Full probefile calibration: Difference between revisions
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== Full Probefile Calibration == | == Full Probefile Calibration == | ||
This procedure is similar to [[NESG:AutomatedNMRParametersOptimization|Automated Pulse Width Calibration]], but the number of parameters to be optimized is much larger an the required time is much longer. Full calibration is usually needed only after hardware upgrades, e.g. probe or amplifiers. | This procedure is similar to [[NESG:AutomatedNMRParametersOptimization|Automated Pulse Width Calibration]], but the number of parameters to be optimized is much larger an the required time is much longer. Full calibration is usually needed only after hardware upgrades, e.g. probe or amplifiers. | ||
# Insert a concentrated NC-labeled sample of a small protein, preferably in a Varian shigemi with proper filling height. Perform tuning/matching, lock and shim the sample. | #Insert a concentrated NC-labeled sample of a small protein, preferably in a Varian shigemi with proper filling height. Perform tuning/matching, lock and shim the sample. | ||
# Load the <tt>ghn_co</tt> dataset and set the desired parameters - type <tt>man('BioPacklist')</tt> for a list. | #Load the <tt>ghn_co</tt> dataset and set the desired parameters - type <tt>man('BioPacklist')</tt> for a list. | ||
# To start Autocalibration go to '''Setup''' - | #To start Autocalibration go to '''Setup''' -> '''Calibrations''' and select '''Full, using probefile values''' or '''Full, But Use Current 1H Offset and pw''' | ||
<br> -- Main.AlexEletski - 14 Mar 2008 | |||
-- Main.AlexEletski - 14 Mar 2008 | |||
Latest revision as of 15:53, 15 December 2009
Full Probefile Calibration
This procedure is similar to Automated Pulse Width Calibration, but the number of parameters to be optimized is much larger an the required time is much longer. Full calibration is usually needed only after hardware upgrades, e.g. probe or amplifiers.
- Insert a concentrated NC-labeled sample of a small protein, preferably in a Varian shigemi with proper filling height. Perform tuning/matching, lock and shim the sample.
- Load the ghn_co dataset and set the desired parameters - type man('BioPacklist') for a list.
- To start Autocalibration go to Setup -> Calibrations and select Full, using probefile values or Full, But Use Current 1H Offset and pw
-- Main.AlexEletski - 14 Mar 2008