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8 October 2009
- 15:2415:24, 8 October 2009 diff hist +2,208 N NESG:43DGFTCabCaconhn Created page with '== (4,3)D GFT CABCA(CO)NHN == Written by Youlin Xia and H. Atreya. Modified by Main.AlexEletski Requires pre-installed BioPack. [[NESG:%PUBURLPATH%/Buffalo/43DGFTCabCaconhn/g…' current
- 15:2215:22, 8 October 2009 diff hist +498 N 2D (13C, 1H) HSQC for fractionally 13C-labeled samples Created page with '== 2D [13C, 1H] ct-HSQC for 5% 13C-labeled samples == === Varian === gChsqc_methyl macro will setup a 2D [13C, 1H] constant-time HSQC optimiz…'
- 15:2115:21, 8 October 2009 diff hist +456 N NESG:BuffaloNMRExperimentSet3D Created page with '=== 3D Experiment Set (Buffalo) === <tt>HTP_3D_Setup</tt> creates the following ready-to-run experiments: * 3D HNCO * 3D CBCA(CO)NH * 3D HNCACB * 3D HBHA(CBCACO)NH * 3D aliphat…' current
- 15:2015:20, 8 October 2009 diff hist +276 N NESG:BuffaloNMRExperimentSetGFT Created page with '== '''GFT NMR Experiment Set (Buffalo)''' == * 3D HNCO or (3,2)D HNNCO * (4,3)D CABCA(CO)NHN * (4,3)D HNNCABCA * (4,3)D HABCAB(CO)NHN * (4,3)D aliphatic HCCH-COSY * 3D aliphati…' current
- 15:1915:19, 8 October 2009 diff hist +3,481 N Simultaneous 13C,15N-resolved NOESY Created page with '== Simultaneous 15N-, 13C(ali)-, 13C(aro)-resolved [1H,1H] NOESY == Modified from BioPack <tt>gnoesyCNhsqc.c</tt> by Main.AlexEletski, similar to the previous <tt>cnnoesy.c</tt>…'
- 15:1715:17, 8 October 2009 diff hist +345 N NESG:2DHSQCExperimentSet Created page with '== 2D HSQC Experiment set == Run <tt>HTP_HSQC_setup</tt> to configure the following 2D experiments: * [15N, 1H]-HSQC * [15N, 1H]-HSQC wide 15N spectral width - to see Arg and H…' current
- 15:1515:15, 8 October 2009 diff hist +9,815 N Measuring 15N T1 and T2 relaxation times (Varian) Created page with '== '''Correlation Time and NMR Measurement Time Prediction''' == === '''Experimental Setup''' === Run the <tt>HTP_t1t2_setup</tt> script to setup the following experiments: * 2…'
- 15:1415:14, 8 October 2009 diff hist +860 N NESG:First1DAnd2DHSQC Created page with 'Proton 1D spectrum: # Load the <tt>water</tt> dataset. # Set a large <tt>sw</tt> - you should at least see the range between 15 and -3 ppm for a new protein. Adjust <tt>np</tt> o…' current
- 15:1215:12, 8 October 2009 diff hist +2,131 N NESG:CustomHTPSetupScripts Created page with '== '''Custom NMR Experiment Setup Scripts for VNMRJ''' == <tt>HTP_t1t2_setup</tt>, <tt>HTP_hsqc_setup</tt> and <tt>HTP_3D_setup</tt> configure several workspaces with ready-to-…' current
- 15:1115:11, 8 October 2009 diff hist +9,467 N NESG:Non-UniformSamplingAndMDD Created page with '=== Introduction === This section describes the acquisition and processing of non-uniformly sampled 3D data. The intention is to either speed up the acquisition for high sensiti…' current
- 15:0915:09, 8 October 2009 diff hist +11,274 N Deuterium pulse width calibration and decoupling Created page with '== '''Pulsing and Decoupling on Deuterium Channel''' == The most common application is deuterium decoupling in triple-resonance experiments with deuterated samples. Any time ali…'
- 15:0515:05, 8 October 2009 diff hist +4,778 N Chemical shift referencing Created page with 'This page describes UIPAC-Recommended referencing procedures. See http://www.bmrb.wisc.edu/ref_info/cshift.html and references therein. Also check the information at the CABM web…'
- 15:0415:04, 8 October 2009 diff hist +5,445 N Temperature calibration Created page with '== '''Temperature Calibration''' == Temperature control is crucial to NMR experiments. There is always a small discrepancy between the actual temperature of the sample and the t…'
