Bruker Data Processing

Introduction

Processing of spectra at the console is routinely required during data collection.  Here we describe some processing basics on Bruker spectrometers equipped with Topspin 2.0 and higher software.  The user is also refered to the extensive documention that accompany the software (under "Help").

Processing 1D Spectra Using TopSpin

1-Dimensional data sets are processed at the conclusion of the experiment using the command "fp" (Fourier transform and apply phase corrections) or "efp" (also apply exponential multiplication). 

To process a 1D experiment on the fly the user can transfer the current FID using the command "tr", then process as above.

In TopSpin, the phase correction sub-routine is initiated by the clicking the phase correction icon.  Zero and first order phase correction is applied by dragging the left mouse button over the "0" and "1" icons.

Baseline correction of 1D-spectra is done in an interactive fashion using the "bas" command and the "A", "B", "C", "D", and "E" icons.

Processing 2D Spectra Using TopSpin

2-Dimensional data sets are processed at any time during the experiment using the command "xfb".  Check processing parameters (under the processing tab or using "edp") and adjust number of points and apodization functions in each dimension.

Again phasing is accomplished using the phase correction sub-routine.  For 2D spectra rows or columns for phasing are chosen using the mouse and phasing is accomplished as above for each dimension.  The user stores the phase coorections and reprocesses using "xfb".

Automatic basline correction in each dimension is accomplished using the "abs1" and "abs2" commands.