User contributions for Admin

8 October 2009

• 14:5814:58, 8 October 2009 diff hist +1,246‎ N Installing and updating BioPack ‎ Created page with '== '''Installing And Updating BioPack''' == You have the options of either perfroming a fresh install or an incremental update. Incremental update is quicker, but it requires …'
• 14:5714:57, 8 October 2009 diff hist +6,346‎ N Pulse width calibration ‎ Created page with '== '''Manual Calibration of Pulse Widths and 1H Carrier Offset''' == === '''Calibration of 1H Carrier Frequency (<tt>tof</tt>)''' === To achieve the best water suppression <tt>…'
• 14:5614:56, 8 October 2009 diff hist +4,215‎ N NESG:AutomatedNMRParametersOptimization ‎ Created page with '== '''Automated Calibration of 90° pulse widths''' == For every sample you should calibrate 90° pulse widths before acquisition. Here we assume that you have BioPack installe…' current
• 14:5414:54, 8 October 2009 diff hist +6,322‎ N NESG:BioPackProbefile ‎ Created page with '== BioPack and Probefile == Here we assume that you have BioPack installed. BioPack uses a probefile to store all important parameters for its library of biomolecular NMR pulse …' current
• 14:5214:52, 8 October 2009 diff hist +12,776‎ N Shimming ‎ Created page with '== '''Sample shimming''' == The shim system is essentially a set of coils with specific field profiles in 3D space. There are three shimming methods: shimming on the lock level,…'
• 14:5014:50, 8 October 2009 diff hist +7,810‎ N Deuterium Lock ‎ Created page with '== '''Deuterium Lock System''' == The lock system keeps the spectrometer operating at a constant net magnetic field. It compensates for the drift of the superconducting magnet's…'
• 14:4914:49, 8 October 2009 diff hist +6,114‎ N Tuning and matching ‎ Created page with '== '''Probe Tuning and Matching''' == NMR probes come in various designs, but the typical triple-resonance HCN probe has two coils and four resonant circuits. The inner observe …'
• 14:4714:47, 8 October 2009 diff hist −266‎ Inserting NMR Sample ‎No edit summary
• 14:4714:47, 8 October 2009 diff hist +2,411‎ N Inserting NMR Sample ‎ Created page with '== '''Sample Loading''' ==<br><br>=== '''Using the sample gauge''' ===<br><br>The optimal volume for NMR samples is 500-600 uL for 5 mm regular NMR tubes and ~300 uL for Shigemi …'
• 14:4514:45, 8 October 2009 diff hist +9,921‎ N NMR Sample Preparation ‎ Created page with '== '''Sample Transfer into NMR Tubes''' == Although our lab seldom produces NMR samples we frequently have to transfer the samples to an NMR tube. According to different requir…'
• 14:3714:37, 8 October 2009 diff hist +5,254‎ N Mass Spectrometry ‎ Created page with '== Compare Mass Spectrometry m/z with Calculated Protein Mass == === Mass Spectrometry Results from SPINE === <br />The mass spectrum chromatogram for many of the purified samp…'
• 14:3514:35, 8 October 2009 diff hist +3,388‎ N NESG:OligomerizationStatus ‎ Created page with '== '''Assessment of Oligomerization Status''' == === '''Gel-filtration Results from SPINE''' === The oligomerization status of each target is noted in SPINE: * Go to the Batch …'
• 14:3314:33, 8 October 2009 diff hist −95‎ NESG:SampleCheck ‎No edit summary
• 14:3314:33, 8 October 2009 diff hist +1,636‎ N NESG:SampleCheck ‎ Created page with '== '''Sample Check''' ==<br><br>=== '''Visual inspection of the sample''' ===<br><br>Once a sample arrives, <br>* Do visual inspection of the sample checking for cloudiness or vi…'
• 14:3014:30, 8 October 2009 diff hist +5,149‎ N NESG:Acquiring an NMR Sample ‎ Created page with '== '''Acquiring an NMR Sample''' == === '''[http://www.nesg.org NESG] Ordering Procedure''' === One should contact the [http://www.nesg.org NESG] protein production centers for…'
• 14:2614:26, 8 October 2009 diff hist −5,970‎ NESG:MasterRecipe ‎No edit summary

7 October 2009

• 16:0816:08, 7 October 2009 diff hist +37‎ N NESG:NESG/WikiWorkshopRecipe ‎ moved NESG:NESG/WikiWorkshopRecipe to NESG:WikiWorkshopRecipe current
• 16:0816:08, 7 October 2009 diff hist 0‎ m Wiki Tree Layout ‎ moved NESG:NESG/WikiWorkshopRecipe to NESG:WikiWorkshopRecipe
• 15:1715:17, 7 October 2009 diff hist +31‎ N NESG:NESG/MasterRecipe ‎ moved NESG:NESG/MasterRecipe to NESG:MasterRecipe current
• 15:1715:17, 7 October 2009 diff hist 0‎ m NESG:MasterRecipe ‎ moved NESG:NESG/MasterRecipe to NESG:MasterRecipe