- 15:0215:02, 8 October 2009 diff hist +3,572 N Conditioning procedure for cryogenic probes Created page with 'The information below is taken from '''Decoupling Noise''' in [http://www.varianinc.com/image/vimage/docs/products/nmr/apps/pubs/manuals/0199919600c.pdf HCN Cold Probe manual] …'
- 15:0115:01, 8 October 2009 diff hist +729 N Rebooting spectrometer console Created page with '== '''Rebooting of Spectrometers''' == From time to time, NMR spectrometers have been through hardware of software failures which leads to an inactive console. In case of that,…'
- 15:0015:00, 8 October 2009 diff hist +812 N Full probefile calibration Created page with '== Full Probefile Calibration == This procedure is similar to Automated Pulse Width Calibration, but the number of parameters to be o…'
- 14:5814:58, 8 October 2009 diff hist +1,246 N Installing and updating BioPack Created page with '== '''Installing And Updating BioPack''' == You have the options of either perfroming a fresh install or an incremental update. Incremental update is quicker, but it requires …'
- 14:5714:57, 8 October 2009 diff hist +6,346 N Pulse width calibration Created page with '== '''Manual Calibration of Pulse Widths and 1H Carrier Offset''' == === '''Calibration of 1H Carrier Frequency (<tt>tof</tt>)''' === To achieve the best water suppression <tt>…'
- 14:5614:56, 8 October 2009 diff hist +4,215 N NESG:AutomatedNMRParametersOptimization Created page with '== '''Automated Calibration of 90° pulse widths''' == For every sample you should calibrate 90° pulse widths before acquisition. Here we assume that you have BioPack installe…' current
- 14:5414:54, 8 October 2009 diff hist +6,322 N NESG:BioPackProbefile Created page with '== BioPack and Probefile == Here we assume that you have BioPack installed. BioPack uses a probefile to store all important parameters for its library of biomolecular NMR pulse …' current
- 14:5214:52, 8 October 2009 diff hist +12,776 N Shimming Created page with '== '''Sample shimming''' == The shim system is essentially a set of coils with specific field profiles in 3D space. There are three shimming methods: shimming on the lock level,…'
- 14:5014:50, 8 October 2009 diff hist +7,810 N Deuterium Lock Created page with '== '''Deuterium Lock System''' == The lock system keeps the spectrometer operating at a constant net magnetic field. It compensates for the drift of the superconducting magnet's…'
- 14:4914:49, 8 October 2009 diff hist +6,114 N Tuning and matching Created page with '== '''Probe Tuning and Matching''' == NMR probes come in various designs, but the typical triple-resonance HCN probe has two coils and four resonant circuits. The inner observe …'
- 14:4714:47, 8 October 2009 diff hist −266 Inserting NMR Sample No edit summary
- 14:4714:47, 8 October 2009 diff hist +2,411 N Inserting NMR Sample Created page with '== '''Sample Loading''' ==<br><br>=== '''Using the sample gauge''' ===<br><br>The optimal volume for NMR samples is 500-600 uL for 5 mm regular NMR tubes and ~300 uL for Shigemi …'
- 14:4514:45, 8 October 2009 diff hist +9,921 N NMR Sample Preparation Created page with '== '''Sample Transfer into NMR Tubes''' == Although our lab seldom produces NMR samples we frequently have to transfer the samples to an NMR tube. According to different requir…'
- 14:3714:37, 8 October 2009 diff hist +5,254 N Mass Spectrometry Created page with '== Compare Mass Spectrometry m/z with Calculated Protein Mass == === Mass Spectrometry Results from SPINE === <br />The mass spectrum chromatogram for many of the purified samp…'
- 14:3514:35, 8 October 2009 diff hist +3,388 N NESG:OligomerizationStatus Created page with '== '''Assessment of Oligomerization Status''' == === '''Gel-filtration Results from SPINE''' === The oligomerization status of each target is noted in SPINE: * Go to the Batch …'
- 14:3314:33, 8 October 2009 diff hist −95 NESG:SampleCheck No edit summary
