Protein purification: Difference between revisions

Revision as of 16:33, 11 November 2009

For each protein, we usually make the following samples:

100% 15N, 100% 13C uniform labeled sample, for resonance assignment and NOE interpretion.
100% 15N, 5% 13C labeled sample, for stereospecific assignment of VAL and LEU isopropyl moieties.

For RDC measurement:

For each protein that exist as dimer in solution an extra sample may be required in addition to the samples above:

1:1 unlabeled and 100% 15N, 100% 13C uniformed labeled mixed sample, for intermolecular NOE interpretation

@@ Line 1: / Line 1: @@
-Samples for NMR
+== Samples for NMR <br>  ==
-<br>
-#  For each protein, we usually order the following samples:
-    * 100% 15N, 100% 13C uniform labeled sample, for resonance assignment and NOE interpretion.
+For each protein, we usually make the following samples:
-    * 100% 15N, 5% 13C labeled sample, for stereo specific assignment of VAL and LEU isopropyl moieties.
-#  For RDC measurements
+*&nbsp;&nbsp; 100% 15N, 100% 13C uniform labeled sample, for resonance assignment and NOE interpretion.
-    * A secondary 100% 15N, 5% 13C labeled sample, for RDC measurement.
+*&nbsp;&nbsp; 100% 15N, 5% 13C labeled sample, for stereospecific assignment of VAL and LEU isopropyl moieties.
-# For each protein that exist as dimer in solution extra sample may be required in addition to the two samples above
+For RDC measurement:
-    * 1:1 unlabeled and 100% 15N, 100% 13C uniformed labeled mixed sample, for intermolecular NOE interpretation
+*&nbsp;&nbsp; A secondary 100% 15N, 5% 13C labeled sample, for RDC measurement.
+For each protein that exist as dimer in solution an extra sample may be required in addition to the samples above:
+*&nbsp;&nbsp; 1:1 unlabeled and 100% 15N, 100% 13C uniformed labeled mixed sample, for intermolecular NOE interpretation
+== Typical Rutgers University Protocol ==
+== Typical University of Toronto Protocol ==