Protein purification

From NESG Wiki
Jump to navigation Jump to search

Samples for NMR

For each protein, we usually make the following samples:

  • [U-15N, 13C]-labeled sample, for resonance assignment and NOE interpretation.

A U-15N and fractional 5%-13C-labeled sample for stereospecific assignment of Val and Leu isopropyl methyl groups based on the method of Neri et. al. (Biochemistry 28, 7510-7516,1989) that we call the NC5 sample.  This sample is prepared using 5% U[1H,13C]-D-glucose and 95% unlabelled glucose in the E. coli minimal  growth media.


The NC5 sample is not [U-5%-13C,U-15N] since it is not uniformly 5% -13C labeled.  The incorporation of 13C in the methyl groups is biosynthetically directed by the E. coli. 

This sample can be called:

           <style><!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0in; margin-bottom:.0001pt; text-align:justify; mso-pagination:widow-orphan; font-size:11.0pt; mso-bidi-font-size:10.0pt; font-family:"Times New Roman"; mso-ascii-font-family:Helvetica; mso-fareast-font-family:"Times New Roman"; mso-hansi-font-family:Helvetica; mso-bidi-font-family:"Times New Roman";} @page Section1 {size:8.5in 11.0in; margin:1.0in 1.25in 1.0in 1.25in; mso-header-margin:.5in; mso-footer-margin:.5in; mso-paper-source:0;} div.Section1 {page:Section1;} --> </style> U-15N, 5% biosynthetically directed 13C (NC5) sample or

            U-15N, fractional 5%-13C-labeled (NC5) sample  

and give the Neri reference.


For RDC measurement:

  • A secondary NC5 sample for RDC measurement.

(Alternatively, for the H-N RDC measurement, a [U-15N]-labeled sample can be used. Rutger's uses a second NC5 sample, since this is all ready being prepared for the stereospecific assignments, and a 13C CHSQC can be compared with the [U-15N, 13C]-labeled sample, to check if the samples are the same.)


For each protein that is a dimer in solution an extra sample may be required in addition to the samples above:

  • 1:1 mixture of natural abundance and [U-15N, 13C]-labeled samples, for intermolecular NOE interpretation.


Typical Rutgers University NMR Buffers

The protein production facility at Rutgers University uses these three NMR buffers for the initial protein screening: [1]:


  • pH 4.5 NMR buffer:  20 mM NH4OAc, 100 mM NaCl, 10 mM DTT, 5 mM CaCl2, 0.02% NaN3, 5% D2O
  • pH 5.5 NMR buffer:  20 mM NH4OAc, 100 mM NaCl, 10 mM DTT, 5 mM CaCl2, 0.02% NaN3, 5% D2O
  • pH 6.5 NMR buffer:  20 mM MES, 100 mM NaCl, 5 mM CaCl2, 10 mM DTT, 0.02% NaN3, 5% D2O


Typical Rutgers University Protein Purification Protocol

E.coli BL21(DE3) are fermented in MJ9 medium [2]. Cell pellets are stored at -20º C.

  1. add 30 ml Lysis buffer to the a frozen cell pellet and thaw.
  2. sonicate in ice bath
  3. centrifuge to remove insoluble part
  4. supernatant is added to an AkTAxpressTM system with a His TrapHP column followed by HiLoad16/60 Superdex 75 gel filtration chromatography.
  5. exchange buffer to screening buffer by concentrating, diluting with new buffer, reconcentrating to 0.3 - 1.0 mM with Amicon ultrafiltration concentrator (Millipore).

Typical University of Toronto (Arrowsmith proteomics NMR lab) NMR Buffers

Standard screening NMR buffers are:

  • a5.0n300zd : 10 mM sodium acetate, pH 5.0, 300 mM NaCl, 10 uM ZnSO4, 10 mM DTT, 0.01 % NaN3, 1 mM benzamidine, 1x inhibitor cocktail, 5% D2O
  • m6.5n450zd: 10 mM MOPS, pH 6.5, 450 mM NaCl, 10 uM ZnSO4, 10 mM DTT, 0.01 % NaN3, 1 mM benzamidine, 1x inhibitor cocktail, 5% D2O
  • t7.7n500zd : 10 mM tris, pH 7.7, 500 mM NaCl, 10 uM ZnSO4, 10 mM DTT, 0.01 % NaN3, 1 mM benzamidine, 1x inhibitor cocktail, 5% D2O


The final NMR buffer for [U-15N, 13C]-labeled sample depends on the protein of interest. all NMR buffers always contain : 0.01 % NaN3, 1 mM benzamidine, 1x inhibitor cocktail.

(1) If the protein has no cysteine in the sequence, do not bother to add ZnSO4 and DTT (Zn ion will just be a nuisance and deuterated DTT is expensive).

Typical University of Toronto Protein Purification Protocol

Required buffers:

i015t8.5n500z : 15 mM imidazole, 10 mM tris, pH 8.5, 500 mM NaCl, 10 uM ZnSO4

i030t8.5n500z : 30 mM imidazole, 10 mM tris, pH 8.5, 500 mM NaCl, 10 uM ZnSO4

i500t8.5n500z : 500 mM imidazole, 10 mM tris, pH 8.5, 500 mM NaCl, 10 uM ZnSO4

1M DTT

1M benzamidine

  1. add 25 mL of i015t8.5n500z into a frozen cell pellet and thaw.
  2. sonicate in ice bath
  3. spin down cell pellet
  4. transfer supernatant into new falcon tube and add 3 mL of nickel beads
  5. rock the tube for at least 30 minutes in cold.
  6. spin down the beads and discard the supernatant
  7. wash the beads with i015t8.5n500z twice and with i030t8.5n500z twice
  8. in the final i030t8.5n500z wash,  pour the beads unto gravity filter column
  9. elute the protein with i500t8.5n500z
  10. add benzamidine, and add DTT
  11. exchange buffer by concentrating, diluting with new buffer, reconcentrating in a vivaspin concentrator.

if it is [U-15N, 13C]-labeled, add step

(9a) put sample in dialysis bag with protease to cut his-tag and dialyse  against  cleavage buffer

(9b) pass the sample through nickel beads again, then follow step (10) above.

References

  1. ↑ Snyder, D, et.al. (2005). “Comparisons of NMR spectral quality and success in crystallization demonstrate that NMR and X-ray crystallography are complementary methods for small protein structure determination.” JACS, 127: 16505-16511. pmid = 16305237
  2. ↑ Jansson M, Li YC, Jendeberg L, Anderson S, Montelione GT, Nilsson B (1996) High-level production of uniformly 15N- and 13C-enriched fusion proteins in Escherichia coli. J Biomol NMR 7: 131-141
Retrieved from "https://nesgwiki.chem.buffalo.edu/index.php?title=Protein_purification&oldid=3772"