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**[[1D screening|<sup>1</sup>H 1D screening]] | **[[1D screening|<sup>1</sup>H 1D screening]] | ||
**[[Nhsqc screen|Initial [<sup>15</sup>N,<sup>1</sup>H] HSQC]]<br> | **[[Nhsqc screen|Initial [<sup>15</sup>N,<sup>1</sup>H] HSQC]]<br> | ||
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== NMR Data Acquisition == | |||
{| cellspacing="1" | |||
|- valign="top" | |||
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*Routine operation | |||
**[[NMR sample tubes]] | |||
**[[NMR Sample Preparation]] | |||
**[[Inserting NMR Sample]] | |||
**Tuning and matching | |||
**[[Deuterium Lock]] | |||
**[[Shimming]] | |||
**[[Pulse width calibration]] | |||
**[[Temperature calibration]] | |||
**[[Chemical shift referencing]] | |||
*Advanced operation | |||
**[[Deuterium pulse width calibration and decoupling]] | |||
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*NMR data acquisition for protein structure determination | |||
**[[Common NMR experiment sets]] | |||
**[[NMR experiment setup scripts for VNMRJ|Custom NMR experiment setup scripts for VNMRJ]] | |||
**[[Estimation of rotational correlation time]] | |||
**[[Estimation of measurement time]] | |||
**[[Simultaneous 13C,15N-resolved NOESY]] | |||
**[[2D (13C, 1H) HSQC for fractionally 13C-labeled samples|2D [13C, 1H]-HSQC for fractionally 13C-labeled samples]] | |||
**[[Long-range 15N-1H correlation experiments for histidine rings]] | |||
*Non-uniform sampling (NUS) | |||
**[[Setting up non-uniformly sampled spectra/NUS guide for Varian|NUS - Varian]] | |||
**[[Setting up non-uniformly sampled spectra/NUS guide for Bruker according to Arrowsmith group in Toronto|NUS - Bruker]] | |||
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*Maintenance (VARIAN) | |||
**[[Installing and updating BioPack]] | |||
**[[Full probefile calibration]] | |||
**[[Rebooting spectrometer console]] | |||
**[[Conditioning procedure for cryogenic probes]] | |||
|} | |} | ||
Revision as of 19:19, 2 December 2009
Welcome to the NESG Wiki!
For a more linear view of the contents see Wiki Tree Layout
Protein sample production
NMR Data Acquisition
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Resonance Assignment
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Structure Calculation and Validation
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