Structure Calculation Using CS-RDC-ROSETTA

Introduction

Conventional protein structure determination from NMR data relies heavily on complete or near complete side chain interproton distances.  Obtaining such information can be both labor-intensive and time-consuming.  CS-RDC-Rosetta (Ref. 1) is a new approach aimed at accurately determining protein structures up to 25 kDa using backbone chemical shifts, residual dipolar couplings and backbone amide proton distance, thereby circumventing the side chain assignment process.

Protocols

The publication concerning the CS-RDC-Rosetta approach is currently under review.  The protocols will be released upon publication.

References

1.    Raman, S., Lange, O. et. al. (2009) Science, submitted.