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12 October 2009
- 13:5213:52, 12 October 2009 diff hist +12,901 N NESG:NOECalibrationCYANA Created page with '== '''NOE Calibration: Finding calibration Parameters for Distance Constraints''' == NOE calibration converts NOESY cross-peak intensities into upper distance limits. A certain …' current
- 13:5113:51, 12 October 2009 diff hist +3,581 N SSAFromFractional13CLabeledSample Created page with '== '''Stereospecific assignments with a 13C Fractionally Labeled Sample''' == Isopropyl groups of Val and Leu can be sterospecifically assigned with the help of a 5-10% fraction…'
- 13:4913:49, 12 October 2009 diff hist +4,364 N TALOS Created page with '== '''Finding Dihedral Angle Constraints with TALOS''' == TALOS (Torsion Angle Likelihood Obtained from Shift and sequence similarity) is a database system for empirical predict…'
- 13:4713:47, 12 October 2009 diff hist +1,188 N NESG:CARAExportingXeasyFiles Created page with '== '''Exporting PeakLists, AtomLists and Sequence from CARA''' == === '''Sequence''' === You can export an XEASY-format sequence file by right-clicking on your project tab and …' current
- 13:4613:46, 12 October 2009 diff hist +1,769 N NESG:CYANAInitFile Created page with '== '''Creating an init.cya file''' == The init.cya is a local initialization file, which is read when cyana starts. It should be located in the directory where CYANA is run. In …' current
9 October 2009
- 17:5717:57, 9 October 2009 diff hist +8,124 N NESG:CARAGeneratingNOESYPeaklists Created page with ' == '''Generating NOESY Peaklists in CARA''' == === '''Simulating Blank NOESY Peaklists (Recommended)''' === It is recommended to start with a blank peaklist. Note that in t…' current
- 17:5517:55, 9 October 2009 diff hist +5,705 N NESG:GeneratingNOESYPeaklists Created page with ' == '''Generating NOESY Initial Peaklist with XEASY/UBNMR''' == === '''Simulating NOESY Peaklists for XEASY''' === Before NOESY analysis, initial NOESY peak lists are simula…' current
- 17:5417:54, 9 October 2009 diff hist +1,785 N NESG:OverviewStructureCalculation Created page with ' == '''Overview of Structure Calculation and Refinement Protocol''' == === Outline === # Simulate initial NOE peaklists (intra and short connectivities), pick additional pea…' current
- 17:5317:53, 9 October 2009 diff hist +16 NESG:CSISecondaryStructurePrediction No edit summary current
- 17:5317:53, 9 October 2009 diff hist +1,400 N NESG:CSISecondaryStructurePrediction Created page with ' == '''Secondary Structure Element Prediction Using CSI''' == You can predict secondary structure from HA, CA, CB and CO chemical shifts by using CSI. See the [http://www.bionm…'
- 17:5117:51, 9 October 2009 diff hist +1,518 N NESG:AssignmentValidation Created page with ' == '''Assignment Validation''' == === '''AVS''' === Once the assignment results were obtained from, it is required to validate the assignments. AutoAssign provides Assignmen…' current
- 17:5017:50, 9 October 2009 diff hist +3,451 N Amide Side Chain Assignment with CARA Created page with ' == '''Amide Side-chain Resonance Assignment''' == You will normally need to have 3 windows open at the same time: # Open aliphatic 2D [13C, 1H]-HSQC with '''Open PolyScope'''…'
- 17:4917:49, 9 October 2009 diff hist +12,041 N Aromatic Side Chain Assignment with CARA Created page with ' == '''Aromatic Side-chain Resonance Assignment''' == Overall, aromatic side-chain assignment is very similar to aliphatic side-chain assignment, only one uses aromatic (H)CCH-…'
