HNCACB/CBCA(CO)NH

Analysis of the HNNCACB/CACB(CO)NHN

In case of NMR data were collected with non-GFT NMR experiments such as HNNCACB/CACß(CO)NHN for backbone assignment, one can treat HNNCACB/CACB(CO)NHN the same as previously described (4,3)GFT HNNCABCA/CABCA(CO)NHN analysis. Simply do the same things as described in analysis of the (4,3)D GFT HNNCABCA and CABCA(CO)NHN spectra:

1. Simulating the intial peak lists;
2. Adjusting peak position in strips of all residues with XEASY;
3. Simulating AutoAssign input files and do the initially backbone assignment by AutoAssign;
4. Maually checking results from AutoAssign and completing the assignment.