Protein oligomerization state
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Determinations of a proteins oligomeric state in an NMR sample can sometimes be a challenge. This is especially true in the case of oligomeric mixtures, such as specific or non-specific aggregation.
It is best to measure the oligomerization state of the protein using many different methods in order to best characterize the system.
Within the NESG consortium we the following methods:
- SDS page gel - to confirm the monomer size and to look for SDS resistant oligomers
- Gel filtration
- Gel filtration with in-line static light scattering
- Sedimentation equilibrium
- NMR determined rotational correlation time, Tc.