- 17:4817:48, 9 October 2009 diff hist +36,749 N Resonance Assignment/CARA/Aliphatic side-chain assignment Created page with ' == '''Aliphatic Side-chain Resonance Assignment''' == === '''Introduction''' === For a short overview of heteronuclear side-chain assignment see this page: http://www.cara.…'
- 17:4617:46, 9 October 2009 diff hist +3,413 N Resonance Assignment/CARA/Backbone assignment GFT Created page with '=== '''HA And HB Assignment in (4,3)D HABCAB(CO)NHN with CARA''' === # Run '''GFT_CreateHABProjSpins''' Lua script to create GFT spins like <nop>CApHA-1 and <nop>CBpHB-1. Known…'
- 17:4517:45, 9 October 2009 diff hist +5,118 N Resonance Assignment/CARA/Backbone assignment Created page with '== '''Backbone Assignment with CARA''' == === '''Picking Spins in (4,3)D GFT HNNCABCA and CABCA(CO)NHN Spectra''' === Open (4,3)D GFT HNNCABCA and CABCA(CO)NHN in SynchroScope …'
- 17:4417:44, 9 October 2009 diff hist +1,480 N Spin System Identification with CARA Created page with '== '''Spin System Identification with CARA''' == Spin system identification in 2D [15N,1H]-HSQC and 3D HNCO # Open a repositiry or create one if it does not exist (see the CARA…'
- 17:4317:43, 9 October 2009 diff hist +6,599 N Side chain assignment with CN-NOESY in XEASY Created page with '== '''Side Chain Assignments Using Simultaneous 15N,13C-resolved(1H,1H)NOESY''' == One can go to the simultaneous 15N,13C-resolved(1H,1H)NOESY directly other than HCCH to finish…'
- 17:3617:36, 9 October 2009 diff hist +1,984 N Met methyl assignment with NOESY Created page with '== '''Met Methyl Resonance Assignment''' == Usually, the Met methyl resonance assignment can be obtained from NOESY, however, in a different manner as described above. The Met m…'
- 17:3417:34, 9 October 2009 diff hist +6,006 N Amide Side Chain assignment with NOESY Created page with '== '''Amide Side-chain Resonance Assignment Using The Simultaneous 15N, 13C-resolved (1H,1H) NOESY''' == The amide side-chain resonance assignment here means the assignment of a…'
- 17:3317:33, 9 October 2009 diff hist −456 Aromatic side chain assignment with Aro-HCCH-COSY in XEASY No edit summary
- 17:3217:32, 9 October 2009 diff hist +3,281 N Aromatic side chain assignment with Aro-HCCH-COSY in XEASY Created page with '<br><br>== '''Aromatic Side-chain Resonance Assignment Using the Aromatic HCCHCOSY Spectrum''' ==<br><br><br>Aromatic (4,3)D HCCH shows signals for aromatic H-C-C-H moeties…'
- 17:3117:31, 9 October 2009 diff hist +13,200 N Side chain assignment with aliphatic (4,3)D HCCH-COSY in XEASY Created page with ' == '''Aliphatic Side-chain Resonance Assignment Using the Aliphatic HCCHCOSY Spectrum''' == The sequence specific 15N /1HN /13CAB resonance assignments obtained as described …'
- 16:3816:38, 9 October 2009 diff hist +4,498 N HA and HB Assignment with GFT in XEASY Created page with '== '''HA And HB Assignments with XEASY/UBNMR''' == HA and HB assignments provide the bridge from BackboneAssignment to the complete [[NESG:SideChainA…'
- 16:3716:37, 9 October 2009 diff hist +16,187 N XEASY Backbone Assignment Created page with '== '''Backbone Assignment with XEASY/UBNMR''' == Sequential backbone and 13CB resonance assignment is associated with mapping of SRDs identified in spin system identification on…'
- 16:3716:37, 9 October 2009 diff hist +5,829 N XEASY Spin system identification Created page with ' == '''Spin System Identification''' == 2D [15N,1H]-HSQC provide pairs of correlated amide 15N / 1HN chemical shifts. They seed '''spin systems''' - spins of individual r…'
