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...with MDD]]). The intention of recoding NUS data is to either speed up the acquisition for high sensitivity experiments and/or concentrated samples, or to increas

[[Media:AlexEletsky_NESG_Wiki_NMRacq.pptx|NESG Wiki: NMR data acquisition (Alexander Eletsky)]] [[Media:HsiauWei-Wiki-Data-Processing.ppt|NESG Wiki: NMR data processing (Hsiau-Wei Lee)]]

=== Acquisition of 1D ¹⁵N T₁ and T₂ on Bruker Spectrometer ...D ¹⁵N relaxation spectra by using the following settings in the acquisition mode:

...<span class="mw-headline">Setting up acquisition of non-uniformly sampled data on Varian spectrometers with BioPack </span> === ...hedule generator once again, otherwise you will not be able to process the data properly.

== NMR Data Acquisition == *NMR data acquisition for protein structure determination

...Arrowsmith group in Toronto|click here for the Arrowsmith protocol for NUS data collection on Bruker instruments]]). Before the Multi-dimensional decomposition can be started, the raw data has to be converted through a number of steps to an appropriate format. The

Processing of spectra at the console is routinely required during data collection.&nbsp; Here we describe some processing basics on Bruker spectro 1-Dimensional data sets are processed at the conclusion of the experiment using the command "f

== '''Acquisition of NMR Data''' == Three types of data provide specific structural information about bound ligands:<br>

==== Considerations for best use of acquisition time<br> ==== The acquisition time for the indirect dimension of this J-mod method can be shorter than th

== Setting up acquisition of non-uniformly sampled 3D spectra on Bruker spectrometers with TopSpin = ...presented in this window. The level of "sparsing" allows to fine tune the acquisition time. It should generally be 30% or more. T2sp parameters dictate the expon

Data acquisition is taylored for [[Abacus|ABACUS]] approach for resonance assignment. Most 3

#When completed, replace the sample in the magnet. Either monitor the real data as it comes in or set up the experiment but run the first increment a numbe ...um and saved in two date-stamped text files in the local users <tt>vnmrsys/data/testlib</tt> directory and also plotted out in graphical form.

Having processed 2D (15N, 1H) HSQC as described in data process, one can do the peak picking and integration of 2D (15N, 1H) HSQC m ...ram [[NESG:PeakintI|PeakintI]].<br> Type command: <br><br> <tt>peakintI ../data/NHsqc6001 nhsqcsa_b.peaks 250 -i -t 2 2 0.1 </tt><br><br> where the <tt>nhs

== NMR Data Collection == #NMR data acquisition for protein structure determination

...quired for the purpose of protein structure validation and refinement. The data can be collected on samples labeled only with ¹⁵N. Obtaining RDC === Data Acquisition and RDC Calculation ===

'''NMR Data Processing &gt;&nbsp; 2D HSQC-TROSY for RDCs Measurement''' <br> ...s applicable to proteins of small and moderate size (10-30 kDa).&nbsp; The acquisition time in the indirect t1 should be at least T2 of nitrogen.&nbsp; The measur

#During data collection, post notes announcing that data acquisition for a&nbsp;potentially hazardous sample is on going ...ozen and stored at -80^oC until needed for&nbsp;cryogenic probes data collection.<br>