Resonance Assignment/XEASY: Difference between revisions

From NESG Wiki
Jump to navigation Jump to search
No edit summary
No edit summary
 
(One intermediate revision by one other user not shown)
Line 13: Line 13:
###Conventional spectra  
###Conventional spectra  
####HAHB(CO)NH  
####HAHB(CO)NH  
####HCCH-COSY
####HCCH-COSY  
####[[Side chain assignment with CN-NOESY in XEASY|Simultaneous NOESY]]  
####[[Side chain assignment with CN-NOESY in XEASY|Simultaneous NOESY]]  
##[[Aromatic side chain assignment with Aro-HCCH-COSY in XEASY|Aromatic]]  
##[[Aromatic side chain assignment with Aro-HCCH-COSY in XEASY|Aromatic]]  
##Additional side chain atoms  
##Additional side chain atoms  
###Trp e1 NH and d1 CH  
###[[Trp e1 and d1 assignment with NOESY|Trp e1 NH and d1 CH]]
###[[Met methyl assignment with NOESY|Met e CH3 ]]  
###[[Met methyl assignment with NOESY|Met e CH3 ]]  
###[[Amide Side Chain assignment with NOESY|Asn d2 and Gln e2 NH2]]  
###[[Amide Side Chain assignment with NOESY|Asn d2 and Gln e2 NH2]]
#NOESY peak integration

Latest revision as of 16:21, 5 January 2010

  1. Introduction
  2. Spin system identification
  3. Backbone resonance assignment
    1. GFT-based spectra
      1. HNCACAB/CABCA(CO)NH
    2. Conventional spectra
      1. HNCACB/CBCA(CO)NH
  4. Side chain resonance assignment
    1. Aliphatic
      1. GFT-based spectra
        1. (4,3)D GFT HABCAB(CO)NHN
        2. (4,3)D GFT HCCH
      2. Conventional spectra
        1. HAHB(CO)NH
        2. HCCH-COSY
        3. Simultaneous NOESY
    2. Aromatic
    3. Additional side chain atoms
      1. Trp e1 NH and d1 CH
      2. Met e CH3
      3. Asn d2 and Gln e2 NH2
Retrieved from "https://nesgwiki.chem.buffalo.edu/index.php?title=Resonance_Assignment/XEASY&oldid=3435"