- 16:3416:34, 9 October 2009 diff hist +1,120 N NESG:OverviewOfAssignmentProtocol Created page with '== '''Overview of Assignment Protocol''' == The current assignment protocol is developed with mainly using the program XEASY for spectral analsis, which might shift to using CAR…' current
- 15:3915:39, 9 October 2009 diff hist +1 Homodimer Structure Calculation Using CYANA No edit summary
- 15:3815:38, 9 October 2009 diff hist −1 Homodimer Structure Calculation Using CYANA No edit summary
- 15:3515:35, 9 October 2009 diff hist +2 Homodimer Structure Calculation Using CYANA No edit summary
- 15:3415:34, 9 October 2009 diff hist +11 Homodimer Structure Calculation Using CYANA No edit summary
- 15:3315:33, 9 October 2009 diff hist +34 Homodimer Structure Calculation Using CYANA No edit summary
- 15:3015:30, 9 October 2009 diff hist +2,446 Homodimer Structure Calculation Using CYANA No edit summary
- 14:5714:57, 9 October 2009 diff hist +2,273 N Structure Calculation Using CS-Rosetta Created page with '== '''Structure Calculation with CS-Rosetta''' == === '''Original Documentation''' === http://spin.niddk.nih.gov/bax/software/CSROSETTA/index.html === '''Random Coil Index Pre…'
- 14:5614:56, 9 October 2009 diff hist +819 N NESG:PfamProteinFamilies Created page with '== '''PfamProteinFamilies''' == PfamProteinFamilies (http://www.sanger.ac.uk/Software/PfamProteinFamilies/) is a large collection of multiple sequence alignments and hidden Mark…'
- 14:4814:48, 9 October 2009 diff hist +27,639 N NESG:RDCScreening Created page with '-- Main.SonalBansal - 07 Sep 2007 -- Main.DavidParish - 09 Nov 2007 == RDC Screening and Structure Refinement == Residual Dipolar Couplings (RDCs) originate from the anisotrop…' current
- 14:4514:45, 9 October 2009 diff hist +2,279 N Rosetta High Resolution Protein Structure Refinement Protocol Created page with '== '''Rosetta High Resolution Protein Structure Refinement Protocol''' == Before running Rosetta, please make directory with: * rosetta_database (directory) ** paths.txt ** 1…'
- 14:4314:43, 9 October 2009 diff hist +2,459 N Homodimer Structure Calculation Using CYANA Created page with '== '''Dimer Structure Calculation by CYANA''' == CYANA2.1 can handle handle structure calculation: === '''Symmetric Dimers''' === Two new types of restraints have been introd…'
- 14:4314:43, 9 October 2009 diff hist +147 N NESG:StructureDimer Created page with '== '''Structure Calculation for Dimer''' == === Dimer structure calculation by CYANA === -- Main.GaohuaLiu - 09 Nov 2007' current
- 14:4114:41, 9 October 2009 diff hist +2 NESG:PsiBLAST No edit summary current
- 14:4014:40, 9 October 2009 diff hist +2,144 N NESG:PsiBLAST Created page with '==*PSIBLAST* == PSIBLAST iteratively searches one or more protein databases for sequences similar to one or more protein query sequences. PSIBLAST is similar to BLAST except tha…'
- 14:3814:38, 9 October 2009 diff hist +3,913 N NESG:StructureSimilaritySearch Created page with '== '''Structure Similarity Search''' == === '''DALI''' === The [http://www.ebi.ac.uk/dali/ DALI] server is a network service for comparing protein structures in 3D. One submits t…'
- 14:3714:37, 9 October 2009 diff hist +1,518 N NESG:BioInformaticsResources Created page with '== '''Resources for Bioinformatics and Functional Annotation''' == === '''Meta Servers''' === ==== '''Mark-Us (B. Honig, Columbia)''' ==== [http://wiki.c2b2.columbia.edu/honig…'
- 14:3614:36, 9 October 2009 diff hist +12,827 N PDB and BMRB Deposition Created page with '== '''BMRB and PDB Structure Depositions''' == '''NESG SOP For NMR PDB/BMRB Structure Depositions (Dec, 2006)''' ''Note: Truncated coordinates: Researchers used to deposit NMR …'
