Common NMR experiment sets

Szyperski group

The following experiments are recorded for NC samples

Common experiments	GFT protocol	Conventional protocol	Fractionally 13C-labeled samples
1D 1D 15N T1 1D 15N T2 2D [15N,1H]-HSQC 2D [15N,1H]-HSQC with wide spectral width in 15N (optional) 2D [13C,1H]-HSQC aliphatic 2D [13C,1H]-HSQC aromatic (optional) 2D [13C,1H]-CT-HSQC aliphatic 2D [13C,1H]-CT-HSQC aromatic 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional) 3D HN(CA)CO (optional) 3D (H)CCH-TOCSY 2D HE(CECDCG)CB (optional) 3D 15N/13Cali/13Caro-resolved [1H,1H]-NOESY	(3,2)D GFT HNNCO or 3D HNCO (4,3)D GFT CABCA(CO)NH (4,3)D GFT HNCABCA (4,3)D GFT HABCAB(CO)NH (4,3)D GFT HCCH aliphatic (4,3)D GFT HCCH-COSY aromatic	3D HNCO 3D HN(CA)CO 3D CBCA(CO)NH 3D HNCACB 3D HBHA(CO)NH (H)CCH-COSY aliphatic (H)CCH-COSY aromatic	2D [13C,1H]-CT-HSQC, 28ms delay - optimized for methyl groups 2D [13C,1H]-CT-HSQC, 56ms delay (optional) 2D [13C,1H]-CT-HSQC, 42ms delay (optional) 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional - can also be run for the NC sample)

Data acquisition is taylored for ABACUS approach for resonance assignment. Most 3D spectra are collected with Non-uniform sampling and processed with Multidimensional decomposition,

NH-rooted	CH-rooted (aliphatic)	CH-rooted (aromatic)	Other
2D [15N,1H]-HSQC 3D 15N-NOESY-HSQC 3D HNCO 3D HNCA 3D CBCA(CO)NH 3D HBHA(CO)NH 3D C(CCO)NH-TOCSY (optional) 3D H(CCCO)NH-TOCSY (optional)	2D [13C,1H]-HSQC 2D [13C,1H]-CT-HSQC 3D 13C-NOESY-HSQC 3D (H)CCH-TOCSY 3D H(C)CH-TOCSY	2D [13C,1H]-HSQC 2D [13C,1H]-CT-HSQC (optional) 3D 13C-NOESY-HSQC 3D (H)CCH-TOCSY (optional) 3D H(C)CH-TOCSY (optional)	1D presaturation 1D 15N T1 1D 15N T2 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional) 2D HE(CECDCG)CB (optional) 2D HD(CDCG)CB (optional)