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Please leave your comments/suggestion at the bottom of this page  
Please leave your comments/suggestion at the bottom of this page  


<br>
<br>  


= HTP NMR structure determination  =
= HTP NMR structure determination  =


== Compile Information on Protein Target  ==
== Protein Target Selection, Sample Preparation, and Initial Screening ==


#Competition report
#[[Target selection|NESG target selection]] <br>
#Oligomerization state
#[[DNA cloning protocols|DNA cloning protocols]]<br>
#[[MassSpectrometry|Mass spectrum]]  
#[[Protein purification|Protein expression and purification protocols]]&nbsp;<br>
#Disorder prediction
#Sample Optimization
#Pfam entry
##[[Construct optimization]]
#HSQC and protein concentration
##[[Buffer optimization]]
##[[Cofactor optimization]]
#Initial protein analysis
##[[SDS page gel]]
##[[Protein concentration|Protein concentration measurements]]
##[[Oligomerization Status|Assessment of Oligomerization Status]]
###[[Gel filtration and light scattering|gel-filtration and light scattering]]
###[[NMR determined Rotational correlation time]]
##[[MassSpectrometry|Mass spectrum]]  
##[[NMR screening]]
<br>


== NMR Data Collection  ==
== NMR Data Collection  ==
Line 30: Line 40:
##Tuning and matching  
##Tuning and matching  
##[[Deuterium Lock]]  
##[[Deuterium Lock]]  
##Gradient shimming
##[[Shimming]]
##Shimming with the CHCl3 lineshape sample
##[[Pulse width calibration]]  
##[[Pulse width calibration]]  
##[[Temperature calibration]]  
##[[Temperature calibration]]  
##[[Chemical shift referencing]]
##[[Chemical shift referencing]]  
#Advanced operation  
#Advanced operation  
##[[Deuterium pulse width calibration and decoupling]]
##[[Deuterium pulse width calibration and decoupling]]  
#NMR data acquisition for protein structure determination  
#NMR data acquisition for protein structure determination  
##Custom NMR experiment setup scripts for VNMRJ  
##[[Common NMR experiment sets]]
##[[NMR experiment setup scripts for VNMRJ|Custom NMR experiment setup scripts for VNMRJ]]
##1D 1H NMR spectra and 2D [15N, 1H]-HSQC  
##1D 1H NMR spectra and 2D [15N, 1H]-HSQC  
##Estimation of correlation time of overall tumbling
##[[Estimation of rotational correlation time]]
##Estimation of measurement time  
##[[Estimation of measurement time]]
##NMR experiments for spin system identification  
##NMR experiments for spin system identification  
##2D and 3D NOESY  
##2D and 3D NOESY experiments
###[[Simultaneous 13C,15N-resolved NOESY]]
##Double and triple NMR experiments  
##Double and triple NMR experiments  
###3D CBCA(CO)NH and HNCACB  
###3D CBCA(CO)NH and HNCACB  
Line 54: Line 65:
###H(C)CH  
###H(C)CH  
###H(C)CH-TOCSY  
###H(C)CH-TOCSY  
###(4,3)D HCCH
###(4,3)D HCCH  
##Other NMR experiments  
##Other NMR experiments  
###2D [13C, 1H]-HSQC for 5% 13C-labeled samples  
###[[2D (13C, 1H) HSQC for fractionally 13C-labeled samples|2D [13C, 1H]-HSQC for fractionally 13C-labeled samples]]
###2D [15N, 1H]-long-range-HSQC for determination of histidine protomer state  
###[[Long-range 15N-1H correlation experiments for histidine rings]] - determination of histidine isoprotomer state  
###MEXICO  
###MEXICO  
###CLEANEX  
###CLEANEX  
###H-D exchange experiment  
###H-D exchange experiment  
###15N spin relaxation parameters
###15N spin relaxation parameters  
#Advanced problems for data collection  
#Advanced problems for data collection  
##Setting up non-uniformly sampled spectra  
##[[Setting up non-uniformly sampled spectra]]
###Guide for Varian/BioPack  
###[[Setting up non-uniformly sampled spectra/NUS guide for Varian|Guide for Varian/BioPack]]
###Guide for Bruker according to Arrowsmith group
###[[Setting up non-uniformly sampled spectra/NUS guide for Bruker according to Arrowsmith group in Toronto|Guide for Bruker/Topspin according to Arrowsmith group]]
#Maintenance  
#Maintenance  
##VARIAN  
##VARIAN  
###Installing and updating <span class="twikiNewLink">BioPack</span>
###[[Installing and updating BioPack]]
###Full Probefile calibration  
###[[Full probefile calibration]]
###Rebooting the console  
###[[Rebooting spectrometer console]]
###Cryoprobe conditioning
###[[Conditioning procedure for cryogenic probes]]
##BRUKER
##BRUKER


