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== Safety Protocols == | == Safety Protocols == | ||
Everyone working in the NMR laboratory in ‘Statler Commissary’ is expected to act professionally and environmentally responsible. Our laboratory is committed to minimize the risk of personal and environmental hazards involved in working with high-field magnets and handling biological fluids. Thus, the protocols herein are established. These protocols are specifically tailored to our laboratory and subject to change if better procedures are identified in minimizing such risks. | |||
=== Contact information === | === Contact information === | ||
*'''Dr. Khalid Ahsan'''Chemistry Department Safety Officer | *'''Dr. Khalid Ahsan'''Chemistry Department Safety Officer | ||
ahsan@buffalo.edu | ahsan@buffalo.edu | ||
716-645-4115 | 716-645-4115 | ||
*'''Dr. Leonard Borzynski''' | *'''Dr. Leonard Borzynski''' | ||
Manager, Biosafety Division, Environment, Health & Safety (EH&S) | Manager, Biosafety Division, Environment, Health & Safety (EH&S) | ||
lenb@buffalo.edu | lenb@buffalo.edu | ||
716-829-3301; 716-829-2401 (sharps injury reporting) | 716-829-3301; 716-829-2401 (sharps injury reporting) | ||
*'''University Police''' | *'''University Police''' | ||
716-645-2222 | 716-645-2222 | ||
Mr. Erwin Garcia | Mr. Erwin Garcia | ||
PhD student | PhD student | ||
egarcia3@buffalo.edu | egarcia3@buffalo.edu | ||
716-908-8567 | 716-908-8567 | ||
*'''Dr. Dinesh Sukumaran''' | *'''Dr. Dinesh Sukumaran''' | ||
Director, NMR Center<br> | Director, NMR Center<br> | ||
dks@buffalo.edu | dks@buffalo.edu | ||
716-725-1107 | 716-725-1107 | ||
*'''Dr. Thomas Szyperski''' | *'''Dr. Thomas Szyperski''' | ||
Principal Investigator | Principal Investigator | ||
szypersk@buffalo.edu | szypersk@buffalo.edu | ||
716-472-7075 | 716-472-7075 | ||
*'''Szyperski Lab''' | *'''Szyperski Lab''' | ||
716-645-4302, 716-645-4303, 716-645-4304, 716-645-4312 | 716-645-4302, 716-645-4303, 716-645-4304, 716-645-4312 | ||
*'''University at Buffalo Health Services''' | *'''University at Buffalo Health Services''' | ||
716-829-3316 | 716-829-3316 | ||
*'''Millard Fillmore Suburban Hospital''' | *'''Millard Fillmore Suburban Hospital''' | ||
1540 Maple Road | 1540 Maple Road | ||
Williamsville, NY 14221 | Williamsville, NY 14221 | ||
716- 568-3600 | 716- 568-3600 | ||
*'''STERICYCLE''' | *'''STERICYCLE''' | ||
http://www.stericycle.com/medical-waste-disposal/customer-pickup-request.html | http://www.stericycle.com/medical-waste-disposal/customer-pickup-request.html | ||
1-866-783-7422 | 1-866-783-7422 | ||
<br> | |||
=== NMR Safety Rules === | === NMR Safety Rules === | ||
| Line 77: | Line 81: | ||
==== Cryogenic liquids ==== | ==== Cryogenic liquids ==== | ||
#No person is allowed to use cryogenic liquids without training and permissio | |||
#No person is allowed to use cryogenic liquids without training and permissio | #Dr. Sukumaran should be notified first before any attempt to use cryogenic liquids | ||
#Dr. Sukumaran should be notified first before any attempt to use cryogenic liquids | #Protective glasses and thermal gloves should be used when working with cryogenic liquids | ||
#Protective glasses and thermal gloves should be used when working with cryogenic liquids | #In the event of a magnet quench, all occupants must exit the NMR lab immediately | ||
#In the event of a magnet quench, all occupants must exit the NMR lab immediately | |||
#Any accident of exposure to cryogenic liquids must be reported to Dr. Sukumaran and Dr. Khalid Ahsan. | #Any accident of exposure to cryogenic liquids must be reported to Dr. Sukumaran and Dr. Khalid Ahsan. | ||
<br> | |||
==== Magnetic materials ==== | ==== Magnetic materials ==== | ||
#Persons with metallic implants (e.g. cardiac pacemakers, orthopedic pins, plates, arterial clips, etc.) must keep at least 10 feet distance from the magnets | |||
#Persons with metallic implants (e.g. cardiac pacemakers, orthopedic pins, plates, arterial clips, etc.) must keep at least 10 feet distance from the magnets | |||
