Common NMR experiment sets: Difference between revisions

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The following experiments are recorded for NC samples  
The following experiments are recorded for NC samples  


Common experiments  
{|  cellspacing="0" cellpadding="0"
 
|-
! Common experiments
! GFT protocol
! Conventional protocol
! Fractionally 13C-labeled samples
|- valign="top"
|
*1D  
*1D  
*1D 15N T1  
*1D 15N T1  
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*3D 15N/13Cali/13Caro-resolved [1H,1H]-NOESY
*3D 15N/13Cali/13Caro-resolved [1H,1H]-NOESY


<br>
|
 
GFT protocol
 
*(3,2)D GFT HN<u>NCO</u> or 3D HNCO<br>  
*(3,2)D GFT HN<u>NCO</u> or 3D HNCO<br>  
*(4,3)D GFT&nbsp;<u>CABCA</u>(CO)NH<br>  
*(4,3)D GFT&nbsp;<u>CABCA</u>(CO)NH<br>  
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*(4,3)D <u>GFT&nbsp;HC</u>CH-COSY aromatic
*(4,3)D <u>GFT&nbsp;HC</u>CH-COSY aromatic


<br>
|
 
Conventional protocol
 
*3D&nbsp;HNCO  
*3D&nbsp;HNCO  
*3D&nbsp;HN(CA)CO  
*3D&nbsp;HN(CA)CO  
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*(H)CCH-COSY aromatic
*(H)CCH-COSY aromatic


<br>
|
 
Fractionally 13C-labeled samples
 
*2D [13C,1H]-CT-HSQC, 28ms delay - optimized for methyl groups<br>  
*2D [13C,1H]-CT-HSQC, 28ms delay - optimized for methyl groups<br>  
*2D [13C,1H]-CT-HSQC, 56ms delay (optional)<br>  
*2D [13C,1H]-CT-HSQC, 56ms delay (optional)<br>  
*2D [13C,1H]-CT-HSQC, 42ms delay (optional)  
*2D [13C,1H]-CT-HSQC, 42ms delay (optional)  
*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional - can also be run for the NC sample)<br>
*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional - can also be run for the NC sample)<br>
 
|}
<br>


== Arrowsmith group  ==
== Arrowsmith group  ==
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Data acquisition is taylored for [[Abacus|ABACUS]] approach for resonance assignment. Most 3D spectra are collected with [[Setting up non-uniformly sampled spectra|Non-uniform sampling]] and processed with [[Processing non-uniformly sampled spectra with Multidimensional Decomposition|Multidimensional decomposition]],  
Data acquisition is taylored for [[Abacus|ABACUS]] approach for resonance assignment. Most 3D spectra are collected with [[Setting up non-uniformly sampled spectra|Non-uniform sampling]] and processed with [[Processing non-uniformly sampled spectra with Multidimensional Decomposition|Multidimensional decomposition]],  


*1D presaturation
<br>
*1D 15N T1
*1D 15N T2
*2D [15N,1H]-HSQC
*2D [13C,1H]-HSQC aliphatic
*2D [13C,1H]-HSQC aromatic
*2D [13C,1H]-CT-HSQC aliphatic
*2D [13C,1H]-CT-HSQC aromatic (optional)
*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
*2D HE(CECDCG)CB (optional)


{|
{| cellspacing="0" cellpadding="0"
|-  
|-
! NH-rooted&nbsp;  
! NH-rooted&nbsp;  
! CH-rooted&nbsp;
! CH-rooted (aliphatic)
! Aromatic
! CH-rooted (aromatic)
! Other
|- valign="top"
|- valign="top"
|  
|  
*2D [15N,1H]-HSQC
*3D 15N-NOESY-HSQC  
*3D 15N-NOESY-HSQC  
*3D HNCO  
*3D HNCO  
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|  
|  
*2D [13C,1H]-HSQC
*2D [13C,1H]-CT-HSQC
*3D 13C-NOESY-HSQC
*3D (H)CCH-TOCSY  
*3D (H)CCH-TOCSY  
*3D H(C)CH-TOCSY  
*3D H(C)CH-TOCSY
*3D 13C-NOESY-HSQC