- 14:3414:34, 9 October 2009 diff hist +11,488 N NESG:AutoStructureRPFScores Created page with '== '''RPF Scores From AutoStructure: Protein NMR structure quality assessment tool''' == This section is taken from the SOP of AutoStructure, please check: * [http://www-nmr.cab…' current
- 14:3314:33, 9 October 2009 diff hist +2,917 N NESG:RunningProcheckNMR Created page with '== '''RunningProcheckNMR''' == [http://www.biochem.ucl.ac.uk/~roman/procheck/procheck.html Procheck] checks the stereochemical quality of a protein structure, producing a number…' current
- 14:3114:31, 9 October 2009 diff hist +2,600 N PSVS Created page with '== '''Running the PSVS Server''' == [http://www-nmr.cabm.rutgers.edu/PSVS/ PSVS] (protein structure validation server) systematically evaluates the quality of protein structures…'
8 October 2009
- 15:4815:48, 8 October 2009 diff hist +571 N NESG:ProFunc Created page with '== '''ProFunc''' == [http://www.ebi.ac.uk/thornton-srv/databases/ProFunc/ ProFunc] is a server offered by the [http://www.ebi.ac.uk/Thornton/ Thornton Group] at EBI. It was deve…' current
- 15:4615:46, 8 October 2009 diff hist +734 N NESG:NMRDataArchiving Created page with '== '''NMR Data Archiving''' == === '''Routine Data Backup''' === Although the Main.HTP server backup data daily, it is required to routinely backup data about project progress. O…'
- 15:4515:45, 8 October 2009 diff hist +1,547 N NESG:PeakDetectionYield Created page with '== '''Determination of Peak Detection Yield''' == It is required to determine the peak detection yield of a NMR spectrum as earlier as possible. Determination of approximate pea…'
- 15:4415:44, 8 October 2009 diff hist +2,030 N NESG:FormatConversion Created page with '== '''NMR Spectra Format Conversion''' == === '''Conversion from Buffalo.NMRPipe Format to XEASY Format''' === After optimizing phase, macro <tt>doit.com</tt> is used to run lin…' current
- 15:4315:43, 8 October 2009 diff hist +3,136 N NESG:AGNuS Created page with '==*Autoproc/AGNuS* == [http://www-nmr.cabm.rutgers.edu/software/autoproc.htm Autoproc/AGNuS] is a suite of programs for the automatic generation of scripts for multidimensional p…' current
- 15:4115:41, 8 October 2009 diff hist +11,024 N NESG:BuffaloNMRPipeFT Created page with '== '''Data Processing Protocol''' == In the <tt>process</tt> directory, go to the directory of the experiment you would like to process and check the README file for instru…' current
- 15:4015:40, 8 October 2009 diff hist +1,660 N NESG:TransferDataFromNMRSpectrometer Created page with '== '''Transfer Data From NMR Spectrometer''' == Go to the <tt>acq</tt> directory under the target directory. Check README for instructions: * This directory will contain ONLY yo…' current
- 15:3915:39, 8 October 2009 diff hist +1,211 N NESG:AutomaticGenerationOfProjectDirectoryTree Created page with '== '''Automatic Generation of Project Directory Tree''' == A set of multi-dimensional heteronuclear GFT NMR experiments (see [http://pubs.acs.org/cgi-bin/searchRedirect.cgi/jacs…' current
- 15:3815:38, 8 October 2009 diff hist +544 N NESG:BuffaloProcessProctocol Created page with '== '''Buffalo's Process Proctocol''' == The current Buffalo processing protocols described here have been developed for NMRpipe with help of Perl Scrips developed by Dr. David P…' current
- 15:3715:37, 8 October 2009 diff hist +835 N NESG:DataProcessing Created page with '== '''Data Processing Overview''' == The current data processing protocols (by using Buffalo scripts or AGNuS) described here are similar as both protocols were designed around …' current