- 14:3314:33, 8 October 2009 diff hist +1,636 N NESG:SampleCheck Created page with '== '''Sample Check''' ==<br><br>=== '''Visual inspection of the sample''' ===<br><br>Once a sample arrives, <br>* Do visual inspection of the sample checking for cloudiness or vi…'
- 14:3014:30, 8 October 2009 diff hist +5,149 N NESG:Acquiring an NMR Sample Created page with '== '''Acquiring an NMR Sample''' == === '''[http://www.nesg.org NESG] Ordering Procedure''' === One should contact the [http://www.nesg.org NESG] protein production centers for…'
- 14:2614:26, 8 October 2009 diff hist −5,970 NESG:MasterRecipe No edit summary
7 October 2009
- 16:0816:08, 7 October 2009 diff hist +37 N NESG:NESG/WikiWorkshopRecipe moved NESG:NESG/WikiWorkshopRecipe to NESG:WikiWorkshopRecipe current
- 16:0816:08, 7 October 2009 diff hist 0 m Wiki Tree Layout moved NESG:NESG/WikiWorkshopRecipe to NESG:WikiWorkshopRecipe
- 15:1715:17, 7 October 2009 diff hist +31 N NESG:NESG/MasterRecipe moved NESG:NESG/MasterRecipe to NESG:MasterRecipe current
- 15:1715:17, 7 October 2009 diff hist 0 m NESG:MasterRecipe moved NESG:NESG/MasterRecipe to NESG:MasterRecipe
- 14:5914:59, 7 October 2009 diff hist −3 Main Page No edit summary
- 14:5914:59, 7 October 2009 diff hist −22 Main Page No edit summary
- 14:5314:53, 7 October 2009 diff hist −42 Main Page No edit summary
- 14:5214:52, 7 October 2009 diff hist +74 Main Page No edit summary
- 14:4714:47, 7 October 2009 diff hist +18 N NESG/ moved NESG/ to NESG over redirect current
- 14:4514:45, 7 October 2009 diff hist +4 N NESG/MasterRecipe Created page with 'test' current
- 14:3814:38, 7 October 2009 diff hist +4 N MasterRecipe Created page with 'sdds' current
6 October 2009
- 14:4914:49, 6 October 2009 diff hist +8 NESG:NESG/SoftWare No edit summary current
5 October 2009
- 17:0917:09, 5 October 2009 diff hist +884 N UBNMR Created page with '== [http://www.nsm.buffalo.edu/Chem/HTP_twiki43/bin/view/NESG/UBNMR UBNMR] == Please clikd for download files: *[http://www.nsm.buffalo.edu/Chem/HTP_twiki43/pub/NESG/UBNMR/UB…'
- 17:0717:07, 5 October 2009 diff hist +172 NESG:NESG/AGNuS No edit summary current
- 17:0017:00, 5 October 2009 diff hist +3,527 N NESG:NESG/AGNuS Created page with '== '''Autoproc/AGNuS''' == [http://www-nmr.cabm.rutgers.edu/software/autoproc.htm Autoproc/AGNuS] is a suite of programs for the automatic generation of scripts for multidimensi…'
- 16:5916:59, 5 October 2009 diff hist −26 NESG:PROSA No edit summary
- 16:5016:50, 5 October 2009 diff hist +1,051 N NESG:PROSA Created page with '== '''PROSA''' == The program package [http://guentert.gsc.riken.go.jp/Software/Prosa.html PROSA] (© by Peter Güntert) is an efficient implementation of the common data proce…'
- 16:4916:49, 5 October 2009 diff hist −738 NESG:NmrPipe No edit summary
- 16:4616:46, 5 October 2009 diff hist +4,845 N NESG:NmrPipe Created page with '== NMRpipe and NMRDraw == *Processing the 1st plane of a 3D spectrum by NMRpipe *#Using the following macro to convert the 1st FID data <br><pre>#!/bin/csh #process2d.s 12/…'
- 16:4416:44, 5 October 2009 diff hist +156 NESG:NESG/SoftWare No edit summary
- 16:2416:24, 5 October 2009 diff hist −9 Wiki Tree Layout No edit summary
- 16:0316:03, 5 October 2009 diff hist +13 NESG:NESG/NMRTechnologyWorkshopAgenda No edit summary current
- 16:0116:01, 5 October 2009 diff hist −2,453 NESG:NESG/NMRTechnologyWorkshopAgenda No edit summary
- 15:4815:48, 5 October 2009 diff hist +65 NESG:NESG/NMRTechnologyWorkshopSummary No edit summary current
- 15:4015:40, 5 October 2009 diff hist +32 NESG:NESG/NMRTechnologyWorkshopSummary No edit summary
- 15:3215:32, 5 October 2009 diff hist −33 NESG:NESG/NMRTechnologyWorkshopSummary No edit summary
- 15:2815:28, 5 October 2009 diff hist +157 N NESG:NESG/PosterAbstract Created page with 'NESG NMR Wiki *Sharing technology in strctural genomics/structureal biology *Focus on protein structure determination by NMR -- AlexEletski - 30 Sep 2009' current