== NMR Data Processing  ==
== NMR Data Processing  ==


#General Priciples and Concepts
#Processing Procedures for Routine Experiments<br>
##Fourier transformation
##NMRPipe
###Zero-filling
###[[Brief description of philosophy, commands, and functions of NMRPipe|Brief description of philosophy, commands, and functions of NMRPipe]]
###Apodization
###[[Routine 2D Experiment|Routine Processing Procedure for 2D Experiment]]<sup></sup>
###Phasing
###[[Routine Processing Procedure for 3D 15N and 13C-edited Experiments|Routine Processing Procedure for 3D <sup>15</sup>N and <sup>13</sup>C-edited Experiments]]
###Linear prediction
###[[HSQCTROSY RDC Measurement|2D ]]<sup>[[HSQCTROSY RDC Measurement|15]]</sup>[[HSQCTROSY RDC Measurement|N HSQC-TROSY experiment for RDC measurement]]
###G-matrix Fourier transformation (GFT)
###[[Jmodulation Experiment RDC|2D J-modulation experiment for RDC measurement]]
##Alternatives to Fourier transformation
###[[Processing NMR spectra with UB PERL scripts|Routine processing of spectra using Buffalo-developed PERL&nbsp;scripts]]
###Maximum entropy reconstruction
##[[Processing NMR spectra with PROSA|PROSA]]
###MDD reconstruction
##[[Bruker Data Processing|TOPSPIN]]
###...
##[[AGNuS/AutoProc|AUTOPROC]]
#Practical Aspects
##[[UBNMR|UBNMR]]
##NMRPIPE
#Processing Procedures for Alternative Data Collection Methods
###<font color="#000000">General information</font>
##G-Matrix Fourier transformation (GFT)
###<font color="#0000ff">Buffalo's Processing Protocol using NMRpipe</font>
##[[Processing non-uniformly sampled spectra with Multidimensional Decomposition|Processing non-uniformly sampled spectra with Multidimensional Decomposition]]
##PROSA
#Spectra Format Conversion
##TOPSPIN
##[[Spectra Format Conversion from NMRPipe Data|NMRPipe processed data conversion to Sparky, CARA, XEASY, and NMRViewJ]]
##AGNUS/AUTOPROC
##TOPSPIN processed data conversion to Sparky, CARA, XEASY, and NMRViewJ<br>
##UBNMR
##Spectral format conversion
 
<br>


== Resonance Assignment  ==
== Resonance Assignment  ==


This chapter would focus on individual data analysis and resonance assignment packages, as most people stick to a particular software for entire structure determination projects.  
This chapter focuses on data analysis and resonance assignment packages, as most people stick to a particular software for entire structure determination projects.  


