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*HTP_NHSQC_short - 60 ms t_1,max, 4 scans *HTP_NHSQC_wide - 40 ms t_1,max, 4 scans, ¹⁵N spectral width 3 times the probe

Isopropyl groups of Val and Leu can be sterospecifically assigned with the help of a 5-10% fractionally 13C-labeled sample. In biosynthesis of amino acids ...[13C,1H] HSQCs with constant-time delays 28 ms (1/J), 42 ms (1.5/J) and 56 ms (2/J)

At Rutgers University, spectra are collected on a MALDI-TOF/TOF ([http://cabm-ms.cabm.rutgers.edu/instruments.html ABI-MDS SCIEX 4800]) in single TOF mode.& <br>Some reasons that the MS mass may not match your calculated mass are:

HDX-MS can be used to identify the disordered N- and C-terminal regions that can b

...> must be given as a multiple of 10 ms for T1 and '''odd''' multiple of 10 ms for T2. *Avoid sampling T2 points beyond 250 ms - it may cause excessive sample heating.

| align="center" | '''¹⁵N ''T''₁ (ms)''' | align="center" | '''¹⁵N ''T''₂ (ms)'''

...ted with a J-modulation delay of 4 ms and iso8.ft2 was collected with a 32 ms delay.&nbsp; These files will be converted to NMRView format using the foll

...</span>There are some situations where the co-centering may not be enough help.<span>&nbsp; </span>Particularly, in regions where there is a limited amoun ...SY (Pascal, 1994) (tm = 100 ms) and 3D 13C-edited aromatic NOESY (tm = 120 ms) spectra, together with dihedral angle constraints computed by TALOS+ (Corn

...ly need to be approximate. In the Arrowsmith group we use 20ms for 13C, 35 ms for 1H and 50ms for 15N. Usually the values are guessed correctly, but you

Here acquisition time is set to 60 ms, because deuterium decoupling should not be used with long duty cycles.

'''Ms. Victoire-Grace Karambizi''' *MS student