Resonance Assignment/XEASY: Difference between revisions

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#[[XEASY Introduction|Introduction]]
#[[XEASY Introduction|Introduction]]  
#[[XEASY Spin system identification|Spin system identification]]  
#[[XEASY Spin system identification|Spin system identification]]  
#Backbone resonance assignment'''<br>'''  
#Backbone resonance assignment'''<br>'''  
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##Conventional spectra  
##Conventional spectra  
###[[HNCACB/CBCA(CO)NH]]  
###[[HNCACB/CBCA(CO)NH]]  
###HNCA/HN(CO)CA
###HNCO/HN(CA)CO
###NOESY/TOCSY
#Side chain resonance assignment  
#Side chain resonance assignment  
##Aliphatic  
##Aliphatic  
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####HAHB(CO)NH  
####HAHB(CO)NH  
####HCCH-COSY  
####HCCH-COSY  
####HCCH-TOCSY
####[[Side chain assignment with CN-NOESY in XEASY|Simultaneous NOESY]]  
####[[Side chain assignment with CN-NOESY in XEASY|Simultaneous NOESY]]  
####(H)CC-TOCSY-(CO)NH
####H(CC-TOCSY-CO)NH
##[[Aromatic side chain assignment with Aro-HCCH-COSY in XEASY|Aromatic]]  
##[[Aromatic side chain assignment with Aro-HCCH-COSY in XEASY|Aromatic]]  
###GFT-based spectra
##Additional side chain atoms
###Conventional spectra
###[[Trp e1 and d1 assignment with NOESY|Trp e1 NH and d1 CH]]
##Other
###Trp e1 NH and d1 CH  
###[[Met methyl assignment with NOESY|Met e CH3 ]]  
###[[Met methyl assignment with NOESY|Met e CH3 ]]  
###[[Amide Side Chain assignment with NOESY|Asn d2 and Gln e2 NH2]]  
###[[Amide Side Chain assignment with NOESY|Asn d2 and Gln e2 NH2]]
#NOESY peak integration

Latest revision as of 16:21, 5 January 2010

  1. Introduction
  2. Spin system identification
  3. Backbone resonance assignment
    1. GFT-based spectra
      1. HNCACAB/CABCA(CO)NH
    2. Conventional spectra
      1. HNCACB/CBCA(CO)NH
  4. Side chain resonance assignment
    1. Aliphatic
      1. GFT-based spectra
        1. (4,3)D GFT HABCAB(CO)NHN
        2. (4,3)D GFT HCCH
      2. Conventional spectra
        1. HAHB(CO)NH
        2. HCCH-COSY
        3. Simultaneous NOESY
    2. Aromatic
    3. Additional side chain atoms
      1. Trp e1 NH and d1 CH
      2. Met e CH3
      3. Asn d2 and Gln e2 NH2