#Metal and magnetic objects (e.g spatulas, carts, keys, ATM cards, wristwatches, laptops, magnetic tapes, disks, etc.) must be left in the wet lab room or on the table outside the NMR room | #Metal and magnetic objects (e.g spatulas, carts, keys, ATM cards, wristwatches, laptops, magnetic tapes, disks, etc.) must be left in the wet lab room or on the table outside the NMR room | ||
<br> | |||
==== Physical contact ==== | ==== Physical contact ==== | ||
#Don’t lean on the magnet | |||
#Don’t lean on the magnet | |||
#Use the stairs provided for the spectrometers when loading the sample | #Use the stairs provided for the spectrometers when loading the sample | ||
<br> | |||
==== Injury reporting ==== | ==== Injury reporting ==== | ||
| Line 105: | Line 109: | ||
=== Biosafety === | === Biosafety === | ||
==== Safety training ==== | ==== Safety training ==== | ||
===== Schedule for a discussion on the following should be made with Mr. Garcia (et. al.) ===== | ===== Schedule for a discussion on the following should be made with Mr. Garcia (et. al.) ===== | ||
#Exposure Control Plan | |||
#Exposure Control Plan | #Metabonomics lab set up in Statler | ||
#Metabonomics lab set up in Statler | #[http://www.osha.gov/pls/oshaweb/owadisp.show_document?p_table=STANDARDS&p_id=10051 Occupational Safety and Health Administration standards Regulations (Standards - 29 CFR) Bloodborne Pathogens] | ||
#[http://www.osha.gov/pls/oshaweb/owadisp.show_document?p_table=STANDARDS&p_id=10051 | |||
#Questions regarding other aspects of biosafety can be directed to Mr. Leonard Borzynski of EH&S. | #Questions regarding other aspects of biosafety can be directed to Mr. Leonard Borzynski of EH&S. | ||
<br> | |||
==== Training records ==== | ==== Training records ==== | ||
Write a paragraph on the WIKI regarding the date, location, resource person and bullet points of biosafety topics discussed | |||
<br> | |||
==== Vaccination ==== | ==== Vaccination ==== | ||
There are three shots that need to be legally administered to people who are front liners (have direct physical contact with blood and blood products): | |||
<br> | |||
#Undergrad/Graduate students: Call University at Buffalo Health Services to schedule an appointment. | #Undergrad/Graduate students: Call University at Buffalo Health Services to schedule an appointment. | ||
#Post-docs: Make sure that you have an active health insurance. Search online for health providers that are affiliated with your current health insurance and schedule an appointment for vaccination. If you have a family doctor, he is also allowed to administer the shots, if he agrees. | #Post-docs: Make sure that you have an active health insurance. Search online for health providers that are affiliated with your current health insurance and schedule an appointment for vaccination. If you have a family doctor, he is also allowed to administer the shots, if he agrees. | ||
<br> | |||
==== Universal safety precautions and lab safety policies ==== | ==== Universal safety precautions and lab safety policies ==== | ||
#Prepare 10% (v/v) bleach-water solution before handling serum samples. Keep the solution only for a week. Fill out the form that is posted beside the metabonomics hood for bleach solution preparation indicating the date and name of person who prepared it | |||
#Wash hands with hand soap and water before and after sample preparation | |||
#Prepare 10% (v/v) bleach-water solution before handling serum samples. Keep the solution only for a week. Fill out the form that is posted beside the metabonomics hood for bleach solution preparation indicating the date and name of person who prepared it | #Wear double purple nitrile gloves whenever you are working with blood and other potentially infectious materials | ||
#Wash hands with hand soap and water before and after sample preparation | #Wear full body lab coats at the time | ||
#Wear double purple nitrile gloves whenever you are working with blood and other potentially infectious materials | #Wear safety glasses | ||
