Metabonomics SOP: Difference between revisions

From NESG Wiki
Jump to navigation Jump to search
No edit summary
No edit summary
Line 5: Line 5:
== Safety Protocols  ==
== Safety Protocols  ==


=== Contact information  ===


=== Contact information ===


=== NMR Safety Rules ===
*'''Dr. Khalid Ahsan'''Chemistry Department Safety Officer


==== Cryogenic liquids ====
ahsan@buffalo.edu


==== Magnetic materials ====
716-645-4115


==== Physical contact ====
*'''Dr. Leonard Borzynski'''


==== Injury reporting ====
Manager, Biosafety Division, Environment, Health & Safety (EH&S)


=== Biosafety ===
lenb@buffalo.edu


==== Safety training ====
716-829-3301; 716-829-2401 (sharps injury reporting)


==== Training records ====
*'''University Police'''


==== Vaccination ====
716-645-2222


==== Universal safety precautions and lab safety policies ====
Mr. Erwin Garcia


==== Injury logs and reporting ====
PhD student


=== Additional documents ===
egarcia3@buffalo.edu


==== Chemical hygiene plan ====
716-908-8567


==== Exposure control plan ====
*'''Dr. Dinesh Sukumaran'''
 
Director, NMR Center<br>
 
dks@buffalo.edu
 
716-725-1107
 
*'''Dr. Thomas Szyperski'''
 
Principal Investigator
 
szypersk@buffalo.edu
 
716-472-7075
 
*'''Szyperski Lab'''
 
716-645-4302, 716-645-4303, 716-645-4304, 716-645-4312
 
*'''University at Buffalo Health Services'''
 
716-829-3316
 
*'''Millard Fillmore Suburban Hospital'''
 
1540 Maple Road
 
Williamsville, NY 14221
 
716- 568-3600
 
*'''STERICYCLE'''
 
http://www.stericycle.com/medical-waste-disposal/customer-pickup-request.html
 
1-866-783-7422
 
 
=== NMR Safety Rules  ===
 
==== Cryogenic liquids  ====
 
 
#No person is allowed to use cryogenic liquids without training and permissio
#Dr. Sukumaran should be notified first before any attempt to use cryogenic liquids
#Protective glasses and thermal gloves should be used when working with cryogenic&nbsp;liquids
#In the event of a magnet quench, all occupants must exit the NMR lab immediately
#Any accident of exposure to cryogenic liquids must be reported to Dr. Sukumaran&nbsp;and Dr. Khalid Ahsan.
 
 
==== Magnetic materials  ====
 
 
#Persons with metallic implants (e.g. cardiac pacemakers, orthopedic pins, plates,&nbsp;arterial clips, etc.) must keep at least 10 feet distance from the magnets
#Metal and magnetic objects (e.g spatulas, carts, keys, ATM cards, wristwatches,&nbsp;laptops, magnetic tapes, disks, etc.) must be left in the wet lab room or on the table&nbsp;outside the NMR room
 
 
==== Physical contact  ====
 
 
#Don’t lean on the magnet
#Use the stairs provided for the spectrometers when loading the sample
 
 
==== Injury reporting  ====
 
#Report any injury to Dr. Sukumaran and Dr. Ashan
 
=== Biosafety  ===
 
==== Safety training  ====
 
==== Training records  ====
 
==== Vaccination  ====
 
==== Universal safety precautions and lab safety policies  ====
 
==== Injury logs and reporting  ====
 
=== Additional documents  ===
 
==== Chemical hygiene plan  ====
 
==== Exposure control plan ====


== Sample Storage and Preparation ==
== Sample Storage and Preparation ==

Revision as of 17:05, 25 January 2012

 

NMR

Safety Protocols

Contact information

  • Dr. Khalid AhsanChemistry Department Safety Officer

ahsan@buffalo.edu

716-645-4115

  • Dr. Leonard Borzynski

Manager, Biosafety Division, Environment, Health & Safety (EH&S)

lenb@buffalo.edu

716-829-3301; 716-829-2401 (sharps injury reporting)

  • University Police

716-645-2222

Mr. Erwin Garcia

PhD student

egarcia3@buffalo.edu

716-908-8567

  • Dr. Dinesh Sukumaran

Director, NMR Center

dks@buffalo.edu

716-725-1107

  • Dr. Thomas Szyperski

Principal Investigator

szypersk@buffalo.edu

716-472-7075

  • Szyperski Lab

716-645-4302, 716-645-4303, 716-645-4304, 716-645-4312

  • University at Buffalo Health Services

716-829-3316

  • Millard Fillmore Suburban Hospital

1540 Maple Road

Williamsville, NY 14221

716- 568-3600

  • STERICYCLE

http://www.stericycle.com/medical-waste-disposal/customer-pickup-request.html

1-866-783-7422


NMR Safety Rules

Cryogenic liquids

  1. No person is allowed to use cryogenic liquids without training and permissio
  2. Dr. Sukumaran should be notified first before any attempt to use cryogenic liquids
  3. Protective glasses and thermal gloves should be used when working with cryogenic liquids
  4. In the event of a magnet quench, all occupants must exit the NMR lab immediately
  5. Any accident of exposure to cryogenic liquids must be reported to Dr. Sukumaran and Dr. Khalid Ahsan.


Magnetic materials

  1. Persons with metallic implants (e.g. cardiac pacemakers, orthopedic pins, plates, arterial clips, etc.) must keep at least 10 feet distance from the magnets
  2. Metal and magnetic objects (e.g spatulas, carts, keys, ATM cards, wristwatches, laptops, magnetic tapes, disks, etc.) must be left in the wet lab room or on the table outside the NMR room


Physical contact

  1. Don’t lean on the magnet
  2. Use the stairs provided for the spectrometers when loading the sample


Injury reporting

  1. Report any injury to Dr. Sukumaran and Dr. Ashan

Biosafety

Safety training

Training records

Vaccination

Universal safety precautions and lab safety policies

Injury logs and reporting

Additional documents

Chemical hygiene plan

Exposure control plan

Sample Storage and Preparation

Serum

Urine

Others:Non-biofluids

Pipetting

Labeling of NMR specimens

Data Acquisition

Profiling of serum

Profiling of urine

Profiling of other specimens:Non-biofluids

For Resonance Assignment

Spiking of Metabolites

Data Archiving

Data Processing

Phase Correction

1D PC

2D PC

Baseline Correction

1D BC

2D BC

Peak Alignment: Recursive Segment-wise

Spectral Quality Assessment (FOM)

Operator Certification?

Targeted Profiling--FOM?

Resonance Assignment

Development of Metabolic Models?

Statistics

Retrieved from "https://nesgwiki.chem.buffalo.edu/index.php?title=Metabonomics_SOP&oldid=3927"