Common NMR experiment sets: Difference between revisions

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*1D presaturation  
*1D presaturation  
*1D 15N T1  
*1D 15N T1  
*1D 15N T2
*1D 15N T2  
*2D [15N,1H]-HSQC  
*2D [15N,1H]-HSQC  
*2D [13C,1H]-HSQC aliphatic  
*2D [13C,1H]-HSQC aliphatic  
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*2D [13C,1H]-CT-HSQC aliphatic  
*2D [13C,1H]-CT-HSQC aliphatic  
*2D [13C,1H]-CT-HSQC aromatic (optional)  
*2D [13C,1H]-CT-HSQC aromatic (optional)  
*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
*2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)  
*2D HE(CECDCG)CB (optional)
*2D HE(CECDCG)CB (optional)


{!NH-rooted
{!NH-rooted !!CH-rooted !!Aromatic  
!!CH-rooted
|-  
!!Aromatic
|  
|-
*3D 15N-NOESY-HSQC  
|
*3D 15N-NOESY-HSQC
*3D HNCO  
*3D HNCO  
*3D HNCA  
*3D HNCA  
*3D CBCA(CO)NH  
*3D CBCA(CO)NH  
*3D HBHA(CO)NH  
*3D HBHA(CO)NH
||
||  
*3D (H)CCH-TOCSY  
*3D (H)CCH-TOCSY  
*3D H(C)CH-TOCSY  
*3D H(C)CH-TOCSY  
*3D 13C-NOESY-HSQC
*3D 13C-NOESY-HSQC
||
||  
*3D (H)CCH-TOCSY (optional)
*3D (H)CCH-TOCSY (optional)  
*3D H(C)CH-TOCSY (optional)
*3D H(C)CH-TOCSY (optional)  
*3D 13C-NOESY-HSQC
*3D 13C-NOESY-HSQC
|}

Revision as of 21:39, 23 December 2009

Szyperski group

The following experiments are recorded for NC samples

Common experiments

  • 1D
  • 1D 15N T1
  • 1D 15N T2
  • 2D [15N,1H]-HSQC
  • 2D [15N,1H]-HSQC with wide spectral width in 15N (optional)
  • 2D [13C,1H]-HSQC aliphatic
  • 2D [13C,1H]-HSQC aromatic (optional)
  • 2D [13C,1H]-CT-HSQC aliphatic
  • 2D [13C,1H]-CT-HSQC aromatic
  • 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
  • 3D HN(CA)CO (optional)
  • 3D (H)CCH-TOCSY
  • 2D HE(CECDCG)CB (optional)
  • 3D 15N/13Cali/13Caro-resolved [1H,1H]-NOESY


GFT protocol

  • (3,2)D GFT HNNCO or 3D HNCO
  • (4,3)D GFT CABCA(CO)NH
  • (4,3)D GFT HNCABCA
  • (4,3)D GFT HABCAB(CO)NH
  • (4,3)D GFT HCCH aliphatic
  • (4,3)D GFT HCCH-COSY aromatic


Conventional protocol

  • 3D HNCO
  • 3D HN(CA)CO
  • 3D CBCA(CO)NH
  • 3D HNCACB
  • 3D HBHA(CO)NH
  • (H)CCH-COSY aliphatic
  • (H)CCH-COSY aromatic


Fractionally 13C-labeled samples

  • 2D [13C,1H]-CT-HSQC, 28ms delay - optimized for methyl groups
  • 2D [13C,1H]-CT-HSQC, 56ms delay (optional)
  • 2D [13C,1H]-CT-HSQC, 42ms delay (optional)
  • 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional - can also be run for the NC sample)


Arrowsmith group

Data acquisition is taylored for ABACUS approach for resonance assignment. Most 3D spectra collected with Non-uniform sampling and processed with Multidimensional decomposition,

  • 1D presaturation
  • 1D 15N T1
  • 1D 15N T2
  • 2D [15N,1H]-HSQC
  • 2D [13C,1H]-HSQC aliphatic
  • 2D [13C,1H]-HSQC aromatic
  • 2D [13C,1H]-CT-HSQC aliphatic
  • 2D [13C,1H]-CT-HSQC aromatic (optional)
  • 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
  • 2D HE(CECDCG)CB (optional)

{!NH-rooted !!CH-rooted !!Aromatic |- |

  • 3D 15N-NOESY-HSQC
  • 3D HNCO
  • 3D HNCA
  • 3D CBCA(CO)NH
  • 3D HBHA(CO)NH

||

  • 3D (H)CCH-TOCSY
  • 3D H(C)CH-TOCSY
  • 3D 13C-NOESY-HSQC

||

  • 3D (H)CCH-TOCSY (optional)
  • 3D H(C)CH-TOCSY (optional)
  • 3D 13C-NOESY-HSQC

|}