Metabonomics SOP

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NMR

Safety Protocols

Sample Storage and Preparation

Serum

Urine

Others:Non-biofluids

Pipetting

Labeling of NMR specimens

Data Acquisition

Profiling of serum

Profiling of urine

Profiling of other specimens:Non-biofluids

For Resonance Assignment

Spiking of Metabolites

Data Archiving

Data Processing

Phase Correction

1D PC

2D PC

Baseline Correction

1D BC

2D BC

Peak Alignment: Recursive Segment-wise

Spectral Quality Assessment (FOM)

Operator Certification?

Targeted Profiling--FOM?

Resonance Assignment

Development of Metabolic Models?

Statistics

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