#Wear full body lab coats at the time | #Dispose used pipet tips, Eppendorf tubes and gloves that came in contact with serum and serum products in their designated biohazardous waste container. Contact STERICYCLE to schedule for pick up if these containers get filled. Note that the schedules should be made at least 2 days prior to pick up dates. | ||
#Wear safety glasses | #Disinfect contaminated safety glasses, lab coats and clothing that were contaminated with serum and serum products by soaking them in a freshly prepared 10% (v/v) bleach-water solution | ||
#Dispose used pipet tips, Eppendorf tubes and gloves that came in contact with serum and serum products in their designated biohazardous waste container. Contact STERICYCLE to schedule for pick up if these containers get filled. Note that the schedules should be made at least 2 days prior to pick up dates. | #Eating and drinking is strictly prohibited inside the NMR lab | ||
#Disinfect contaminated safety glasses, lab coats and clothing that were contaminated with serum and serum products by soaking them in a freshly prepared 10% (v/v) bleach-water solution | #In the event of blood serum spill, cover the spill with freshly prepared bleach solution and let it stand for at least an hour before wiping it off with paper towels. Redisinfect the surface with freshly prepared bleach solution. Throw the used paper towels in the biohardous waste container. | ||
#Eating and drinking is strictly prohibited inside the NMR lab | #For NMR tube breakage, recover as much sample as possible from the tube, disinfect the broken tube by soaking it in freshly prepared bleach solution and disinfect the surface. Throw the disinfected glass fragments in the broken glass container | ||
#In the event of blood serum spill, cover the spill with freshly prepared bleach solution and let it stand for at least an hour before wiping it off with paper towels. Redisinfect the surface with freshly prepared bleach solution. Throw the used paper towels in the biohardous waste container. | #In the event that the serum sample was broken inside the probe, immediately inform Dr. Sukumaran. Isopropyl alcohol must be used as a cleaner and disinfectant for the probe. Don’ t use bleach. Probe cleaning should only be performed with Dr. Sukumaran. If the probe/spectrometer needs fixing, inform the person who will fix the problem the potentially hazardous nature of the sample before he starts to work | ||
#For NMR tube breakage, recover as much sample as possible from the tube, disinfect the broken tube by soaking it in freshly prepared bleach solution and disinfect the surface. Throw the disinfected glass fragments in the broken glass container | #During data collection, post notes announcing that data acquisition for a potentially hazardous sample is on going | ||
#In the event that the serum sample was broken inside the probe, immediately inform Dr. Sukumaran. Isopropyl alcohol must be used as a cleaner and disinfectant for the probe. Don’ t use bleach. Probe cleaning should only be performed with Dr. Sukumaran. If the probe/spectrometer needs fixing, inform the person who will fix the problem the potentially hazardous nature of the sample before he starts to work | #Wipe the NMR tube with Kimwipes before loading the sample to minimize transfer of dirt to the probe | ||
#During data collection, post notes announcing that data acquisition for a potentially hazardous sample is on going | #If you generate garbage (e.g. used paper towels, Kimwipes, parafilms, or any clutter in general), throw them in regular waste bin | ||
#Wipe the NMR tube with Kimwipes before loading the sample to minimize transfer of dirt to the probe | |||
#If you generate garbage (e.g. used paper towels, Kimwipes, parafilms, or any clutter in general), throw them in regular waste bin | |||
#Clean your working area before leaving the lab | #Clean your working area before leaving the lab | ||
<br> | |||
==== Injury logs and reporting ==== | ==== Injury logs and reporting ==== | ||
#In case of fire or magnet quench, call the University police, Dr. Sukumaran and Dr. Szyperski. | |||
