Resonance Assignment/Principles and concepts

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Introduction

This section is intended to introduce a few definitions and concepts in resonance assignment protocols. For in depth description of the process see e.g. Kurt Wütrich's book NMR of Proteins and Nucleic Acids (Wiley, 1986) and John Cavangh's textbook Protein NMR Spectroscopy. Principles and Practice (2nd edition, Elsevier, 2007)

Stable isotope labeling schemes

NMR experiments

Through bond

Through space

Spin systems

Definitions

Identification

Linking spin systems

Matching onto covalent structure