for intermolecular NOE interpretation.


Typical Rutgers University NMR Buffers

The protein production facility at Rutgers University uses these three NMR buffers for the initial protein screening: [1]:


  • pH 4.5 NMR buffer:  20 mM NH4OAc, 100 mM NaCl, 10 mM DTT, 5 mM CaCl2, 0.02% NaN3, 5% D2O
  • pH 5.5 NMR buffer:  20 mM NH4OAc, 100 mM NaCl, 10 mM DTT, 5 mM CaCl2, 0.02% NaN3, 5% D2O
  • pH 6.5 NMR buffer:  20 mM MES, 100 mM NaCl, 5 mM CaCl2, 10 mM DTT, 0.02% NaN3, 5% D2O


Typical Rutgers University Protein Purification Protocol

E.coli BL21(DE3) are fermented in MJ9 medium [2]. Cell pellets are stored at -20º C.

  1. add 30 ml Lysis buffer to the a frozen cell pellet and thaw.
  2. sonicate in ice bath
  3. centrifuge to remove insoluble part
  4. supernatant is added to an AkTAxpressTM system with a His TrapHP column followed by HiLoad16/60 Superdex 75 gel filtration chromatography.
  5. exchange buffer to screening buffer by concentrating, diluting with new buffer, reconcentrating to 0.3 - 1.0 mM with Amicon ultrafiltration concentrator (Millipore).

Typical University of Toronto (Arrowsmith proteomics NMR lab) NMR Buffers

15N-labelled Glucose-based autoinduction media :

This is for 15N-labelling of samples for screening so we can take advantage of autoinduction during the screening phase when we are dealing with a large number of samples, but be able to switch to IPTG-induction with 13C-glucose as the sole carbon source for the smaller number of targets prepared for full NMR data collection, with high reproducibility.

For each sample, dissolve 6.8 g of Na2HPO4, 3g of KH2PO4, 0.5g of NaCl, and 0.6g of 15N-labelled NH4Cl, in 500 mL water and autoclave the media. 

In a separate small beaker or falcon tube, mix the following: 1 mL of 1M MgSO4 stock solution; 5 mg of biotin; 5 mg of thiamine.HCl; 10 uL of 1M ZnSO4 stock solution; 100 uL of 1M CaCl2 stock solution; 2.5 g of glucose; 1 g of lactose and enough water to make 10 mL. Stir or shake until the sugar dissolves. Filter sterilize through a 0.2 micron syringe filter into the autoclaved 15N-labelled solution.


Follow the same fermentation protocol as with IPTG-induction method, except there is no need to monitor the cell density and omit the addition of IPTG. Grow the cells at 37C throughout. 


Standard screening NMR buffers are:


name buffer NaCl concentration pH
A5.0n300zd Sodium acetate 300 mM 5.0
Bt6.0n450zd bis-tris 450 mM 6.0
Bt6.5n450zd bis-tris 450 mM 6.5
M6.5n450zd MOPS 450 mM 6.5
P6.5n450zd Sodium phosphate 450 mM 6.5
P7.0n450zd Sodium phosphate 450 mM 7.0
H7.0n450zd HEPES 450 mM 7.0
H7.5n450zd HEPES 450 mM 7.5
T7.7n500zd tris 500 mM 7.7


All standard screening buffers contain 10 mM of the buffer, 10 uM ZnSO4, 10 mM DTT, 0.01% NaN3, 1 mM benzamidine, 1x inhibitor cocktail, 5% D2O.


The final NMR buffer for [U-15N, 13C]-labeled sample depends on the protein of interest. all NMR buffers always contain : 0.01 % NaN3, 1 mM benzamidine, 1x inhibitor cocktail.

(1) If the protein has no cysteine in the sequence, do not bother to add ZnSO4 and DTT (Zn ion will just be a nuisance and deuterated DTT is expensive).

Typical University of Toronto Protein Purification Protocol

Required buffers:

i015t8.5n500z : 15 mM imidazole, 10 mM tris, pH 8.5, 500 mM NaCl, 10 uM ZnSO4

i030t8.5n500z : 30 mM imidazole, 10 mM tris, pH 8.5, 500 mM NaCl, 10 uM ZnSO4

i500t8.5n500z : 500 mM imidazole, 10 mM tris, pH 8.5, 500 mM NaCl, 10 uM ZnSO4

1M DTT

1M benzamidine

  1. add 25 mL of i015t8.5n500z into a frozen cell pellet and thaw.
  2. sonicate in ice bath
  3. spin down cell pellet
  4. transfer supernatant into new falcon tube and add 3 mL of nickel beads
  5. rock the tube for at least 30 minutes in cold.
  6. spin down the beads and discard the supernatant
  7. wash the beads with i015t8.5n500z twice and with i030t8.5n500z twice
  8. in the final i030t8.5n500z wash,  pour the beads unto gravity filter column
  9. elute the protein with i500t8.5n500z
  10. add benzamidine, and add DTT
  11. exchange buffer by concentrating, diluting with new buffer, reconcentrating in a vivaspin concentrator.

if it is [U-15N, 13C]-labeled, add step

(9a) put sample in dialysis bag with protease to cut his-tag and dialyse  against  cleavage buffer

(9b) pass the sample through nickel beads again, then follow step (10) above.

References

  1. Snyder, D, et.al. (2005). “Comparisons of NMR spectral quality and success in crystallization demonstrate that NMR and X-ray crystallography are complementary methods for small protein structure determination.” JACS, 127: 16505-16511. pmid = 16305237
  2. Jansson M, Li YC, Jendeberg L, Anderson S, Montelione GT, Nilsson B (1996) High-level production of uniformly 15N- and 13C-enriched fusion proteins in Escherichia coli. J Biomol NMR 7: 131-141