#In case of fire or magnet quench, call the University police, Dr. Sukumaran and Dr. Szyperski. | #If you cut or puncture yourself while handling potentially biohazardous biofluids, wash the ezposed area with soap and water and seek medical attention from Milliard Filmore Suburban Hospital or from Health Services as soon as possible. | ||
#If you cut or puncture yourself while handling potentially biohazardous biofluids, wash the ezposed area with soap and water and seek medical attention from Milliard Filmore Suburban Hospital or from Health Services as soon as possible. | #Report the incident to Dr. Szyperski, Dr. Ashan and Dr. Sukumaran | ||
#Report the incident to Dr. Szyperski, Dr. Ashan and Dr. Sukumaran | #You should also contact EH&S (sharps injury reporting) | ||
#You should also contact EH&S (sharps injury reporting) | #Fill out an accident report form (http://hr.buffalo.edu/files/phatfile/Workers_Comp.pdf) and follow the procedures therein | ||
#Fill out an accident report form (http://hr.buffalo.edu/files/phatfile/Workers_Comp.pdf) and follow the procedures therein | |||
#Record the incident in ‘Sharp Injury Log book’ | #Record the incident in ‘Sharp Injury Log book’ | ||
=== Additional documents === | === Additional documents === | ||
==== Chemical hygiene plan ==== | ==== Chemical hygiene plan ==== | ||
==== Exposure control plan | ==== Exposure control plan<span style="font-size: 15px;" /> ==== | ||
== Sample Storage and Preparation == | == Sample Storage and Preparation == | ||
Revision as of 17:13, 25 January 2012
NMR
Safety Protocols
Everyone working in the NMR laboratory in ‘Statler Commissary’ is expected to act professionally and environmentally responsible. Our laboratory is committed to minimize the risk of personal and environmental hazards involved in working with high-field magnets and handling biological fluids. Thus, the protocols herein are established. These protocols are specifically tailored to our laboratory and subject to change if better procedures are identified in minimizing such risks.
Contact information
- Dr. Khalid AhsanChemistry Department Safety Officer
ahsan@buffalo.edu
716-645-4115
- Dr. Leonard Borzynski
Manager, Biosafety Division, Environment, Health & Safety (EH&S)
lenb@buffalo.edu
716-829-3301; 716-829-2401 (sharps injury reporting)
- University Police
716-645-2222
Mr. Erwin Garcia
PhD student
egarcia3@buffalo.edu
716-908-8567
- Dr. Dinesh Sukumaran
Director, NMR Center
dks@buffalo.edu
716-725-1107
- Dr. Thomas Szyperski
Principal Investigator
szypersk@buffalo.edu
716-472-7075
- Szyperski Lab
716-645-4302, 716-645-4303, 716-645-4304, 716-645-4312
- University at Buffalo Health Services
716-829-3316
- Millard Fillmore Suburban Hospital
1540 Maple Road
Williamsville, NY 14221
716- 568-3600
- STERICYCLE
http://www.stericycle.com/medical-waste-disposal/customer-pickup-request.html
1-866-783-7422
NMR Safety Rules
Cryogenic liquids
- No person is allowed to use cryogenic liquids without training and permissio
- Dr. Sukumaran should be notified first before any attempt to use cryogenic liquids
- Protective glasses and thermal gloves should be used when working with cryogenic liquids
- In the event of a magnet quench, all occupants must exit the NMR lab immediately
- Any accident of exposure to cryogenic liquids must be reported to Dr. Sukumaran and Dr. Khalid Ahsan.
Magnetic materials
- Persons with metallic implants (e.g. cardiac pacemakers, orthopedic pins, plates, arterial clips, etc.) must keep at least 10 feet distance from the magnets
- Metal and magnetic objects (e.g spatulas, carts, keys, ATM cards, wristwatches, laptops, magnetic tapes, disks, etc.) must be left in the wet lab room or on the table outside the NMR room
Physical contact
- Don’t lean on the magnet
- Use the stairs provided for the spectrometers when loading the sample
Injury reporting
- Report any injury to Dr. Sukumaran and Dr. Ashan
Biosafety
Safety training
Schedule for a discussion on the following should be made with Mr. Garcia (et. al.)
