Common NMR experiment sets: Difference between revisions

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(Created page with '== Szyperski group == The following experiments are recorded for NC samples Common experiments *1D *1D 15N T1 *1D 15N T2 *2D [15N,1H]-HSQC *2D [15N,1H]-HSQC with wide spectral…')
 
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== Szyperski group ==
== Szyperski group ==


The following experiments are recorded for NC samples
The following experiments are recorded for NC samples  


Common experiments
Common experiments  


*1D
*1D  
*1D 15N T1
*1D 15N T1  
*1D 15N T2
*1D 15N T2  
*2D [15N,1H]-HSQC
*2D [15N,1H]-HSQC  
*2D [15N,1H]-HSQC with wide spectral width in 15N (optional)<br>
*2D [15N,1H]-HSQC with wide spectral width in 15N (optional)<br>  
*2D [13C,1H]-HSQC aliphatic<br>
*2D [13C,1H]-HSQC aliphatic<br>  
*2D [13C,1H]-HSQC aromatic (optional)<br>
*2D [13C,1H]-HSQC aromatic (optional)<br>  
*2D [13C,1H]-CT-HSQC aliphatic
*2D [13C,1H]-CT-HSQC aliphatic  
*2D [13C,1H]-CT-HSQC aromatic
*2D [13C,1H]-CT-HSQC aromatic  
*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)<br>
*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)<br>  
*3D&nbsp;HN(CA)CO (optional)
*3D&nbsp;HN(CA)CO (optional)  
*3D (H)CCH-TOCSY
*3D (H)CCH-TOCSY  
*2D&nbsp;HE(CECDCG)CB (optional)
*2D&nbsp;HE(CECDCG)CB (optional)  
*3D 15N/13Cali/13Caro-resolved [1H,1H]-NOESY
*3D 15N/13Cali/13Caro-resolved [1H,1H]-NOESY


<br>
GFT protocol
*(3,2)D GFT HN<u>NCO</u> or 3D HNCO<br>
*(4,3)D GFT&nbsp;<u>CABCA</u>(CO)NH<br>
*(4,3)D GFT&nbsp;HN<u>CABCA</u><br>
*(4,3)D <u>GFT&nbsp;HABCAB</u>(CO)NH<br>
*(4,3)D <u>GFT HC</u>CH aliphatic<br>
*(4,3)D <u>GFT&nbsp;HC</u>CH-COSY aromatic
<br>
Conventional protocol


*3D&nbsp;HNCO
*3D&nbsp;HN(CA)CO
*3D&nbsp;CBCA(CO)NH
*3D&nbsp;HNCACB
*3D HBHA(CO)NH
*(H)CCH-COSY aliphatic
*(H)CCH-COSY aromatic


GFT protocol
<br>


*(3,2)D GFT HN<u>NCO</u> or 3D HNCO<br>
Fractionally 13C-labeled samples
*(4,3)D GFT&nbsp;<u>CABCA</u>(CO)NH<br>
 
*(4,3)D GFT&nbsp;HN<u>CABCA</u><br>
*2D [13C,1H]-CT-HSQC, 28ms delay - optimized for methyl groups<br>  
*(4,3)D <u>GFT&nbsp;HABCAB</u>(CO)NH<br>
*2D [13C,1H]-CT-HSQC, 56ms delay (optional)<br>  
*(4,3)D <u>GFT HC</u>CH aliphatic<br>
*2D [13C,1H]-CT-HSQC, 42ms delay (optional)  
*(4,3)D <u>GFT&nbsp;HC</u>CH-COSY aromatic
*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional - can also be run for the NC sample)<br>


<br>


== Arrowsmith group<br> ==


Conventional protocol
Data acquisition is taylored for [[Abacus|ABACUS]] approach for resonance assignment. Most 3D spectra collected with [[Setting_up_non-uniformly_sampled_spectra|Non-uniform sampling]] and processed with [[Processing_non-uniformly_sampled_spectra_with_Multidimensional_Decomposition|Multidimensional decomposition]],