- 15:3515:35, 8 October 2009 diff hist +3,695 N NESG:ExperimentSpecificNMRParameters Created page with '== '''Experiment-specific NMR Parameters''' == It would be very helpful to start with previously optimized parameter set. * Parameters for 2D Experiments for proteins ** 2D 15N…' current
- 15:3415:34, 8 October 2009 diff hist +1,043 N NESG:OtherNMRParametersOptimization Created page with '== Other Parameters == === Amplifier Compression === NMR amplifiers normally exhibit non-linear response at the peak power used for high pulses. This non-linearity has to be ta…' current
- 15:3315:33, 8 October 2009 diff hist +8,840 N NESG:SettingUpMultidimensionalNMRExperiments Created page with '= '''Setting Up Multidimensional NMR Experiments''' = It is recommended to collect different NMR data sets depending on the behavior of the protein, and collect the NOESY spectr…' current
- 15:3115:31, 8 October 2009 diff hist +1,387 N Long-range 15N-1H correlation experiments for histidine rings Created page with '== 2D [15N, 1H] long-range HSQC for His rings == === Varian === This pulse sequence correlates aromatic H-C protons of His with ring nitrogens. Use the <tt>rna_WGgNhsqclr</tt>…'
- 15:3015:30, 8 October 2009 diff hist +628 N NESG:HCCHTocsY Created page with '== Aliphatic 3D (H)CCH-TOCSY == Slightly modified version of the <tt>cch_tocsyA.c</tt> in BioPack. * Corrected small uncompensated initial evolution in 13C t1 dimension * Chang…' current
- 15:2915:29, 8 October 2009 diff hist +1,893 N NESG:43DGFTHCCHCosYali Created page with '== (4,3)D GFT HCCH-COSY Aliphatic == Written by Youlin Xia and H. Atreya. Modified by Main.AlexEletski Requires pre-installed BioPack. [[NESG:%PUBURLPATH%/Buffalo/43DGFTHCCHCo…' current
- 15:2615:26, 8 October 2009 diff hist +2,145 N NESG:43DGFTHabCabconhn Created page with '== (4,3)D GFT HABCAB(CO)NHN == Written by Youlin Xia and H. Atreya. Modified by Main.AlexEletski Requires pre-installed BioPack. [[NESG:%PUBURLPATH%/Buffalo/43DGFTHabCabconhn/…' current
- 15:2415:24, 8 October 2009 diff hist +2,208 N NESG:43DGFTCabCaconhn Created page with '== (4,3)D GFT CABCA(CO)NHN == Written by Youlin Xia and H. Atreya. Modified by Main.AlexEletski Requires pre-installed BioPack. [[NESG:%PUBURLPATH%/Buffalo/43DGFTCabCaconhn/g…' current
- 15:2215:22, 8 October 2009 diff hist +498 N 2D (13C, 1H) HSQC for fractionally 13C-labeled samples Created page with '== 2D [13C, 1H] ct-HSQC for 5% 13C-labeled samples == === Varian === gChsqc_methyl macro will setup a 2D [13C, 1H] constant-time HSQC optimiz…'
- 15:2115:21, 8 October 2009 diff hist +456 N NESG:BuffaloNMRExperimentSet3D Created page with '=== 3D Experiment Set (Buffalo) === <tt>HTP_3D_Setup</tt> creates the following ready-to-run experiments: * 3D HNCO * 3D CBCA(CO)NH * 3D HNCACB * 3D HBHA(CBCACO)NH * 3D aliphat…' current
- 15:2015:20, 8 October 2009 diff hist +276 N NESG:BuffaloNMRExperimentSetGFT Created page with '== '''GFT NMR Experiment Set (Buffalo)''' == * 3D HNCO or (3,2)D HNNCO * (4,3)D CABCA(CO)NHN * (4,3)D HNNCABCA * (4,3)D HABCAB(CO)NHN * (4,3)D aliphatic HCCH-COSY * 3D aliphati…' current
- 15:1915:19, 8 October 2009 diff hist +3,481 N Simultaneous 13C,15N-resolved NOESY Created page with '== Simultaneous 15N-, 13C(ali)-, 13C(aro)-resolved [1H,1H] NOESY == Modified from BioPack <tt>gnoesyCNhsqc.c</tt> by Main.AlexEletski, similar to the previous <tt>cnnoesy.c</tt>…'