<br>
<br>  


#Principles and concepts  
#[[Resonance Assignment/Principles and concepts|Principles and concepts]]
##Stable isotope labeling schemes
#[[Resonance Assignment/Practical aspects|Practical aspects]]
##NMR experiments
###Through-bond
###Through space
##Spin systems
###Definitions
###Identification
###Linking spin systems
###Matching onto covalent structure
#Practical aspects  
##Semi-automated protocols  
##Semi-automated protocols  
###CARA  
###[[Resonance Assignment/CARA|CARA]]
###CCPN ANALYSIS
####[[Spin System Identification with CARA|Spin System Identification in 2D 15N-HSQC and 3D HNNCO]]
###NMRVIEW
####[[Backbone Assignment with CARA|Backbone Resonance Assignment]]
###[[Sparky|Sparky]]  
####[[HA and HB Assignment with CARA|Assignment of HA and HB Resonances with (4,3)D GFT HABCAB(CO)NHN]]
###XEASY  
####Side Chain Assignment
####[[SpinSystemIdentification|Spin system identification]]  
#####[[Aliphatic Side Chain Assignment with CARA|Aliphatic side-chain assignment]]
####Backbone resonance assignment'''<br>'''
#####[[Aromatic Side Chain Assignment with CARA|Aromatic side-chain assignment]]
#####[[Amide Side Chain Assignment with CARA|Amide side-chain assignment]]
###[[Sparky]]
###[[Resonance Assignment/XEASY|XEASY]]
####[[XEASY Introduction|Introduction]]
####[[XEASY Spin system identification|Spin system identification]]  
####Backbone resonance assignment'''<br>'''  
#####GFT-based spectra  
#####GFT-based spectra  
######HNCACAB/CABCA(CO)NH
######[[HNCACAB/CABCA(CO)NH]]
#####Conventional spectra  
#####Conventional spectra  
######HNCACB/CBCA(CO)NH  
######[[HNCACB/CBCA(CO)NH]]<br>
######HNCA/HN(CO)CA
######HNCO/HN(CA)CO
######NOESY/TOCSY
####Side chain resonance assignment  
####Side chain resonance assignment  
#####Aliphatic  
#####Aliphatic  
######GFT NMR spectra  
######GFT-based spectra  
#######(4,3)D GFT HABCAB(CO)NHN  
#######[[HA and HB Assignment with GFT in XEASY|(4,3)D GFT HABCAB(CO)NHN]]
#######(4,3)D GFT HCCH
#######[[Side chain assignment with aliphatic (4,3)D HCCH-COSY in XEASY|(4,3)D GFT HCCH]]
######Conventional spectra  
######Conventional spectra  
#######HAHB(CO)NH  
#######HAHB(CO)NH  
#######HCCH  
#######HCCH-COSY<br>
#######HCCH-TOCSY
#######[[Side chain assignment with CN-NOESY in XEASY|Simultaneous NOESY]]
#######simultaneous NOESY  
#####[[Aromatic side chain assignment with Aro-HCCH-COSY in XEASY|Aromatic]]
#######(H)CC-TOCSY-(CO)NH
#######H(CC-TOCSY-CO)NH
#####Aromatic  
######GFT-based spectra
######Conventional spectra
#####Other  
#####Other  
######Trp e1 NH and d1 CH  
######Trp e1 NH and d1 CH  
######Met e CH3  
######[[Met methyl assignment with NOESY|Met e CH3 ]]
######Asn d2 and Gln e2 NH2
######[[Amide Side Chain assignment with NOESY|Asn d2 and Gln e2 NH2]]
#####NOESY peak integration
####NOESY peak integration  
##Automated protocols  
##Automated protocols  
###AUTOASSIGN
###[[AutoAssign|AutoAssign]]
###CLOUDS/ABACUS  
###[[AutoAssign WebServer|AutoAssign server]]
###PINE
###[[Abacus|ABACUS]]
###[[The PINE Server|PINE server]]
##Validation of resonance assignment  
##Validation of resonance assignment  
##Depositing chemical shifts
###[[AVS|Assignment validation suite (AVS)]]
###[[LACS|Linear analysis of chemical shift (LACS)]]
##[[PDB and BMRB Deposition#Preparing_files_for_BMRB_depostion|Depositing chemical shifts]]


<br>
<br>
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== Structure Calculation and Validation  ==
== Structure Calculation and Validation  ==