*3D&nbsp;HNCO
*1D presaturation
*3D&nbsp;HN(CA)CO
*1D 15N T1
*3D&nbsp;CBCA(CO)NH
*1D 15N T2
*3D&nbsp;HNCACB
*3D HBHA(CO)NH
*(H)CCH-COSY aliphatic
*(H)CCH-COSY aromatic


*2D [15N,1H]-HSQC<br>
*2D [13C,1H]-HSQC aliphatic<br>
*2D [13C,1H]-HSQC aromatic<br>
*2D [13C,1H]-CT-HSQC aliphatic
*2D [13C,1H]-CT-HSQC aromatic (optional)
*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)<br>
*2D&nbsp;HE(CECDCG)CB (optional)




Fractionally 13C-labeled samples


*2D [13C,1H]-CT-HSQC, 28ms delay - optimized for methyl groups<br>
*3D (H)CCH-TOCSY
*2D [13C,1H]-CT-HSQC, 56ms delay (optional)<br>
*3D 15N/13Cali/13Caro-resolved [1H,1H]-NOESY
*2D [13C,1H]-CT-HSQC, 42ms delay (optional)
*3D&nbsp;HNCO
*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional - can also be run for the NC sample)<br>
*3D&nbsp;HN(CA)CO
*3D&nbsp;CBCA(CO)NH
*3D&nbsp;HNCACB
*3D HBHA(CO)NH
*

Revision as of 21:34, 23 December 2009

Szyperski group

The following experiments are recorded for NC samples

Common experiments

  • 1D
  • 1D 15N T1
  • 1D 15N T2
  • 2D [15N,1H]-HSQC
  • 2D [15N,1H]-HSQC with wide spectral width in 15N (optional)
  • 2D [13C,1H]-HSQC aliphatic
  • 2D [13C,1H]-HSQC aromatic (optional)
  • 2D [13C,1H]-CT-HSQC aliphatic
  • 2D [13C,1H]-CT-HSQC aromatic
  • 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
  • 3D HN(CA)CO (optional)
  • 3D (H)CCH-TOCSY
  • 2D HE(CECDCG)CB (optional)
  • 3D 15N/13Cali/13Caro-resolved [1H,1H]-NOESY


GFT protocol

  • (3,2)D GFT HNNCO or 3D HNCO
  • (4,3)D GFT CABCA(CO)NH
  • (4,3)D GFT HNCABCA
  • (4,3)D GFT HABCAB(CO)NH
  • (4,3)D GFT HCCH aliphatic
  • (4,3)D GFT HCCH-COSY aromatic


Conventional protocol

  • 3D HNCO
  • 3D HN(CA)CO
  • 3D CBCA(CO)NH
  • 3D HNCACB
  • 3D HBHA(CO)NH
  • (H)CCH-COSY aliphatic
  • (H)CCH-COSY aromatic


Fractionally 13C-labeled samples

  • 2D [13C,1H]-CT-HSQC, 28ms delay - optimized for methyl groups
  • 2D [13C,1H]-CT-HSQC, 56ms delay (optional)
  • 2D [13C,1H]-CT-HSQC, 42ms delay (optional)
  • 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional - can also be run for the NC sample)


Arrowsmith group

Data acquisition is taylored for ABACUS approach for resonance assignment. Most 3D spectra collected with Non-uniform sampling and processed with Multidimensional decomposition,

  • 1D presaturation
  • 1D 15N T1
  • 1D 15N T2
  • 2D [15N,1H]-HSQC
  • 2D [13C,1H]-HSQC aliphatic
  • 2D [13C,1H]-HSQC aromatic
  • 2D [13C,1H]-CT-HSQC aliphatic
  • 2D [13C,1H]-CT-HSQC aromatic (optional)
  • 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
  • 2D HE(CECDCG)CB (optional)


  • 3D (H)CCH-TOCSY
  • 3D 15N/13Cali/13Caro-resolved [1H,1H]-NOESY
  • 3D HNCO
  • 3D HN(CA)CO
  • 3D CBCA(CO)NH
  • 3D HNCACB
  • 3D HBHA(CO)NH
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