- 15:1715:17, 8 October 2009 diff hist +345 N NESG:2DHSQCExperimentSet Created page with '== 2D HSQC Experiment set == Run <tt>HTP_HSQC_setup</tt> to configure the following 2D experiments: * [15N, 1H]-HSQC * [15N, 1H]-HSQC wide 15N spectral width - to see Arg and H…' current
- 15:1515:15, 8 October 2009 diff hist +9,815 N Measuring 15N T1 and T2 relaxation times (Varian) Created page with '== '''Correlation Time and NMR Measurement Time Prediction''' == === '''Experimental Setup''' === Run the <tt>HTP_t1t2_setup</tt> script to setup the following experiments: * 2…'
- 15:1415:14, 8 October 2009 diff hist +860 N NESG:First1DAnd2DHSQC Created page with 'Proton 1D spectrum: # Load the <tt>water</tt> dataset. # Set a large <tt>sw</tt> - you should at least see the range between 15 and -3 ppm for a new protein. Adjust <tt>np</tt> o…' current
- 15:1215:12, 8 October 2009 diff hist +2,131 N NESG:CustomHTPSetupScripts Created page with '== '''Custom NMR Experiment Setup Scripts for VNMRJ''' == <tt>HTP_t1t2_setup</tt>, <tt>HTP_hsqc_setup</tt> and <tt>HTP_3D_setup</tt> configure several workspaces with ready-to-…' current
- 15:1115:11, 8 October 2009 diff hist +9,467 N NESG:Non-UniformSamplingAndMDD Created page with '=== Introduction === This section describes the acquisition and processing of non-uniformly sampled 3D data. The intention is to either speed up the acquisition for high sensiti…' current
- 15:0915:09, 8 October 2009 diff hist +11,274 N Deuterium pulse width calibration and decoupling Created page with '== '''Pulsing and Decoupling on Deuterium Channel''' == The most common application is deuterium decoupling in triple-resonance experiments with deuterated samples. Any time ali…'
- 15:0515:05, 8 October 2009 diff hist +4,778 N Chemical shift referencing Created page with 'This page describes UIPAC-Recommended referencing procedures. See http://www.bmrb.wisc.edu/ref_info/cshift.html and references therein. Also check the information at the CABM web…'
- 15:0415:04, 8 October 2009 diff hist +5,445 N Temperature calibration Created page with '== '''Temperature Calibration''' == Temperature control is crucial to NMR experiments. There is always a small discrepancy between the actual temperature of the sample and the t…'
- 15:0215:02, 8 October 2009 diff hist +3,572 N Conditioning procedure for cryogenic probes Created page with 'The information below is taken from '''Decoupling Noise''' in [http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199919600c.pdf HCN Cold Probe manual] …'
- 15:0115:01, 8 October 2009 diff hist +729 N Rebooting spectrometer console Created page with '== '''Rebooting of Spectrometers''' == From time to time, NMR spectrometers have been through hardware of software failures which leads to an inactive console. In case of that,…'
- 15:0015:00, 8 October 2009 diff hist +812 N Full probefile calibration Created page with '== Full Probefile Calibration == This procedure is similar to Automated Pulse Width Calibration, but the number of parameters to be o…'
- 14:5814:58, 8 October 2009 diff hist +1,246 N Installing and updating BioPack Created page with '== '''Installing And Updating BioPack''' == You have the options of either perfroming a fresh install or an incremental update. Incremental update is quicker, but it requires …'
- 14:5714:57, 8 October 2009 diff hist +6,346 N Pulse width calibration Created page with '== '''Manual Calibration of Pulse Widths and 1H Carrier Offset''' == === '''Calibration of 1H Carrier Frequency (<tt>tof</tt>)''' === To achieve the best water suppression <tt>…'
