Resonance Assignment/XEASY: Difference between revisions

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###Conventional spectra  
###Conventional spectra  
####HAHB(CO)NH  
####HAHB(CO)NH  
####HCCH-COSY
####HCCH-COSY  
####[[Side chain assignment with CN-NOESY in XEASY|Simultaneous NOESY]]  
####[[Side chain assignment with CN-NOESY in XEASY|Simultaneous NOESY]]  
##[[Aromatic side chain assignment with Aro-HCCH-COSY in XEASY|Aromatic]]  
##[[Aromatic side chain assignment with Aro-HCCH-COSY in XEASY|Aromatic]]  
##Additional side chain atoms  
##Additional side chain atoms  
###Trp e1 NH and d1 CH  
###[[Trp_e1_and_d1_assignment_with_NOESY|Trp e1 NH and d1 CH]]
###[[Met methyl assignment with NOESY|Met e CH3 ]]  
###[[Met methyl assignment with NOESY|Met e CH3 ]]  
###[[Amide Side Chain assignment with NOESY|Asn d2 and Gln e2 NH2]]  
###[[Amide Side Chain assignment with NOESY|Asn d2 and Gln e2 NH2]]  
#NOESY peak integration
#NOESY peak integration

Revision as of 17:07, 18 December 2009

  1. Introduction
  2. Spin system identification
  3. Backbone resonance assignment
    1. GFT-based spectra
      1. HNCACAB/CABCA(CO)NH
    2. Conventional spectra
      1. HNCACB/CBCA(CO)NH
  4. Side chain resonance assignment
    1. Aliphatic
      1. GFT-based spectra
        1. (4,3)D GFT HABCAB(CO)NHN
        2. (4,3)D GFT HCCH
      2. Conventional spectra
        1. HAHB(CO)NH
        2. HCCH-COSY
        3. Simultaneous NOESY
    2. Aromatic
    3. Additional side chain atoms
      1. Trp e1 NH and d1 CH
      2. Met e CH3
      3. Asn d2 and Gln e2 NH2
  5. NOESY peak integration