Resonance Assignment/XEASY

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  1. Spin system identification
  2. Backbone resonance assignment
    1. GFT-based spectra
      1. HNCACAB/CABCA(CO)NH
    2. Conventional spectra
      1. HNCACB/CBCA(CO)NH
      2. HNCA/HN(CO)CA
      3. HNCO/HN(CA)CO
      4. NOESY/TOCSY
    3. Side chain resonance assignment
      1. Aliphatic
        1. GFT NMR spectra
          1. (4,3)D GFT HABCAB(CO)NHN
          2. (4,3)D GFT HCCH
        2. Conventional spectra
          1. HAHB(CO)NH
          2. HCCH-COSY
          3. HCCH-TOCSY
          4. Simultaneous NOESY
          5. (H)CC-TOCSY-(CO)NH
          6. H(CC-TOCSY-CO)NH
      2. Aromatic
        1. GFT-based spectra
        2. Conventional spectra
      3. Other
        1. Trp e1 NH and d1 CH
        2. Met e CH3
        3. Asn d2 and Gln e2 NH2
      4. NOESY peak integration
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