Resonance Assignment/XEASY

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  1. Spin system identification
  2. Backbone resonance assignment
    1. GFT-based spectra
      1. HNCACAB/CABCA(CO)NH
    2. Conventional spectra
      1. HNCACB/CBCA(CO)NH
      2. HNCA/HN(CO)CA
      3. HNCO/HN(CA)CO
      4. NOESY/TOCSY
  3. Side chain resonance assignment
    1. Aliphatic
      1. GFT NMR spectra
        1. (4,3)D GFT HABCAB(CO)NHN
        2. (4,3)D GFT HCCH
      2. Conventional spectra
        1. HAHB(CO)NH
        2. HCCH-COSY
        3. HCCH-TOCSY
        4. Simultaneous NOESY
        5. (H)CC-TOCSY-(CO)NH
        6. H(CC-TOCSY-CO)NH
    2. Aromatic
      1. GFT-based spectra
      2. Conventional spectra
    3. Other
      1. Trp e1 NH and d1 CH
      2. Met e CH3
      3. Asn d2 and Gln e2 NH2
  4. NOESY peak integration