- 14:5614:56, 8 October 2009 diff hist +4,215 N NESG:AutomatedNMRParametersOptimization Created page with '== '''Automated Calibration of 90° pulse widths''' == For every sample you should calibrate 90° pulse widths before acquisition. Here we assume that you have BioPack installe…' current
- 14:5414:54, 8 October 2009 diff hist +6,322 N NESG:BioPackProbefile Created page with '== BioPack and Probefile == Here we assume that you have BioPack installed. BioPack uses a probefile to store all important parameters for its library of biomolecular NMR pulse …' current
- 14:5214:52, 8 October 2009 diff hist +12,776 N Shimming Created page with '== '''Sample shimming''' == The shim system is essentially a set of coils with specific field profiles in 3D space. There are three shimming methods: shimming on the lock level,…'
- 14:5014:50, 8 October 2009 diff hist +7,810 N Deuterium Lock Created page with '== '''Deuterium Lock System''' == The lock system keeps the spectrometer operating at a constant net magnetic field. It compensates for the drift of the superconducting magnet's…'
- 14:4914:49, 8 October 2009 diff hist +6,114 N Tuning and matching Created page with '== '''Probe Tuning and Matching''' == NMR probes come in various designs, but the typical triple-resonance HCN probe has two coils and four resonant circuits. The inner observe …'
- 14:4714:47, 8 October 2009 diff hist −266 Inserting NMR Sample No edit summary
- 14:4714:47, 8 October 2009 diff hist +2,411 N Inserting NMR Sample Created page with '== '''Sample Loading''' ==<br><br>=== '''Using the sample gauge''' ===<br><br>The optimal volume for NMR samples is 500-600 uL for 5 mm regular NMR tubes and ~300 uL for Shigemi …'
- 14:4514:45, 8 October 2009 diff hist +9,921 N NMR Sample Preparation Created page with '== '''Sample Transfer into NMR Tubes''' == Although our lab seldom produces NMR samples we frequently have to transfer the samples to an NMR tube. According to different requir…'
- 14:3714:37, 8 October 2009 diff hist +5,254 N Mass Spectrometry Created page with '== Compare Mass Spectrometry m/z with Calculated Protein Mass == === Mass Spectrometry Results from SPINE === <br />The mass spectrum chromatogram for many of the purified samp…'
- 14:3514:35, 8 October 2009 diff hist +3,388 N NESG:OligomerizationStatus Created page with '== '''Assessment of Oligomerization Status''' == === '''Gel-filtration Results from SPINE''' === The oligomerization status of each target is noted in SPINE: * Go to the Batch …'
- 14:3314:33, 8 October 2009 diff hist −95 NESG:SampleCheck No edit summary
- 14:3314:33, 8 October 2009 diff hist +1,636 N NESG:SampleCheck Created page with '== '''Sample Check''' ==<br><br>=== '''Visual inspection of the sample''' ===<br><br>Once a sample arrives, <br>* Do visual inspection of the sample checking for cloudiness or vi…'
- 14:3014:30, 8 October 2009 diff hist +5,149 N NESG:Acquiring an NMR Sample Created page with '== '''Acquiring an NMR Sample''' == === '''[http://www.nesg.org NESG] Ordering Procedure''' === One should contact the [http://www.nesg.org NESG] protein production centers for…'
- 14:2614:26, 8 October 2009 diff hist −5,970 NESG:MasterRecipe No edit summary
7 October 2009
- 16:0816:08, 7 October 2009 diff hist +37 N NESG:NESG/WikiWorkshopRecipe moved NESG:NESG/WikiWorkshopRecipe to NESG:WikiWorkshopRecipe current
- 16:0816:08, 7 October 2009 diff hist 0 m Wiki Tree Layout moved NESG:NESG/WikiWorkshopRecipe to NESG:WikiWorkshopRecipe
- 15:1715:17, 7 October 2009 diff hist +31 N NESG:NESG/MasterRecipe moved NESG:NESG/MasterRecipe to NESG:MasterRecipe current
- 15:1715:17, 7 October 2009 diff hist 0 m NESG:MasterRecipe moved NESG:NESG/MasterRecipe to NESG:MasterRecipe