Protein purification
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Samples for NMR
- For each protein, we usually order the following samples:
* 100% 15N, 100% 13C uniform labeled sample, for resonance assignment and NOE interpretion. * 100% 15N, 5% 13C labeled sample, for stereo specific assignment of VAL and LEU isopropyl moieties.
- For RDC measurements
* A secondary 100% 15N, 5% 13C labeled sample, for RDC measurement.
- For each protein that exist as dimer in solution extra sample may be required in addition to the two samples above
* 1:1 unlabeled and 100% 15N, 100% 13C uniformed labeled mixed sample, for intermolecular NOE interpretation