Common NMR experiment sets
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Szyperski group
The following experiments are recorded for NC samples
Common experiments
- 1D
- 1D 15N T1
- 1D 15N T2
- 2D [15N,1H]-HSQC
- 2D [15N,1H]-HSQC with wide spectral width in 15N (optional)
- 2D [13C,1H]-HSQC aliphatic
- 2D [13C,1H]-HSQC aromatic (optional)
- 2D [13C,1H]-CT-HSQC aliphatic
- 2D [13C,1H]-CT-HSQC aromatic
- 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
- 3D HN(CA)CO (optional)
- 3D (H)CCH-TOCSY
- 2D HE(CECDCG)CB (optional)
- 3D 15N/13Cali/13Caro-resolved [1H,1H]-NOESY
GFT protocol
- (3,2)D GFT HNNCO or 3D HNCO
- (4,3)D GFT CABCA(CO)NH
- (4,3)D GFT HNCABCA
- (4,3)D GFT HABCAB(CO)NH
- (4,3)D GFT HCCH aliphatic
- (4,3)D GFT HCCH-COSY aromatic
Conventional protocol
- 3D HNCO
- 3D HN(CA)CO
- 3D CBCA(CO)NH
- 3D HNCACB
- 3D HBHA(CO)NH
- (H)CCH-COSY aliphatic
- (H)CCH-COSY aromatic
Fractionally 13C-labeled samples
- 2D [13C,1H]-CT-HSQC, 28ms delay - optimized for methyl groups
- 2D [13C,1H]-CT-HSQC, 56ms delay (optional)
- 2D [13C,1H]-CT-HSQC, 42ms delay (optional)
- 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional - can also be run for the NC sample)