- Exposure Control Plan
- Metabonomics lab set up in Statler
- Occupational Safety and Health Administration standards Regulations (Standards - 29 CFR) Bloodborne Pathogens
- Questions regarding other aspects of biosafety can be directed to Mr. Leonard Borzynski of EH&S.
Training records
Write a paragraph on the WIKI regarding the date, location, resource person and bullet points of biosafety topics discussed
Vaccination
There are three shots that need to be legally administered to people who are front liners (have direct physical contact with blood and blood products):
- Undergrad/Graduate students: Call University at Buffalo Health Services to schedule an appointment.
- Post-docs: Make sure that you have an active health insurance. Search online for health providers that are affiliated with your current health insurance and schedule an appointment for vaccination. If you have a family doctor, he is also allowed to administer the shots, if he agrees.
Universal safety precautions and lab safety policies
- Prepare 10% (v/v) bleach-water solution before handling serum samples. Keep the solution only for a week. Fill out the form that is posted beside the metabonomics hood for bleach solution preparation indicating the date and name of person who prepared it
- Wash hands with hand soap and water before and after sample preparation
- Wear double purple nitrile gloves whenever you are working with blood and other potentially infectious materials
- Wear full body lab coats at the time
- Wear safety glasses
- Dispose used pipet tips, Eppendorf tubes and gloves that came in contact with serum and serum products in their designated biohazardous waste container. Contact STERICYCLE to schedule for pick up if these containers get filled. Note that the schedules should be made at least 2 days prior to pick up dates.
- Disinfect contaminated safety glasses, lab coats and clothing that were contaminated with serum and serum products by soaking them in a freshly prepared 10% (v/v) bleach-water solution
- Eating and drinking is strictly prohibited inside the NMR lab
- In the event of blood serum spill, cover the spill with freshly prepared bleach solution and let it stand for at least an hour before wiping it off with paper towels. Redisinfect the surface with freshly prepared bleach solution. Throw the used paper towels in the biohardous waste container.
- For NMR tube breakage, recover as much sample as possible from the tube, disinfect the broken tube by soaking it in freshly prepared bleach solution and disinfect the surface. Throw the disinfected glass fragments in the broken glass container
- In the event that the serum sample was broken inside the probe, immediately inform Dr. Sukumaran. Isopropyl alcohol must be used as a cleaner and disinfectant for the probe. Don’ t use bleach. Probe cleaning should only be performed with Dr. Sukumaran. If the probe/spectrometer needs fixing, inform the person who will fix the problem the potentially hazardous nature of the sample before he starts to work
- During data collection, post notes announcing that data acquisition for a potentially hazardous sample is on going
- Wipe the NMR tube with Kimwipes before loading the sample to minimize transfer of dirt to the probe
- If you generate garbage (e.g. used paper towels, Kimwipes, parafilms, or any clutter in general), throw them in regular waste bin
- Clean your working area before leaving the lab
Injury logs and reporting
- In case of fire or magnet quench, call the University police, Dr. Sukumaran and Dr. Szyperski.
- If you cut or puncture yourself while handling potentially biohazardous biofluids, wash the ezposed area with soap and water and seek medical attention from Milliard Filmore Suburban Hospital or from Health Services as soon as possible.
- Report the incident to Dr. Szyperski, Dr. Ashan and Dr. Sukumaran
- You should also contact EH&S (sharps injury reporting)
- Fill out an accident report form (http://hr.buffalo.edu/files/phatfile/Workers_Comp.pdf) and follow the procedures therein
- Record the incident in ‘Sharp Injury Log book’
Additional documents
Chemical hygiene plan
Exposure control plan<span style="font-size: 15px;" />
Sample Storage and Preparation
Data Acquisition
Profiling of other specimens:Non-biofluids