Resonance Assignment/CARA

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Loading a new template

To start a new structure determination project in CARA you need to load a template. A template is a CARA repository without project that contains definitions for residue types, spectrum type and LUA scripts. You can either load the default template from the templates page of the official CARA web-site, or extract a template from an existing CARA repository.

In CARA click File -> New from template and select the appropriate .cara file. For detailed instructions see http://www.cara.ethz.ch/Wiki/ImportingTemplate


CARA New Template Dialogue.png

CARA Template for GFT spectra BoR54_template.cara: Latest GFT template for BoR54 project

Starting a new project

Normally you would create a new project based on a protein sequence in XEASY format.

If you have the protein sequence in one-letter format you can convert it to XEASY .seq file the following way:

  1. Save the one-letter sequence in a .aa file, e.g. foo.aa
  2. Execute OneLetterFileToSeqFile LUA script from Terminal tab of the main window. Select your .aa file as input and give full path for the output .seq file.

CARA One Letter to Seqfile.png


  1. Introduction
  2. Spin System Identification in 2D 15N-HSQC and 3D HNNCO
  3. Backbone Resonance Assignment
  4. Assignment of HA and HB Resonances with (4,3)D GFT HABCAB(CO)NHN
  5. Side Chain Assignment
    1. Aliphatic side-chain assignment
    2. Aromatic side-chain assignment
    3. Amide side-chain assignment