|  
|  
*2D [13C,1H]-HSQC
*2D [13C,1H]-CT-HSQC (optional)
*3D 13C-NOESY-HSQC
*3D (H)CCH-TOCSY (optional)  
*3D (H)CCH-TOCSY (optional)  
*3D H(C)CH-TOCSY (optional)  
*3D H(C)CH-TOCSY (optional)
*3D 13C-NOESY-HSQC
 
|
*1D presaturation
*1D 15N T1
*1D 15N T2
*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
*2D HE(CECDCG)CB (optional)
*2D HD(CDCG)CB (optional)


|}
|}

Latest revision as of 11:07, 8 January 2010

Szyperski group

The following experiments are recorded for NC samples

Common experiments GFT protocol Conventional protocol Fractionally 13C-labeled samples
  • 1D
  • 1D 15N T1
  • 1D 15N T2
  • 2D [15N,1H]-HSQC
  • 2D [15N,1H]-HSQC with wide spectral width in 15N (optional)
  • 2D [13C,1H]-HSQC aliphatic
  • 2D [13C,1H]-HSQC aromatic (optional)
  • 2D [13C,1H]-CT-HSQC aliphatic
  • 2D [13C,1H]-CT-HSQC aromatic
  • 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
  • 3D HN(CA)CO (optional)
  • 3D (H)CCH-TOCSY
  • 2D HE(CECDCG)CB (optional)
  • 3D 15N/13Cali/13Caro-resolved [1H,1H]-NOESY
  • (3,2)D GFT HNNCO or 3D HNCO
  • (4,3)D GFT CABCA(CO)NH
  • (4,3)D GFT HNCABCA
  • (4,3)D GFT HABCAB(CO)NH
  • (4,3)D GFT HCCH aliphatic
  • (4,3)D GFT HCCH-COSY aromatic
  • 3D HNCO
  • 3D HN(CA)CO
  • 3D CBCA(CO)NH
  • 3D HNCACB
  • 3D HBHA(CO)NH
  • (H)CCH-COSY aliphatic
  • (H)CCH-COSY aromatic
  • 2D [13C,1H]-CT-HSQC, 28ms delay - optimized for methyl groups
  • 2D [13C,1H]-CT-HSQC, 56ms delay (optional)
  • 2D [13C,1H]-CT-HSQC, 42ms delay (optional)
  • 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional - can also be run for the NC sample)

Arrowsmith group

Data acquisition is taylored for ABACUS approach for resonance assignment. Most 3D spectra are collected with Non-uniform sampling and processed with Multidimensional decomposition,


NH-rooted  CH-rooted (aliphatic) CH-rooted (aromatic) Other
  • 2D [15N,1H]-HSQC
  • 3D 15N-NOESY-HSQC
  • 3D HNCO
  • 3D HNCA
  • 3D CBCA(CO)NH
  • 3D HBHA(CO)NH
  • 3D C(CCO)NH-TOCSY (optional)
  • 3D H(CCCO)NH-TOCSY (optional)
  • 2D [13C,1H]-HSQC
  • 2D [13C,1H]-CT-HSQC
  • 3D 13C-NOESY-HSQC
  • 3D (H)CCH-TOCSY
  • 3D H(C)CH-TOCSY
  • 2D [13C,1H]-HSQC
  • 2D [13C,1H]-CT-HSQC (optional)
  • 3D 13C-NOESY-HSQC
  • 3D (H)CCH-TOCSY (optional)
  • 3D H(C)CH-TOCSY (optional)
  • 1D presaturation
  • 1D 15N T1
  • 1D 15N T2
  • 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
  • 2D HE(CECDCG)CB (optional)
  • 2D HD(CDCG)CB (optional)
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