#Principles and concepts  
#[[Structure Calculation and Validation|Principles and concepts]]
##Constraints
###Distance (NOE, H-bond, distance calibration, NOE averaging, stereospecific assignments, PRE)
###Dihedral angle (J-coupling, chemical shifts)
###Orientational constraints (RDC, PRE)
##Advanced approaches to derive structural information
###SAXS
##Computational methods
##Assignment of NOESY peaklists and structure calculation
##Structure validation and quality assessment
#Practical aspects  
#Practical aspects  
##Structure calculation  
##Structure calculation  
###CYANA  
###CYANA  
####Getting started (init.cya, sequence, chemical shifts, peaks, macros/scripts, homodimers, cis-PRO, HIS protomer)
####[[CYANA|Getting started]]
####Dihedral angle constraints (link to FOUND, CASHIFT, link to TALOS tutorial, J-couplings, converting TALOS to [http://www.nmr2.buffalo.edu/enter/NMRWiki/index.php/NESG_NMR_WIKI:NESG/CYANA CYANA])
####Distance constraints (link to FOUND, various methods for NOE -&gt; distance conversion, sum/average, stereospecific assignments, distance modification)
####Orientational restraints (RDCs) (link to REDCAT/PALES,FINDTENSOR, .rdc file, adding ORI to PDB file)
####[[FOUND|FOUND]]  
####[[FOUND|FOUND]]  
####[[NoeassignCYANARun|Automated NOESY peaklist assignment (NOEASSIGN)]]  
####[[TALOS|TALOS]]  
####[[ManualCYANARun|Manual structure calculation]]  
####[[GLOMSA|GLOMSA]]  
####[[DimersByCYANA|Homodimer structure calculation]]  
####[[NOE Calibration Using CYANA|NOE calibration]]  
####[[GLOMSA|GLOMSA]]
####[[Manual Structure Calculation Using CYANA|Manual structure calculation]]  
###AUTOSTRUCTURE
####[[Automated NOESY Assignment Using CYANA|Automated NOESY assignment and structure calculation]]
####Getting started (control file, GUI, cis-PRO)  
####[[Structure Calculation With RDC's Using CYANA|Structure calculation with residual dipolar couplings]] (link to REDCAT/PALES,FINDTENSOR, .rdc file, adding ORI to PDB file)  
####Dihedral angle constraints (HYPER)
####[[Homodimer Structure Calculation Using CYANA|Homodimer structure calculations]][[Homodimer Structure Calculation Using CYANA|<br>]]
####Distance constraints
###AutoStructure
####Automated NOESY peaklist assignment (NOEASSIGN)
####[[AutoStructure|Getting started]]
####CYANA run  
####[[CYANA Structure Calculations Using AutoStructure|CYANA run]]
####XPLOR run  
####[[XPLOR Structure Calculations Using AutoStructure|XPLOR run]]
####RPF/DP scores  
####[[Analyzing AutoStructure Output Directories|Analyzing the output]]
####Structure calculation using AS-DP
####[[RPF Analysis|RPF/DP scores]]
###CNS
####[[Structure Calculation Using AS-DP|Structure calculation using AS-DP]]
####Getting started
###"Consensus"&nbsp;Approaches
####Preparation of input files for CNS
####[[Overview of Consensus Runs|Overview of Consensus runs]]
####Refinement in explicit water bath
####[[Finding Consensus NOE Assignments|Finding Consensus NOE assignments]]
###XPLOR
####[[Validation of Consensus Run|Validation of Consensus runs]]
###CS-ROSETTA  
###[[Structure Calculation Using CS-Rosetta|CS-ROSETTA]]
###CS-DP ROSETTA  
###[[Structure Calculation Using CS-DP ROSETTA|CS-DP ROSETTA]]
###RDC-ROSETTA  
###[[Structure Calculation Using CS-RDC-ROSETTA|CS-RDC-ROSETTA]]
###[[RDC-assisted dimer structure determination|RDC-assisted dimer structure calculation<br>]]
###[[RDC-Assisted Dimer Structure Determination|RDC-assisted dimer structure calculation]]<br>  
###Special topics  
###Special topics  
####[[Small Ligands|Protein-Ligand Complexes]]  
####[[Protein-Ligand Complexes|Protein-Ligand complexes]]  
####Metal Ions  
####[[Working With Metal Ions|Metal ions]]
####Dimers  
####[[Working With Dimers|Dimers]]
####PRE constraints
####[[Residual Dipolar Couplings in Structure Refinement|Residual Dipolar Couplings]]
##"Consensus" approaches
####[[REDCAT|REDCAT]]&nbsp;and [[REDCRAFT|REDCRAFT]]
###[[OverviewOfConsensusRuns|Overview]]  
####[[Paramagnetic Constraints in Structure Determination|Paramagnetic constraints]]
###[[FindingConsensusNOEAssignments|Finding Consensus NOE Assignments]]  
##Structure Refinement
###[[ValidationOfConsensusRun|Validation of Consensus Runs]]
###[[Structure Refinement Using CNS Energy Minimization With Explicit Water|CNS refinement]]  
###[[Structure Refinement Using XPLOR-NIH|XPLOR-NIH refinement]]  
###[[Rosetta High Resolution Protein Structure Refinement Protocol|ROSETTA refinement]]  
##Validation and deposition  
##Validation and deposition  
###[[PdbStat|PdbStat]]
###[[PSVS|PSVS]]  
###[[PSVS|PSVS]]  
###[[AutoStructureRPFScores|RPF analysis]]  
###[[RPF Analysis|RPF analysis]]  
###[[MolProbity server|MolProbity server]]  
###[[MolProbity Server|MolProbity server]]  
###[[PdbStat|PdbStat]]
###[[PDB and BMRB Deposition|PDB and BMRB deposition]]  
###[[PdbBmrbDeposition|PDB and BMRB Deposition]]  
###[[ADIT-NMR|ADIT-NMR]]  
###[[ADIT-NMR|ADIT-NMR]]  
###[[HarvestDB|HarvestDB]]  
###[[HarvestDB|HarvestDB]]  

