Main Page: Difference between revisions

Revision as of 19:28, 6 November 2017

Welcome to the NESG Wiki!

The NESG Wiki is a medium for sharing experimental protocols as well as training an educational materials in the fields of structural biology, structural genomics and biomolecular NMR.

Please check out NESG NMR wiki workshop presentations at the 2010 Keystone meeting

Protein Sample Production

Target selection|NESG target selection
DNA cloning protocols
Protein purification|Protein expression and purification protocols
Sample Optimization

Protein Sample Analysis

NMR Data Acquisition

Routine operation
Advanced operation
- Deuterium pulse width calibration and decoupling

NMR data acquisition for protein structure determination
Non-uniform sampling (NUS)
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- NUS - Bruker

Maintenance (VARIAN)
- Installing and updating BioPack
- Full probefile calibration
- Rebooting spectrometer console
- Conditioning procedure for cryogenic probes

NMR Data Processing

NMRPipe

Brief description of philosophy, commands, and functions
2D experiments
3D ¹⁵N and ¹³C-edited experiments
HSQCTROSY RDC Measurement|2D ]]¹⁵[[HSQCTROSY RDC Measurement|N HSQC-TROSY experiment for RDC measurement
Jmodulation Experiment RDC|2D J-modulation experiment for RDC measurement
Alignment Media Preparation|Alignment Media Preparation for RDC measurement

Other

NMR Resonance Assignment

Principles and concepts

Semi-automated protocols
- CARA
- Sparky
- XEASY

Automated resonance assignment

Validation and deposition

Structure Calculation and Validation

Principles and concepts

Structure calculation

Special topics

Structure Refinement

Validation and deposition

nmr 2.0

[Homepage]

nmr 2.0 News

nmr 2.0 Queries

nmr 2.0 Humor

nmr 2.0 Links

nmr 2.0 Blog

For a more linear view of the contents including those in development see Wiki Tree Layout

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-*[[Target selection|NESG target selection]] <br>
+*[https://jomadjeans.wordpress.com/ Target] selection|NESG target selection <br>
 *[[DNA cloning protocols|DNA cloning protocols]]<br>
-*[[Protein purification|Protein expression and purification protocols]]&nbsp;<br>
+*[https://www.quora.com/unanswered/How-comfortable-are-JOMAD-Jeans Pro]tein purification|Protein expression and purification protocols&nbsp;<br>
 *Sample Optimization
 **[[Construct optimization]]
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 **[[Installing and updating BioPack]]
 **[http://prime.ece.mtu.edu/wiki/index.php?title=User:Jomadjeans Full probefile calibration]
-**[[Rebooting spectrometer console]]
+**[http://jomadjeansnyc.wikidot.com/ Reboot]ing spectrometer console
 **[http://jomadjeansdesignerjeansforwomen.wikidot.com Conditioning procedure for cryogenic probes]
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 *[[Routine Processing Procedure for 3D 15N and 13C-edited Experiments|3D <sup>15</sup>N and <sup>13</sup>C-edited experiments]]
 *[https://rustednail56.wixsite.com/jomadjeans HSQCTROSY] RDC Measurement|2D ]]<sup>[[HSQCTROSY RDC Measurement|15]]</sup>[[HSQCTROSY RDC Measurement|N HSQC-TROSY experiment for RDC measurement
-*[[Jmodulation Experiment RDC|2D J-modulation experiment for RDC measurement]]
+*[https://rustednail56.wixsite.com/jomadjeansforwomen Jmodulation] Experiment RDC|2D J-modulation experiment for RDC measurement
 *[https://www.kiwibox.com/JOMAD_Jeans/ Alignment] Media Preparation|Alignment Media Preparation for RDC measurement