HNCACB/CBCA(CO)NH: Difference between revisions

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(Created page with '=== '''Analysis of the HNNCACB/CACB(CO)NHN''' === In case of NMR data were collected with non-GFT NMR experiments such as HNNCACB/CACß(CO)NHN for backbone assignment, one can …')
 
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=== '''Analysis of the HNNCACB/CACB(CO)NHN'''  ===
=== '''Analysis of the HNNCACB/CACB(CO)NHN'''  ===


In case of NMR data were collected with non-GFT NMR experiments such as HNNCACB/CACß(CO)NHN for backbone assignment, one can treat HNNCACB/CACB(CO)NHN the same as previously described (4,3)GFT HNNCABCA/CABCA(CO)NHN analysis. Simply do the same things as described in analysis of the (4,3)D GFT HNNCABCA and CABCA(CO)NHN spectra: <br>  
In case of NMR data were collected with non-GFT NMR experiments such as HNNCACB/CACß(CO)NHN for backbone assignment, one can treat HNNCACB/CACB(CO)NHN the same as previously described [[HNCACAB/CABCA(CO)NH|(4,3)GFT HNNCABCA/CABCA(CO)NHN]] analysis. Simply do the same things as described in analysis of the [[HNCACAB/CABCA(CO)NH|(4,3)D GFT HNNCABCA and CABCA(CO)NHN]] spectra: <br>  


#Simulating the intial peak lists;
#Simulate the intial peak lists  
#Adjusting peak position in strips of all residues with XEASY;
#Adjust the peak positions in strips using [[XEASY|XEASY]]
#Simulating AutoAssign input files and do the initially backbone assignment by AutoAssign;
#Simulate [[AutoAssign|AutoAssign]] input files and do the initial backbone assignment using [[AutoAssign|AutoAssign]]
#Maually checking results from AutoAssign and completing the assignment.
#Maually check results from [[AutoAssign|AutoAssign]] and complete the assignment

Latest revision as of 21:41, 30 November 2009

Analysis of the HNNCACB/CACB(CO)NHN

In case of NMR data were collected with non-GFT NMR experiments such as HNNCACB/CACß(CO)NHN for backbone assignment, one can treat HNNCACB/CACB(CO)NHN the same as previously described (4,3)GFT HNNCABCA/CABCA(CO)NHN analysis. Simply do the same things as described in analysis of the (4,3)D GFT HNNCABCA and CABCA(CO)NHN spectra:

  1. Simulate the intial peak lists
  2. Adjust the peak positions in strips using XEASY
  3. Simulate AutoAssign input files and do the initial backbone assignment using AutoAssign
  4. Maually check results from AutoAssign and complete the assignment