Latest revision as of 20:51, 14 December 2009

This outline of the NESG NMR Wiki is designed to expand on the existing "Master Recipe" and should serve as an experience harvesting tool.

  • It has a rather broad coverage to facilitate long-tewrm growth and development. Aditional compact aggregator pages may be needed to pesent specific information concisely.
  • There would be separate webs within the wiki: Public(or Main), NESG, and member lab webs. Most common knowlege topics should be public, unless they are specific to NESG
  • We assume that the target audience has some knowledge about NMR and protein structure determination, but make the content useful for training
  • "Resonance Assignment" and "Structure Determination" chaptes would focus on individual software packages. The XEASY resonance assignment tree, as the most complete, would serve as a template for other software.
  • Most chapters should include a "general principles" section.

Please leave your comments/suggestion at the bottom of this page


HTP NMR structure determination

Protein Target Selection, Sample Preparation, and Initial Screening

  1. NESG target selection
  2. DNA cloning protocols
  3. Protein expression and purification protocols 
  4. Sample Optimization
    1. Construct optimization
    2. Buffer optimization
    3. Cofactor optimization
  5. Initial protein analysis
    1. SDS page gel
    2. Protein concentration measurements
    3. Assessment of Oligomerization Status
      1. gel-filtration and light scattering
      2. NMR determined Rotational correlation time
    4. Mass spectrum
    5. NMR screening


NMR Data Collection

  1. Routine operation
    1. NMR sample tubes
    2. NMR Sample Preparation
    3. Inserting NMR Sample
    4. Tuning and matching
    5. Deuterium Lock
    6. Shimming
    7. Pulse width calibration
    8. Temperature calibration
    9. Chemical shift referencing
  2. Advanced operation
    1. Deuterium pulse width calibration and decoupling
  3. NMR data acquisition for protein structure determination
    1. Common NMR experiment sets
    2. Custom NMR experiment setup scripts for VNMRJ
    3. 1D 1H NMR spectra and 2D [15N, 1H]-HSQC
    4. Estimation of rotational correlation time
    5. Estimation of measurement time
    6. NMR experiments for spin system identification
    7. 2D and 3D NOESY experiments
      1. Simultaneous 13C,15N-resolved NOESY
    8. Double and triple NMR experiments
      1. 3D CBCA(CO)NH and HNCACB
      2. 3D HNCA and HN(CO)CA
      3. 3D HAHB(CO)NH
      4. (4,3)D CABCA(CO)NH and HNCACB
      5. (4,3)D HABCAB(CO)NH
      6. (H)CCH
      7. (H)CCH-TOCSY
      8. H(C)CH
      9. H(C)CH-TOCSY
      10. (4,3)D HCCH
    9. Other NMR experiments
      1. 2D [13C, 1H]-HSQC for fractionally 13C-labeled samples
      2. Long-range 15N-1H correlation experiments for histidine rings - determination of histidine isoprotomer state
      3. MEXICO
      4. CLEANEX
      5. H-D exchange experiment
      6. 15N spin relaxation parameters
  4. Advanced problems for data collection
    1. Setting up non-uniformly sampled spectra
      1. Guide for Varian/BioPack
      2. Guide for Bruker/Topspin according to Arrowsmith group
  5. Maintenance
    1. VARIAN
      1. Installing and updating BioPack
      2. Full probefile calibration
      3. Rebooting spectrometer console
      4. Conditioning procedure for cryogenic probes
    2. BRUKER

