Long-range 15N-1H correlation experiments for histidine rings: Difference between revisions
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== 2D [15N, 1H] long-range HSQC for His rings == | == 2D [15N, 1H] long-range HSQC for His rings == | ||
=== Varian === | === Varian === | ||
This pulse sequence correlates aromatic H-C protons of His with ring nitrogens. | This pulse sequence correlates aromatic H-C protons of His with ring nitrogens. | ||
Use the <tt>rna_WGgNhsqclr</tt> setup macro from BioPack. This macro loads the <tt>rna_WGgNhsqc.c</tt> pulsesequece with parameters for long-range correlations. | Use the <tt>rna_WGgNhsqclr</tt> setup macro from BioPack. This macro loads the <tt>rna_WGgNhsqc.c</tt> pulsesequece with parameters for long-range correlations. | ||
=== Varian (Old) === | === Varian (Old) === | ||
This section is left for historic reference. | This section is left for historic reference. | ||
Use the <tt>gNfhsqc_His.c</tt> pulse sequence. Modified from BioPack <tt>gNfhsqc.c</tt> by adding 90-180-90 composite 180 pulses on 15N and changing decoupling options. | Use the <tt>gNfhsqc_His.c</tt> pulse sequence. Modified from BioPack <tt>gNfhsqc.c</tt> by adding 90-180-90 composite 180 pulses on 15N and changing decoupling options. | ||
* JNH=22.75 Hz, to refocus 1J_NH couplings | *JNH=22.75 Hz, to refocus 1J_NH couplings | ||
* 15N carrier at 200 ppm | *15N carrier at 200 ppm | ||
* 15N spectral width is 200 ppm | *15N spectral width is 200 ppm | ||
* 13C carrier at 125 ppm | *13C carrier at 125 ppm | ||
* 15N decoupling during acquisition is off | *15N decoupling during acquisition is off | ||
* 13C 40 ppm WURST decoupling during acquisition | *13C 40 ppm WURST decoupling during acquisition | ||
* [[NESG:%ATTACHURL%/ | *[[NESG:%ATTACHURL%/gNfhsqc His.tar|gNfhsqc_His.tar]]: long range hsqc package - tested with VnmrJ 2.1B | ||
Contents: | Contents: | ||
*vnmrsys/psglib/gNfhsqc_His.c | |||
* | *vnmrsys/templates/layout/gNfhsqc_His/ | ||
* | *vnmrsys/maclib/gNfhsqc_His | ||
Reference: [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2142369 J. G. Pelton, D. A. Torchia, N. D. Meadow and S. Roseman, ''Protein Sci'' 1993 2: 543-558] | Installation: | ||
*Save the <tt>gNfhsqc_His.tar</tt> file into VnmrJ user home directory (e.g. <tt>/home/vnmr1</tt>). | |||
*Extract with <tt>tar xvf gNfhsqc_His.tar</tt> - existing files will be overwritten! | |||
Reference: [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2142369 J. G. Pelton, D. A. Torchia, N. D. Meadow and S. Roseman, ''Protein Sci'' 1993 2: 543-558] | |||
-- Main.AlexEletski - 29 Oct 2007 | -- Main.AlexEletski - 29 Oct 2007 | ||
Revision as of 20:41, 14 December 2009
2D [15N, 1H] long-range HSQC for His rings
Varian
This pulse sequence correlates aromatic H-C protons of His with ring nitrogens.
Use the rna_WGgNhsqclr setup macro from BioPack. This macro loads the rna_WGgNhsqc.c pulsesequece with parameters for long-range correlations.
Varian (Old)
This section is left for historic reference.
Use the gNfhsqc_His.c pulse sequence. Modified from BioPack gNfhsqc.c by adding 90-180-90 composite 180 pulses on 15N and changing decoupling options.
- JNH=22.75 Hz, to refocus 1J_NH couplings
- 15N carrier at 200 ppm
- 15N spectral width is 200 ppm
- 13C carrier at 125 ppm
- 15N decoupling during acquisition is off
- 13C 40 ppm WURST decoupling during acquisition
- gNfhsqc_His.tar: long range hsqc package - tested with VnmrJ 2.1B
Contents:
- vnmrsys/psglib/gNfhsqc_His.c
- vnmrsys/templates/layout/gNfhsqc_His/
- vnmrsys/maclib/gNfhsqc_His
Installation:
- Save the gNfhsqc_His.tar file into VnmrJ user home directory (e.g. /home/vnmr1).
- Extract with tar xvf gNfhsqc_His.tar - existing files will be overwritten!
Reference: J. G. Pelton, D. A. Torchia, N. D. Meadow and S. Roseman, Protein Sci 1993 2: 543-558
-- Main.AlexEletski - 29 Oct 2007