Main Page

Welcome to the NESG Wiki!

The NESG Wiki is a medium for sharing experimental protocols as well as training an educational materials in the fields of structural biology, structural genomics and biomolecular NMR.

Please check out NESG NMR wiki workshop presentations at the 2010 Keystone meeting

Protein Sample Production

Target selection|NESG target selection DNA cloning protocols Protein purification|Protein expression and purification protocols  Sample Optimization Construct optimization Buffer optimization Cofactor optimization

Protein Sample Analysis SDS page gel Protein concentration measurements Assessment of Oligomerization State gel-filtration and light scattering NMR determined Rotational correlation time Mass spectrum NMR screening

NMR Data Acquisition

Routine operation NMR Sample Preparation Inserting NMR Sample Tuning and matching Deuterium Lock Shimming Pulse width calibration Temperature calibration Chemical shift referencing Advanced operation Deuterium pulse width calibration and decoupling

NMR data acquisition for protein structure determination Common NMR experiment sets Custom NMR experiment setup scripts for VNMRJ Estimation of rotational correlation time High waisted flare jeans Simultaneous 13C,15N-resolved NOESY 2D [13C, 1H]-HSQC for fractionally 13C-labeled samples Ankle grazer jeans Non-uniform sampling (NUS) designer jeans for women NUS - Bruker

Maintenance (VARIAN) Installing and updating BioPack Full probefile calibration Rebooting spectrometer console Conditioning procedure for cryogenic probes

NMR Data Processing

NMRPipe Brief description of philosophy, commands, and functions 2D experiments 3D 15N and 13C-edited experiments HSQCTROSY RDC Measurement|2D ]]15[[HSQCTROSY RDC Measurement|N HSQC-TROSY experiment for RDC measurement Jmodulation Experiment RDC|2D J-modulation experiment for RDC measurement Alignment Media Preparation|Alignment Media Preparation for RDC measurement

Other PROSA TOPSPIN Fall Jeans Processing NUS spectra with MDD Spring Jeans

NMR Resonance Assignment

• Principles and concepts

Semi-automated protocols CARA Sparky XEASY

Automated resonance assignment AutoAssign AutoAssign server ABACUS PINE server

Validation and deposition Assignment validation suite (AVS) Linear analysis of chemical shift (LACS) Depositing chemical shifts

Structure Calculation and Validation

Principles and concepts

Structure calculation CYANA AutoStructure CS-ROSETTA CS-DP ROSETTA CS-RDC-ROSETTA "Consensus" approaches Refinement Strategies

Special topics Protein-Ligand complexes Metal ions Residual Dipolar Couplings REDCAT and REDCRAFT Paramagnetic constraints RDC-assisted dimer structure calculation

Structure Refinement CNS refinement XPLOR-NIH refinement ROSETTA refinement

Validation and deposition PdbStat PSVS RPF analysis MolProbity server RDCvis PDB and BMRB deposition ADIT-NMR HarvestDB SPINS

nmr 2.0

[Homepage]

nmr 2.0 Communication nmr 2.0 Educational nmr 2.0 Jobs nmr 2.0 Job Seekers nmr 2.0 News nmr 2.0 Queries nmr 2.0 Humor nmr 2.0 Links nmr 2.0 Blog

For a more linear view of the contents including those in development see Wiki Tree Layout

• 18 October protocol