- 15:2715:27, 5 October 2009 diff hist +99 NESG:NESG/NMRWikiDevelopment No edit summary current
- 15:2515:25, 5 October 2009 diff hist −189 NESG:NESG/NMRWikiDevelopment No edit summary
- 15:2215:22, 5 October 2009 diff hist −64 NESG:NESG/NMRWikiDevelopment No edit summary
- 15:2015:20, 5 October 2009 diff hist +39 NESG:NESG/SoftWare No edit summary
- 15:0915:09, 5 October 2009 diff hist −72 NESG:NESG/SoftWare No edit summary
- 15:0815:08, 5 October 2009 diff hist −1,220 NESG:NESG/SoftWare No edit summary
- 14:4814:48, 5 October 2009 diff hist −219 NESG:NESG/SelfTraining No edit summary current
2 October 2009
- 17:0617:06, 2 October 2009 diff hist +5,732 N NESG:NESG/NMRTechnologyWorkshopPresentations Created page with '= 2009 NESG NMR Wiki Workshop Presentations = === 19th July, 2009, Sunday === ===== Pre-session ===== [http://www.nsm.buffalo.edu/Chem/HTP_twiki43/pub/NESG/NMRTechnologyW…' current
- 17:0317:03, 2 October 2009 diff hist −18 Wiki Tree Layout No edit summary
- 17:0317:03, 2 October 2009 diff hist −830 Wiki Tree Layout No edit summary
- 17:0217:02, 2 October 2009 diff hist +8,735 N Wiki Tree Layout Created page with '<div class="twikiToc"> *[http://www.nsm.buffalo.edu/Chem/HTP_twiki43/bin/view/NESG/WikiWorkshopRecipe#HTP_NMR_structure_determination HTP NMR structure determination] **[http://…'
- 16:5616:56, 2 October 2009 diff hist −1,485 NESG:NESG/NMRTechnologyWorkshopSummary No edit summary
- 16:5616:56, 2 October 2009 diff hist +23,645 N NESG:NESG/NMRTechnologyWorkshopSummary Created page with '<div class="patternTopic"><div class="twikiToc"> *[http://www.nsm.buffalo.edu/Chem/HTP_twiki43/bin/view/NESG/NMRTechnologyWorkshopSummary#2009_NESG_Wiki_Workshop_Summarie 2009 NE…'
- 16:5016:50, 2 October 2009 diff hist +3,442 N NESG:NESG/NMRWikiDevelopment Created page with 'September 14, 3 pm ('constituting' phone conference) - organized by Alex Eletsky Summary *All posters at the Keystone meeting shall reference the Wiki *A poster describing th…'
- 16:4516:45, 2 October 2009 diff hist +7,330 N NESG:NESG/NMRTechnologyWorkshopAgenda Created page with '== NMR Technology Workshop Topics == Please see the agenda as a Word file: [http://www.nsm.buffalo.edu/Chem/HTP_twiki43/pub/NESG/NMRTechnologyWorkshopAgenda/wiki-workshop-agend…'
- 16:3316:33, 2 October 2009 diff hist −1,621 NESG:NESG/SoftWare No edit summary
- 16:3216:32, 2 October 2009 diff hist +6,086 N NESG:NESG/SoftWare Created page with '<div class="patternTopic"><div class="twikiToc"> *[http://www.nsm.buffalo.edu/Chem/HTP_twiki43/bin/view/NESG/SoftWare#SoftWare_for_NMR_Structure_Deter SoftWare for NMR Structure …'
- 16:2516:25, 2 October 2009 diff hist −41 NESG:NESG/SelfTraining No edit summary
- 16:2516:25, 2 October 2009 diff hist +57 NESG:NESG/SelfTraining No edit summary
- 16:2416:24, 2 October 2009 diff hist +5 NESG:NESG/SelfTraining No edit summary
- 16:2316:23, 2 October 2009 diff hist +909 N NESG:NESG/SelfTraining Created page with '<div class="patternTopic"><div class="twikiToc"> *[http://www.nsm.buffalo.edu/Chem/HTP_twiki43/bin/view/NESG/SelfTraining#Self_Training Self Training ] **[http://www.nsm.buffalo…'
- 16:1516:15, 2 October 2009 diff hist −49 NESG:MasterRecipe No edit summary
- 16:1316:13, 2 October 2009 diff hist +49 NESG:MasterRecipe No edit summary
- 16:1016:10, 2 October 2009 diff hist −1,643 NESG:MasterRecipe No edit summary
- 16:0116:01, 2 October 2009 diff hist −17 NESG:MasterRecipe No edit summary
- 15:5715:57, 2 October 2009 diff hist +16,840 N NESG:MasterRecipe Created page with '<div class="twikiToc"> *[http://www.nsm.buffalo.edu/Chem/HTP_twiki43/bin/view/NESG/MasterRecipe#Outline_of_HTP_Master_Recipe Outline of HTP Master Recipe] **[http://www.nsm.buff…'