NMR Data Processing

  1. Processing Procedures for Routine Experiments
    1. NMRPipe
      1. Brief description of philosophy, commands, and functions of NMRPipe
      2. Routine Processing Procedure for 2D Experiment
      3. Routine Processing Procedure for 3D 15N and 13C-edited Experiments
      4. 2D 15N HSQC-TROSY experiment for RDC measurement
      5. 2D J-modulation experiment for RDC measurement
      6. Routine processing of spectra using Buffalo-developed PERL scripts
    2. PROSA
    3. TOPSPIN
    4. AUTOPROC
    5. UBNMR
  2. Processing Procedures for Alternative Data Collection Methods
    1. G-Matrix Fourier transformation (GFT)
    2. Processing non-uniformly sampled spectra with Multidimensional Decomposition
  3. Spectra Format Conversion
    1. NMRPipe processed data conversion to Sparky, CARA, XEASY, and NMRViewJ
    2. TOPSPIN processed data conversion to Sparky, CARA, XEASY, and NMRViewJ

Resonance Assignment

This chapter focuses on data analysis and resonance assignment packages, as most people stick to a particular software for entire structure determination projects.


  1. Principles and concepts
  2. Practical aspects
    1. Semi-automated protocols
      1. CARA
        1. Spin System Identification in 2D 15N-HSQC and 3D HNNCO
        2. Backbone Resonance Assignment
        3. Assignment of HA and HB Resonances with (4,3)D GFT HABCAB(CO)NHN
        4. Side Chain Assignment
          1. Aliphatic side-chain assignment
          2. Aromatic side-chain assignment
          3. Amide side-chain assignment
      2. Sparky
      3. XEASY
        1. Introduction
        2. Spin system identification
        3. Backbone resonance assignment
          1. GFT-based spectra
            1. HNCACAB/CABCA(CO)NH
          2. Conventional spectra
            1. HNCACB/CBCA(CO)NH
        4. Side chain resonance assignment
          1. Aliphatic
            1. GFT-based spectra
              1. (4,3)D GFT HABCAB(CO)NHN
              2. (4,3)D GFT HCCH
            2. Conventional spectra
              1. HAHB(CO)NH
              2. HCCH-COSY
              3. Simultaneous NOESY
          2. Aromatic
          3. Other
            1. Trp e1 NH and d1 CH
            2. Met e CH3
            3. Asn d2 and Gln e2 NH2
        5. NOESY peak integration
    2. Automated protocols
      1. AutoAssign
      2. AutoAssign server
      3. ABACUS
      4. PINE server
    3. Validation of resonance assignment
      1. Assignment validation suite (AVS)
      2. Linear analysis of chemical shift (LACS)
    4. Depositing chemical shifts


Structure Calculation and Validation

  1. Principles and concepts
  2. Practical aspects
    1. Structure calculation
      1. CYANA
        1. Getting started
        2. FOUND
        3. TALOS
        4. GLOMSA
        5. NOE calibration
        6. Manual structure calculation
        7. Automated NOESY assignment and structure calculation
        8. Structure calculation with residual dipolar couplings (link to REDCAT/PALES,FINDTENSOR, .rdc file, adding ORI to PDB file)
        9. Homodimer structure calculations
      2. AutoStructure
        1. Getting started
        2. CYANA run
        3. XPLOR run
        4. Analyzing the output
        5. RPF/DP scores
        6. Structure calculation using AS-DP
      3. "Consensus" Approaches
        1. Overview of Consensus runs
        2. Finding Consensus NOE assignments
        3. Validation of Consensus runs
      4. CS-ROSETTA
      5. CS-DP ROSETTA
      6. CS-RDC-ROSETTA
      7. RDC-assisted dimer structure calculation
      8. Special topics
        1. Protein-Ligand complexes
        2. Metal ions
        3. Dimers
        4. Residual Dipolar Couplings
        5. REDCAT and REDCRAFT
        6. Paramagnetic constraints
    2. Structure Refinement
      1. CNS refinement
      2. XPLOR-NIH refinement
      3. ROSETTA refinement
    3. Validation and deposition
      1. PdbStat
      2. PSVS
      3. RPF analysis
      4. MolProbity server
      5. PDB and BMRB deposition
      6. ADIT-NMR
      7. HarvestDB
      8. SPINS

-- JeffMills - 28 May 2009

Here are two comments from Guy:

- need to have centralized site for downloading all software that NESG has developed or licensed; this would be a central site for NESG scientists to use to access the latest version of all software

- need to allow outside users to access links to all software (they will need licenses to download) and also to download software from NESG

-- AlexEletski - 13 Jul 2009